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:: Volume 8, Issue 1 (2021) ::
pgr 2021, 8(1): 17-28 Back to browse issues page
Assessment of Genetic Diversity between Different Accessions of Calotropis Procera with ISSR Molecular Markers
Saeid Navabpour * , Ahad Yamchi , Sasan Golcheshmeh
Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran , s.navabpour@gau.ac.ir
Abstract:   (7610 Views)
The present study was performed to classify and study genetic diversity between Calotropis procera accessions from different regions of Kerman province (Iran) using ISSR markers. In total, DNA from 14 plant samples with nine ISSR primers was amplified by PCR and their banding pattern was obtained. The primers showed acceptable polymorphism (35.93) and minimum and maximum polymorphic information content (PIC) of primers in this study were 0.11 for ISSR9 primer and 0.41 for ISSR3 and ISSR8 primers, respectively. Genetic similarity based on Nei index was varied from 0.405 to 0.745 and the lowest genetic similarity was found between J3 (Related to Jiroft) and D2 (Related to Dosari) and the highest genetic similarity was found between J1 and J2 (both of them for Jiroft). By using UPGMA cluster analysis, samples divided into four groups, and the second and third groups contained more accessions. In terms of genetic similarity, two accessions of Jiroft 1 (J1) and Jiroft 2 (J2) which classified in the same cluster were closer. Also, the accessions collected from Anbarabad were at a longer genetic distance than other accessions. Principal coordinate analysis also showed that the first and second components justify 67 percent of obtained genetic diversity. In general, ISSR markers were useful for classifying Calotropis procera accessions and according to the obtained information about existence of genetic diversity between Calotropis procera accessions of Kerman province, this diversity could be useful in the future for breeding and production of Calotropis procera.
Keywords: Polymorphism, Genetic resources, Genetic distance, Medicinal plant, DNA marker
Full-Text [PDF 507 kb]   (2176 Downloads)    
Type of Study: Research | Subject: Molecular genetics
References
1. Abbasi, H.A., Tasaodi, S., Mirsoleimani, M.A. and Masoudi, M. (2016). Study of milkweed (Calotropis procera) regeneration potential in vitro culture conditions. Journal of Plant Researches, 29: 833-842 (In Persian).
2. Agossou-Yao, D.A.R., Sprycha, Y., Porembski, S. and Horn, R. (2015). AFLP assessment of the genetic diversity of Calotropis procera (Apocynaceae) in the West Africa region (Benin). Genetic Resources and Crop Evolution, 62: 863-878.
3. Ahmadi-Khah, A. (2011). Advanced Genetic, 2nd. Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran (In Persian).
4. Ahmed, A., El-Bakry, A., Hammad, I.A. and Rafat, F.A. (2014). Polymorphism in Calotropis procera: preliminary genetic variation in plants from different phytogeographical regions of Egypt. Rendiconti Lincei Scienze Fisiche Naturali, 25: 471-477.
5. Al-Yemni, M.N., Sher, H., El-Sheikh, M.A. and Eid, E.M. (2011). Bioaccumulation of nutrient and heavy metals by Calotropis procera and Citrullus colocynthis and their potential use as contamination indicators. Scientific Research and Essays, 6: 966-976.
6. Amiri, P., Ismaili, A. and Hadian, J. (2017). Evaluation of genetic diversity of Styrian pumpkin (Cucurbita pepo var. styriaca) populations, using ISSR molecular markers. Plant Genetic Researches, 4(2): 17-28 (In Persian).
7. Anderson, J.A., Churchill, G.A., Autrique, J.E., Tanksley, S.D. and Sorrells, M.E. (1993). Optimizing parental selection for genetic linkage maps. Genome, 36: 181-186.
8. Ash, G.H., Raman, R. and Crump, N.S. (2002). An investigation of genetic variation in Carthamus lanatus in New South Wales, Australia, using inter simple sequence repeats (ISSR) analysis. European Weed Research Society, 43: 208-213.
9. Babakhani, M. and Yavari, A. (2018). Calotropis procera Willd. medicinal plant, ideal for afforestation in the Persian Gulf and Oman sea. 1st National Conference on Novel Ideas in Agriculture and Natural Resources, University of Mohaghegh Ardabili, Iran (In Persian).
10. Doyle, J. and Doyle, J. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11-15.
11. Farshadfar, E. (1998). Application of Biometrical Genetics in Plant Breeding (I and II), Razi University of Kermanshah, Kermanshah, Iran (In Persian).
12. Ghandehari, V., Ahmadi-Khah, A. and Payamnoor, V. (2013). Genetic diversity of Buxus hyrcana populations in north of Iran using ISSR markers. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 21: 1-13 (In Persian).
13. Golcheshmeh, S., Pahlavani, M.H., Esmaeilzadeh-Moghaddam, M. and Zaynali-Nezhad, K. (2019). Morphological evaluation and marker assisted for tillering of wheat. Journal of Crop Breeding, 11: 153-160 (In Persian).
14. Hoisington, D., Khairallah, M., Reeves, T., Ribaut, J.M., Skovmand, M., Taba, S. and Warburton, M. (1999). Plant genetic resources: What can they contribute toward increased crop productivity? National Academy of Sciences, 96: 5937-5943.
15. Kakaei, M., Mazahery-Laghab, H. and Kahrizi, D. (2013). Study of morphological and biochemical to determine the genetic diversity of cultivated Oat (Avena sativa L.). Agricultural Biotechnology Journal, 5: 119-138 (In Persian).
16. Khorshidi, J., Shokrpour, M. and Nazeri, V. (2018). Assessment of genetic diversity in different populations of Thymus daenensis Celak. using ISSR marker. Journal of Agricultural Biotechnology, 10: 59-74 (In Persian).
17. Krasteva, L. (2000). Organization of Melon plant genetic resources in Bulgaria. Acta Horticulturae, 510: 247-25.
18. Mondak, B. (2013). Study of hybridization, genetic and phytochemical diversity in native Thyme of Iran. M.Sc. Thesis, University of Tehran, Tehran, Iran.
19. Nei, M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Center for Demographic and Population Genetics, 89: 583-590.
20. Padhy, B.M., Srivastava, A. and Kumar, V.L. (2007). Calotropis procera latex affords protection against carbon tetrachloride induced hepatotoxicity in rats. Journal of Ethnopharmacology, 113: 498-502.
21. Raghavan, R.S. (1957). Chromosome number in Indian medicinal plants. In Proceedings of the Indian Academy of Sciences-Section B, 45: 294-298.
22. Rahmati, H. and Shirvani, H. (2018). The study of genetic diversity Dactylis glumerata ecotype using ISSR molecular marker. Iranian Journal of Biology, 31: 1-9 (In Persian).
23. Ramezani, M. and Rahimi, M. (2017). Grouping and estimation of genetic diversity of different ecotypes of medicinal plant of Plantago psyllium using ISSR marker. Journal of Cellular and Molecular Researches, 30: 141-151 (In Persian).
24. Reddy, M.P., Sarla, N. and Siddiq, E.A. (2002). Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, 128: 9-17.
25. Ribaut, J.M., Banziger, M. and Hoisington, D. (2002). Use of Molecular Markers in Plant Breeding: Drought Tolerance Improvement in Tropical Maize. In: Kang, M.S., Ed., Quantitative Genetics, Genomics and Plant Breeding, pp. 85-100. Louisiana State University, Baton Rouge, USA.
26. Sabzalian, M.R., Mirlohi, A., Saeidi, G. and Rabbani, M.T. (2009). Genetic variation among population of wild safflower, Carthamus oxyacanthus analyzed by agro-morphological traits and ISSR markers. Genetic Resources and Crop Evolution, 56: 1057-1064.
27. Salami, A., Pahlevani, M., Zaynali-Nezhad, K. and Esmaeilzadeh Moghaddam, M. (2018). Genetic variation pattern of Iranian wheat landraces based on ISSR molecular markers and morphological traits. Plant Genetic Researches, 5: 87-100 (In Persian).
28. Soltani-Eini, M., Razmi, G.H., Afkhami, A. and Ahmadi, A. (2012). Therapeutic effects of liquid, hydro alcohol extracts of Calotropis spp. and Butalex, against Haemoproteus spp. infection in Pigeons. Journal of Veterinary Microbiology, 8: 101-107 (In Persian).
29. Yang, B.C., Xiao, B.G., Chen, X.J. and Shi, C.H. (2005). Genetic diversity of flue-cured tobacco varieties based on ISSR markers. Yi Chuan Hereditas, 27: 753-758.
30. Yang, Y.X., Wu, W., Zheng, Y.L., Chen, L., Liu, R.J. and Huang, C.Y. (2007). Genetic diversity and relationships among safflower (Carthamus tinctorius L.) analyzed by inter-simple sequence repeats (ISSRs). Genetic Resources and Crop Evolution, 54: 1043-1051.
31. Ying-Ben, L., Yang, L., Yi-Chun, T.I., Li-Yuang, W., Hao, C. and Ping-Seng, W. (2010). Assessment of genetic diversity and relationship of Tea germplasm in Yunnan as revealed by ISSR markers. Acta Agronomica Sinica, 36: 391-400.
32. Zamani, Z., Sarkhosh, A., Fattahi-Moghaddam, M.R. and Ebadi, A. (2005). Comparison of six genomic DNA extraction methods from pomegranate (Punica granatum L.). Iranian Journal of Horticultural Science and Technology, 6: 99-110 (In Persian).
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Navabpour S, Yamchi A, Golcheshmeh S. Assessment of Genetic Diversity between Different Accessions of Calotropis Procera with ISSR Molecular Markers. pgr 2021; 8 (1) :17-28
URL: http://pgr.lu.ac.ir/article-1-183-en.html


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Volume 8, Issue 1 (2021) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
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