Studying of Genetic Diversity of rbcL Gene in Some Date Palm Cultivars in Sistan and Baluchistan Province, Iran

Raeisi, Fatemeh; Fahmideh, Leila; Fakheri, Barat Ali; Kikhasaber, Mojtaba

doi:10.52547/pgr.8.1.3

[Home ] [Archive]

[ فارسی ]

Main Menu

Home

Journal Information

Articles archive

For Authors

For Reviewers

Registration

Contact us

Site Facilities

Search in website

Receive site information

Volume 8, Issue 1 (2021)

pgr 2021, 8(1): 29-42

Back to browse issues page

Studying of Genetic Diversity of rbcL Gene in Some Date Palm Cultivars in Sistan and Baluchistan Province, Iran

Fatemeh Raeisi

, Leila Fahmideh ^*

, Barat Ali Fakheri

, Mojtaba Kikhasaber

Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran , l.fahmideh@gau.ac.ir

Abstract: (5673 Views)

Due to the importance of date palm production in Iran, introduction of new cultivars, improvement of current cultivars and studying the genetic diversity among available cultivars is essential for improvement the quantity and quality of date palm production. In this research, 15 different local genotypes of date palm collected from Saravan, Jalgh, Nahook and Sinokan regions of Sistan and Baluchistan province were used for diversity analysis. To this end, DNA was extracted from leaves using Delaporta method and DNA quality and quantity were determined using spectrophotometer and 1% gel electrophoresis. PCR was performed using specific rbcL primers under determined conditions and the amplicons were sequenced. To study the relationships and genetic distances between genotypes, the results of sequencing were analyzed and dendrogram of phylogenetic relationships as well as sequence similarity matrix were generated using Bioedit, and MEGA7 software. The results of the present study showed that there were a total of 553 different residues for this marker of which 505 residues contained deletion and addition, and 48 residues were without deletion and addition. The genetic distance ranged from 0.0 to 0.037 and the highest intra-regional diversity was related to cultivar Jm13_sabzoo. Based on the dendrogram obtained from cluster analysis, the studied cultivars were divided into two branches, in which the first branch contained cultivar Sabzoo from Jalgh region and the other cultivars were grouped in different sub-branches of second branch. Although the rbcL marker is useful for studying and recognizing diversity of intraspecific relationships, a low genetic distance was estimated for the studied date palm genotypes. However, it is suggested that the other DNA barcodes as well the other appropriate molecular markers could be used for future studies of date palm genetic diversity.

Keywords: Genetic diversity, Date palm, Phylogenetic relationships, rbcL markers

Full-Text [PDF 904 kb] (1103 Downloads)

Type of Study: Research | Subject: Molecular genetics
Accepted: 2021/03/2

References

1. Akkak, A., Scariot, V., TorelloMarinoni, D., Boccacci, P., Beltramo, C. and Botta, R. (2009). Development and evaluation of microsatellite markers in Phoenix dactylifera and their transferability to other Phoenix species. Biologia Plant Arum, 53: 164-166.

2. Dellaporta, S.L., Wood, J. and Hicks, J.B. (1993). A plant DNA minipreparation: version II. Plant Molecular Biology Reporter, 1: 19-22.

3. Elmeer, K.H., Sarwath, J., Malek, J., Baum, M. and Hamwieh, A. (2011). New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.). Biotechnology, 1: 91-97.

4. Geilly, L. and Taberlet P. (1994). The use of chloroplast DNA to resolve plant phylogenies: noncoding versus rbcL sequences. Molecular Biology and Evolution, 11: 769-777.

5. Ghahramanzadeh, R., Marashi S.H., Wael, C. and Malekzadeh, S. (2012). Separation of invasive species of aquatic herb Mriiophyllum (Myriophyllum spp.) From its native relatives using DNA barcode. Plant Protection Quarterly, 26: 101-109.

6. Gupta, P., Varshney, R., Sharma, P. and Ramesh, B. (1999). Molecular markers and their application in wheat breeding. Plant Breeding, 118: 360-390.

7. Hashempour, M. )1999.( Date Treasure. Agricultural Education affiliated with the Office of Educational Technology Services of the Ministry of Jihad Agriculture, Tehran, IR (In Persian).

8. Hashemi Sistani, M. )2018(. Molecular analysis of some Mango genotypes by using the sequencing of the rbcL gene. M.Sc. Thesis, Faculty of Agriculture, Higher Education Complex of Shirvan, Shirvan, Iran (In Persian).

9. Hosseini, S.Z., Shiran, B. and Ebrahimi M.A. (2018). Study on phylogenetic relationship among some of Iranian wild almond species using sequences of ITS1-5.8S rDNA-ITS2 Region and chloroplastic trnL. Plant Genetic Researches, 5(2):17-28 (In Persian).

10. Khan, S., Khan, Z., Ashfaq, M. and Nisa, Z. )2016(. Identification of the grass family (Poaceae) by using the plant dna barcodes rbcL and matK. Journal of Biodiversity and Environmental Sciences, 8: 175-186.

11. Lewin, G.R., Rueff, A. and Mendell, L.M. )1994(. Peripheral and central mechanisms of NGF‐induced hyperalgesia. European Journal of Neuroscience, 6: 1903-1912.‏

12. Li, J., Bi, H., Li, Z. and Feng, J. )2008(. Genetic analysis of Ziziphus Jujube Huizao using ISSR markers. 1th International Jujube Symposium, Baoding, China.

13. Marsefri, M. and Mehrabi, A. )2010(. Evaluation of Genetic diversity of some Iranian date populations using fingerprint identification (ISSR). National Conference and Iranian Scientific Date Festival, Kerman, Shahid Bahonar University of Kerman, Kerman, Iran (In Persian).

14. MirMohammadi Maibody, S.A.M. and Golkar, P. (2019). Application of DNA molecular markers in plant breeding. Plant Genetic Researches, 6(1):1-30 (In Persian).

15. Nejad Habib, R. )2013(. Evaluation of genetic diversity within and between male and female date palms using microsatellite markers. M.Sc. Thesis, Faculty of Agriculture, University of Zabol, Zabol, Iran (In Persian).

16. Pang, X., Luo, H. and Sun, C. )2012(. Assessing the potential of candidate DNA barcodes for identifying non-flowering seed plants. Plant Biology, 14: 839-844.

17. Pejman, H. (2001). Date Guide (Planting, Holding, Harvesting). Agricultural Education and Extension, Tehran, IR (In Persian).

18. Taghinejad, H., Fahmideh, L., Samsampoor, D. and Askaryesyahooi, M. (2018). Genetic diversity of date palm cultivars in Sistan and Baluchistan and Hormozgan provinces using microsatellite. Journal of Molecular and Cellular Research, 31: 221-231 (In Persian).

19. Tamura, K. and Nei, M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology Evolution, 10: 512-526.

20. Thakur, V., Tiwari, S.H.T., Tripathi, N., Tiwari, G. and Sapre, S. (2016). DNA barcoding and phylogenetic analyses of Mentha species using rbcL sequences. Annals of Phytomedicine, 5: 59-62.

21. Varma, P., Talwar, S. and Gary, G. (1980). Chemical investigation of Silybum marianum. Palm Medica, 38: 377-378.

Send email to the article author

Add your comments about this article

‎ 10.52547/pgr.8.1.3

‎ 20.1001.1.23831367.1400.8.1.4.8

Mendeley

Zotero

RefWorks

Raeisi F, Fahmideh L, Fakheri B A, Kikhasaber M. Studying of Genetic Diversity of rbcL Gene in Some Date Palm Cultivars in Sistan and Baluchistan Province, Iran. pgr 2021; 8 (1) :29-42
URL: http://pgr.lu.ac.ir/article-1-185-en.html

Rights and permissions
	This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Volume 8, Issue 1 (2021)

Back to browse issues page

Persian site map - English site map - Created in 0.08 seconds with 39 queries by YEKTAWEB 4642