Assessment of Genetic Diversity Among some Oilseed Rape (Brassica nupus L.)
Plants, using Single Sequence Repeats (SSR) Molecular Markers

Karimbeigi, Hadi; Nazarian-Firouzabadi, Farhad; Khademi, Mitra; Mousav, Elham

doi:10.29252/pgr.3.1.45

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Volume 3, Issue 1 (2016)

pgr 2016, 3(1): 45-56

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Assessment of Genetic Diversity Among some Oilseed Rape (Brassica nupus L.) Plants, using Single Sequence Repeats (SSR) Molecular Markers

Hadi Karimbeigi

, Farhad Nazarian-Firouzabadi ^*

, Mitra Khademi

, Elham Mousav

Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

Abstract: (18128 Views)

Oilseed rape (Brassica nupus L), a member of Brassicaceae family, is an important crop regarding oil production worldwide. Brassicaceae is an economically important family of flowering plants with about 350 genera and more than 3000 species. Eleven pairs of single sequence repeat (SSR) primers were used to identify the genetic diversity among 21 oilseed rape genotypes. Results of SSR molecular marker analysis revealed that SSR primers produced a total number of 76 scorable bands of which 46 (60.5%) bands were polymorphics. The average number of bands for each primer and genotype was 6.9 and 3.6, respectively. Both CB10036B and Na10A09 primers produced 10 and Cb10403 primer produced 4 polymorphic bands, respectively. UPGMA cluster analysis based on Dice similarity matrix showed that Zarfam and Gerinimo genotypes had the highest (0.99%) and Licord and KS-11 genotypes had the lowest (0.72%) similarity. Both Iranian and foreign genotypes were grouped together in one major cluster, indicating presumably they may have the same origin and/or common pedigree. Results of AMOVA analysis within and between groups (spring – Autumn) revealed that almost 97% of total genetic diversity belonged to within group genotypes.

Keywords: Microsatellite, Canola, Clustering, Polymerase chain reaction

Full-Text [PDF 589 kb] (2637 Downloads)

Type of Study: Research | Subject: Plant improvement

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‎ 10.29252/pgr.3.1.45

‎ 20.1001.1.23831367.1395.3.1.4.3

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Karimbeigi H, Nazarian-Firouzabadi F, Khademi M, Mousav E. Assessment of Genetic Diversity Among some Oilseed Rape (Brassica nupus L.) Plants, using Single Sequence Repeats (SSR) Molecular Markers. pgr 2016; 3 (1) :45-56
URL: http://pgr.lu.ac.ir/article-1-88-en.html

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