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Investigation of Genetic Diversity of Some Iranian Violet Species (Viola sp.) based on iPBS Molecular Marker
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Faraneh Roshan  , Mohammad Rabiei *  , Behrouz Shiran |
| Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran , rabiei@sku.ac.ir |
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Abstract: (109 Views) |
Violet plants (Viola sp.) belonging to the Violaceae family are ornamental plants that could be used for drug design due to their cyclotidic compounds. In this study, 21 different ecotypes of violets were collected from the northern regions of Iran. After DNA extraction, the genetic diversity of ecotypes was investigated using the iPBS molecular marker. Twelve iPBS primers used during the present investigation resulted in 214 bands. The average percentage of observed polymorphism, polymorphic information content (PIC), and marker index (MI) were calculated to be 31.92%, 0.35%, and 5.64% respectively. The Nei genetic distance index ranged between 0 and 0.66. The results of this study indicated a considerable genetic diversity among the violet ecotypes and the efficiency of the iPBS marker in detecting polymorphism. The population genetic analysis showed that 61% of the diversity was associated with intra-species diversity. The species V. odorata and V. alba exhibited the greatest degrees of polymorphism, effective allele number, Shannon index value, and heterozygosity ratios. Also, the dendrogram depicted the close genetic relationship between these two species, as evidenced by Nei's genetic distance measurements. In general, considering the existing taxonomic information and the results obtained from this experiment, it can be concluded that the use of the iPBS marker was highly effective in systematic studies of the genus Viola. The results of this experiment led to the appropriate differentiation of ecotypes and species, which could be used in further breeding studies. |
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| Keywords: Polymorphism, Genetic distance, Interspecies analysis |
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Type of Study: Research |
Subject:
Molecular genetics
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