Assessment of Genetic Diversity Among some Oilseed Rape (Brassica nupus L.)
Plants, using Single Sequence Repeats (SSR) Molecular Markers

Karimbeigi, Hadi; Nazarian-Firouzabadi, Farhad; Khademi, Mitra; Mousav, Elham

doi:10.29252/pgr.3.1.45

[Home ] [Archive]

[ فارسی ]

Main Menu

Home

Journal Information

Articles archive

For Authors

For Reviewers

Registration

Contact us

Site Facilities

Search in website

Receive site information

Volume 3, Issue 1 (2016)

pgr 2016, 3(1): 45-56

Back to browse issues page

Assessment of Genetic Diversity Among some Oilseed Rape (Brassica nupus L.) Plants, using Single Sequence Repeats (SSR) Molecular Markers

Hadi Karimbeigi

, Farhad Nazarian-Firouzabadi ^*

, Mitra Khademi

, Elham Mousav

Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

Abstract: (17892 Views)

Oilseed rape (Brassica nupus L), a member of Brassicaceae family, is an important crop regarding oil production worldwide. Brassicaceae is an economically important family of flowering plants with about 350 genera and more than 3000 species. Eleven pairs of single sequence repeat (SSR) primers were used to identify the genetic diversity among 21 oilseed rape genotypes. Results of SSR molecular marker analysis revealed that SSR primers produced a total number of 76 scorable bands of which 46 (60.5%) bands were polymorphics. The average number of bands for each primer and genotype was 6.9 and 3.6, respectively. Both CB10036B and Na10A09 primers produced 10 and Cb10403 primer produced 4 polymorphic bands, respectively. UPGMA cluster analysis based on Dice similarity matrix showed that Zarfam and Gerinimo genotypes had the highest (0.99%) and Licord and KS-11 genotypes had the lowest (0.72%) similarity. Both Iranian and foreign genotypes were grouped together in one major cluster, indicating presumably they may have the same origin and/or common pedigree. Results of AMOVA analysis within and between groups (spring – Autumn) revealed that almost 97% of total genetic diversity belonged to within group genotypes.

Keywords: Microsatellite, Canola, Clustering, Polymerase chain reaction

Full-Text [PDF 589 kb] (2577 Downloads)

Type of Study: Research | Subject: Plant improvement

References

1. Ash, M. and Dohlman, E. (2006). Oil crops situation and outlook yearbook. Electronic outlook report from the economic research service. US Department of Agriculture. USA.

2. Azizi, M. and Khavariy-Khorasani, S. (1999). Canola physiology, agronomy, plant breeding and biotechnology. One Printing, Jahad Daneshgahi of Mashhad. Mashhad, Iran (In Persian).

3. Butruille, D.V., Guries, R.P. and Osborn, T.C. (1999). Linkage analysis of molecular markers and quantitative trait loci in populations of inbred backcross lines of Brassica napus L. Genetics, 153: 949-964.

4. Condit, R. and Hubbell, S.P. (1991). Abundance and DNA sequence of two-base repeat regions in tropical tree genomes. Genome, 34: 66-71.

5. Darvishnia, F., Fakheri, B.A., Nazarian-Firouzabadi, F. and Panjehkeh, N. (2015). Genetic diversity of rapeseed (Brassica napus L.) using SSR and ISJ molecular markers. Journal of Field Crop Science, 46(1): 1-14 (In Persian).

6. Darvishian, A., Ismaili, A., Nazarian-Firouzabadi, F. and Mirdrikvand, R. (2016). Assessment of genetic diversity among wheat genotypes of west Iran, using randomized markers. Plant Genetic Researches, 2(2): 47-56 (In Persian).

7. Dellaporta, S.L., Wood, J. and Hicks, J.B. (1983) A plant DNA minipreparation: version II. Plant Molecular Biology Reporter, 1: 19-21.

8. Duran, C., Appleby, N., Edwards, D. and Batley, J. (2009) Molecular genetic markers: discoveryapplications, data storage and visualisation. Current Bioinformatics, 4: 6-27.

9. Farzin, A., Nourmohammadi, G. and Shiranirad, A.H. (2006). An investigation on quantitative and qualitative characters of 25 winter Rapeseed cultivars. Journal of Agricultural Sciences, 12(2): 429-437.

10. Goldstein, D.B. and Schlotterer, C. (1999). Microsatellites: evolution and applications. Oxford university press. UK.

11. Quijada, P.A., Udall, J.A., Polewicz, H., Vogelzang, R.D. and Osborn, T.C. (2004). Phenotypic effects of introgressing French winter germplasm into hybrid spring canola. Crop Science, 44(6): 1982-1989.

12. Hasan, M., Seyis, F., Badani, A., Pons-Kühnemann, J., Friedt, W., Lühs, W. and Snowdon, R. (2006). Analysis of genetic diversity in the Brassica napus L. gene pool using SSR markers. Genetic Resources and Crop Evolution, 53: 793-802.

13. Lowe, A.J., Jones, A.E., Raybould, A.F., Trick, M., Moule, C.L. and Edwards, K.J. (2002). Transferability and genome specificity of a new set of microsatellite primers among Brassica species of the U triangle. Molecular Ecology Notes, 2: 7-11.

14. Maguire, T., Peakall, R. and Saenger, P. (2002). Comparative analysis of genetic diversity in the mangrove species Avicennia marina (Forsk.) Vierh. (Avicenniaceae) detected by AFLPs and SSRs. Theoretical and Applied Genetics, 104: 388-398.

15. Maliro, D. (2011). Comparison of Agricultural Input Subsidies and Social Cash Transfersas Policies for Reducing Vulnerability to Hunger in Malawi. University of East Anglia, UK.

16. Mohammadi, S.A., Mohebalipour, N., Toorchi, M., Aharizad, S. and Javidfar, F. (2009). Assessment of genetic diversity in rapeseed cultivars as revealed by RAPD and microsatellite markers. African Journal of Biotechnology, 8: 3160-3167.

17. Mohammadi, S. and Prasanna, B. (2003). Analysis of genetic diversity in crop plants-salient statistical tools and considerations. Crop Science, 43: 1235-1248.

18. O'Donoughue, L., Souza, E., Tanksley, S. and Sorrells, M. (1994). Relationships among North American oat cultivars based on restriction fragment length polymorphisms. Crop Science, 34: 1251-1258.

19. Panaud, O., Chen, X. and McCouch, S.R. (1995). Frequency of microsatellite sequences in rice (Oryza sativa L.). Genome, 38: 1170-1176.

20. Piquemal, J., Cinquin, E., Couton, F., Rondeau, C., Seignoret, E., Perret, D., Villeger, M.J., Vincourt, P. and Blanchard, P. (2005). Construction of an oilseed rape (Brassica napus L.) genetic map with SSR markers. Theoretical and Applied Genetics, 111:1514-1523.

21. Plieske, J. and Struss, D. (2001). Microsatellite markers for genome analysis in Brassica. I. Development in Brassica napus and abundance in Brassicaceae species. Theoretical and Applied Genetics, 102: 689-694.

22. Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S. and Rafalski, A. (1996). The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2: 225-238.

23. Quijada, P.A., Udall, J.A., Lambert, B. and Osborn, T.C. (2006). Quantitative trait analysis of seed yield and other complex traits in hybrid spring rapeseed (Brassica napus L.): 1. Identification of genomic regions from winter germplasm. Theoretical and Applied Genetics, 113: 549-561.

24. Rohlf, F.J. (1992). NTSYS-pc: numerical taxonomy and multivariate analysis system. Applied Biostatistics. Setauket, New York, USA.

25. Rudolph, B., Uzunova, M. and Ecke, W. (2000). Development of microsatellite markers for the analysis of genetic diversity in rapeseed (Brassica napus L.), 3rd ISHS International Symposium on Brassica, 12th Crucifer Genetics Workshop, AUS.

26. Schlötterer, C. and Tautz, D. (1992). Slippage synthesis of simple sequence DNA. Nucleic Acids Research, 20: 211-215.

27. Smith, G.P. (1976). Evolution of repeated DNA sequences by unequal crossover. Science, 191: 528-535.

28. Tonguç, M. and Griffiths, P.D. (2004). Genetic relationships of Brassica vegetables determined using database derived simple sequence repeats. Euphytica, 137: 193-201.

29. Udall, J.A., Quijada, P.A., Lambert, B. and Osborn. T.C. (2006). Quantitative trait analysis of seed yield and other complex traits in hybrid spring rapeseed (Brassica napus L.): 2. Identification of alleles from unadapted germplasm. Theoretical and Applied Genetics, 113: 597-609.

30. Uzunova, M. and Ecke, W. (1999). Abundance, polymorphism and genetic mapping of microsatellites in oilseed rape (Brassica napus L.). Plant Breeding, 118: 323-326.

31. Wang, J., Kaur, S., Cogan, N., Dobrowolski, M.P., Salisbury, P., Burton, W., Baillie, R., Hand M., Hopkins, C. and Forster, J. (2009). Assessment of genetic diversity in Australian canola (Brassica napus L.) cultivars using SSR markers. Crop and Pasture Science, 60: 1193-1201.

32. Yim, G., Ding, D., Wang, C., Leong, W. and Hong, Y. (2009). Microsatellite markers to complement distinctness, uniformity, stability testing of Brassica chinensis (Xiao Baicai) varieties. The Open Horticulture Journal, 2: 54-61.

33. Yu, C., Hu, S., Zhao, H., Guo, A. and Sun, G. (2005). Genetic distances revealed by morphological characters, isozymes, proteins and RAPD markers and their relationships with hybrid performance in oilseed rape (Brassica napus L.). Theoretical and Applied Genetics, 110: 511-518.

34. Zhou, W., Zhang, G., Tuvesson, S., Dayteg, C. and Gertsson, B. (2006). Genetic survey of Chinese and Swedish oilseed rape (Brassica napus L.) by simple sequence repeats (SSRs). Genetic Resources and Crop Evolution, 53: 443-447.

‎ 10.29252/pgr.3.1.45

‎ 20.1001.1.23831367.1395.3.1.4.3

Mendeley

Zotero

RefWorks

Karimbeigi H, Nazarian-Firouzabadi F, Khademi M, Mousav E. Assessment of Genetic Diversity Among some Oilseed Rape (Brassica nupus L.) Plants, using Single Sequence Repeats (SSR) Molecular Markers. pgr 2016; 3 (1) :45-56
URL: http://pgr.lu.ac.ir/article-1-88-en.html

Rights and permissions
	This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Volume 3, Issue 1 (2016)

Back to browse issues page

Persian site map - English site map - Created in 0.06 seconds with 38 queries by YEKTAWEB 4657