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:: Volume 2, Issue 2 (2016) ::
pgr 2016, 2(2): 33-46 Back to browse issues page
Application of ISSR Molecular Markers for Genetic Diversity Study of Some Tobacco Genotypes
Marziyeh Shazdehahmadi * , Mahin Kharrazi
Instructor, Department of Biotechnology, Tirtash Tobacco Research and Education Center, Behshahr, Iran
Abstract:   (27791 Views)
Determination of genetic diversity of breeding material is the first step in breeding programs. Evaluation of tobacco genetic diversity is essential for breeding programs and preservation of genetic resources. Genetic diversity level in tobacco genotypes, is very important for selection of parents in breeding programs. In this study, genetic diversity of 100 tobacco genotypes was evaluated using 25 ISSR markers. Banding pattern based on the presence or absence of the bands showed with 0 and 1, respectively. Out of 237 fragments produced in total cultivars, 195 bands were polymorphic and average of polymorphism ranged from 4 to 12 per primer. Average of polymorphism percentage was 94.10. To determine the efficiency of ISSR markers, PIC and their polymorphic percentage was calculated. UBC 818, UBC 812 and UBC 815 had the best marker parameters and were introduced as the best primers for assessment of genetic diversity. In order to evaluate the genetic similarity between cultivars, different similarity coefficient (SM, Dice and Jaccard) was calculated and Mantel corresponding test was performed. Finally, dendrogram was drawn based on SM similarity coefficient and UPGMA algorithm and the Cofenetic coefficient was calculated. All genotypes formed two distinct clusters indicating the high efficiency of used primers in amplification the approximate parts of the genome. The principle coordinate analysis showed that the first three components could explain 79.65 % of total variance. Totally, evaluation the tobacco genetic diversity using ISSR markers is suitable and ISSR marker can be used as appropriate marker system to identify the diversity and genetic relationship for breeding programs of this plant.
Keywords: Cluster analysis, Genetic diversity, Tobacco, Polymorphism, ISSR marker.
Full-Text [PDF 598 kb]   (9557 Downloads)    
Type of Study: Research | Subject: Plant improvement
References
1. Arslan, B. and Okunus, A. (2006). Genetic and geographic polymorphism of cultivated tobacco (Nicotianatabacum) in Turkey. Russian Journal of Genetics, 42: 667-671.
2. Conolly, G.N., Alpert, H.R., Woyne, G.F. and Koh, H. (2007). Trends in nicotine yield in smoke and its relationships with design characteristics among popular US cigarette brands Tobaccoscience, 16: 11-25.
3. Darvishzadeh, R., Mirzaei, L., Hatami Maleki, H., Laurentin, H. and Alavi, S.R. (2013). Genetic variation in oriental tobacco (Nicotiana tabacum L.) by agromorphological traits and simple sequence repeat markers. Revista Ciência Agronômica, 44(2): 347-355.
4. Dellaporta, S.L., Wood, J. and Hicks, J.B. (1983). A plant DNA minipreparation: Version II. Plant Molecular Biology Reports, 1: 19-21.
5. Denduangboripant, J., Setaphan, S., Suwanprasart, W. and Panha, S. (2010). Determination of genetic diversity in local tobacco cultivars using ISSR molecular marker. Chiang Mai Journal of Science, 37(2): 293-303.
6. Edrisi Maryan, K., Samizadeh Lahiji, H. and Shoaei Deylami, M. (2012). Assessing the genetic diversity of tobacco (Nicotiana tabacum L.) Varieties. Crop Science, 14: 23-32.
7. FAO. (2010). FAOSTAT, Projections of tobacco production, consumption and trade to the year. Available at: http://faostat3.fao.org/home/E.
8. Gepts, P. (2006). Plant genetic resources conservation and utilization: The accomplishments and future of a societal insurance policy. Crop science, 46: 2278-2292.
9. Gholizadeh, S., Darvishzadeh, R., Abdollahi Mandoulakani, B., Bernousi, I., Alavi, R. and Khorabian, A. (2012). Molecular characterization and similarity relationships among Flue-cured tobacco (Nicotiana tabacumL.) genotypes using simple sequence repeat markers. Notulae Botanicae Horti Agrobotanici cluj-Napoca, 40(1): 247-253.
10. Hasani, F., Samizadeh Lahiji, H. and Shoae-Deylami, M. (2013). Assessment of genetic diversity between and within of tobacco genotypes using ISSR markers. Journal of Modern Genetics, 1: 1-12 (In Persian).
11. Hasani, F., Samizadeh Lahiji, H. and Shoae Deylami, M. (2015). Evaluation of genetic diversity between and within of tobacco genotypes using IRAP and REMAP markers. Journal of Modified Crop Plants, 16: 1-9.
12. Li, Q., Liu, Q.C., Zhai, H., Ma, D.F., Wang, X., Li, X.Q. and Wang, Y.P. (2008). Genetic diversity in main parents of Sweet Potato in China as revealed by ISSR markers. Acta Agronomica Sinica, 34: 972-977.
13. Mantel, N. (1967). The detection of disease clustering and a generalized regression approach. Cancer Research, 27: 209-220.
14. Mohsenzadeh Golfazani, M., Samizadeh Lahiji, H., Alami, A., Shoae Deylami, M. and Talesh Sasani, S. (2012). Study of genetic diversity of different flue-cured tobacco genotypes using ISSR marker and Retrotransposons. Iranian Journal of Field Crop Science, 43: 371-380 (In Persian).
15. Mwadzingeni, L., Kashangura, C., Gasura, E., Garwe, D. and Lewis, R. (2013). Genetic diversity of Zimbawean and exotic flue-cured tobacco varieties based on phenotypic traits and simple sequence repeats. African Journal of Agricultural Research, 8(46): 5845-5853.
16. Powell, W., Margenta, M., Andre, C., Hanfrey, M., Vogel, J., Tingey, S. and Rafalsky, A. (1996). The utility of RFLP, RAPD, AFLP and SSR markers for germplasm analysis. Molecular Breeding, 2: 225-238.
17. Qi, J.M., Liang, J.X, Chen, M.X., Xu, J.T., Niu, X.P., Zhou, D.X., Wang, T. and Chen, S.H. (2006). Genetic diversity and genetic relative’s analysis of tobacco germplasm based on InterSimple Sequence Repeat (ISSR). Acta Agronomica Sinica, 32: 373-378.
18. Reddy, P.M., Sarla, N. and Siddig, E.A. (2002). Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, 128: 9-17.
19. Ren, N. and Timko, M.P. (2001). AFLP analysis of genetic polymorphism and evolutionary relationships among cultivated and wild Nicotiana species. Genetics, 44: 559-571.
20. Samizadeh Lahiji, H., Mohsenzadeh Golfazani, M., Edrisi Maryan, K., Shoaei Deylami, M. and Alami, A. (2013). Assessing the genetic diversity of 89 flue-cured tobacco varieties using morphological traits and inter-simple sequence repeat markers. Journal of Crop Breeding, 3(2): 79-85 (In Persian).
21. Sarala, K. and Rao, R.V.S. (2008). Genetic diversity in Indian FCV and burley tobacco cultivars. Journal of Genetics, 2: 159-163.
22. Tao, A.F., Liu, Z.H., Qi, J.M., Zhou, D.X., Wang, T. and Chen, SH.H. (2009). Comparison and analysis of IRAP and ISSR molecular methods used for assessment of genetic diversity in tobacco. Journal of Wuhan Botanical Research, 27(6): 589-594.
23. Yang, B.C., Xiao, B.G., Chen, X.J. and Shi, C.H. (2005). Genetic diversity of flue-cured tobacco varieties based on ISSR markers. Yi Chuan-Hereditas, 27:753-758.
24. Yang, B.C., Xiao, B.G., Chen, X.J. and Shi, C.H. (2007). Assessing the genetic diversity of tobacco germplasm using inter-simple sequence repeat and inter-retrotransposon amplification polymorphism markers. Annals of Applied Biology, 150: 393-401.
25. Zahng, H.Y., Liu, X.Z., He, C.S. and Yang, Y.M. (2008). Genetic diversity among Flue-cured tobacco based on RAPD and AFLP markers. Brazilian Archives of Biology and Technology, 51: 1097-1101.



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Shazdehahmadi M, Kharrazi M. Application of ISSR Molecular Markers for Genetic Diversity Study of Some Tobacco Genotypes. pgr 2016; 2 (2) :33-46
URL: http://pgr.lu.ac.ir/article-1-46-en.html


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Volume 2, Issue 2 (2016) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
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