Genetic Diversity and Structure Analysis of Oily Sunflower (Helianthus annuus
L.) Based on Microsatellit Markers

Sahranavard Azartamar, Fatemeh; Ghadimzadeh, Mortaza; Darvishzadeh, Reza

doi:10.29252/pgr.2.2.15

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Volume 2, Issue 2 (2016)

pgr 2016, 2(2): 15-32

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Genetic Diversity and Structure Analysis of Oily Sunflower (Helianthus annuus L.) Based on Microsatellit Markers

Fatemeh Sahranavard Azartamar

, Mortaza Ghadimzadeh

, Reza Darvishzadeh ^*

Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran

Abstract: (24483 Views)

Knowledge about the amount of genetic diversity and understanding relationship between genotypes are important steps in plant germplasm conservation and breeding activities. In this study, the genetic diversity among 106 sunflower lines was assessed by 30 microsatellite primers. A total of 71 alleles were detected. Number of alleles in microssatellite loci ranged from 2 to 4 with the average number of 2.207 alleles per locus. The effective number of alleles ranged from 1.058 in locus ORS718 to 3.147 in locus HA3040. The average number of effective alleles was 1.641. The mean of PIC value was 0.344. Based on allele number and PIC value, SSR loci such as HA3040 and ORS733 are considered appropriate markers for studying genetic diversity in oily sunflower. Based on the results of cluster analysis using Jaccard's similarity coefficient and complete algorithm, the lines were grouped into four groups. Nineteen six out of 106 genotypes were grouped according to their origins (research centers). The highest and lowest Nei genetic distances were 0.21 and 0.004 between “NOVARTIS and HUNGARY” and “SPII with ENSAT and INRA-MONTPOL” groups, respectively. Analysis of the population structure revealed 5 subpopulations in the studied panel. The results show that the assignment of lines to subpopulations is not concordance with their geographical distribution pattern. The genetic diversity and distance revealed by SSR markers can be used in oily sunflower crossing and breeding programs

Keywords: Sunflower, Cluster analysis, Genetic diversity, Microsatellites, Subpopulation.

Full-Text [PDF 1169 kb] (3869 Downloads)

Type of Study: Research | Subject: Plant improvement

References

1. Basirnia, A., Darvishzadeh, R. and Abdollahi Mandoulakani, B. (2016). Retrotransposoninsertional polymorphism in sunflower (Helianthus annuus L.) lines revealed by IRAP andREMAP markers. Plant Biosystems, 150(4): 641-652.

2. Cheres, M.T. and Knapp, S.J. (1998). Ancestral origins and genetic diversity of cultivatedsunflower: Coancestry analysis of public germplasm. Crop Science, 38: 1476-1482.

3. Darvishzadeh, R. (2012). Phenotypic and molecular marker distance as a tool for prediction ofheterosis and F1 performance in sunflower (Helianthus annuus L.) under well-water and waterstressed conditions. Australian Journal of Crop Science, 6(4): 732-738.

4. Darvishzadeh, R., Azizi, M., Hatami-Maleli, H., Bernusi, I., Abdollahi Mandoulakani, B., Jafari, M. and Sarrafi, A. (2010). Molecular characterization and similarity relationships amongsunflower (Helianthus annuus L.) inbred lines using some simple sequence repeats. AfricanJournal of Biotechnology, 9: 7280-7288.

5. Dellaporta, S.L., Wood, J. and Hicks, J.B. (1983). A plant DNA mini-preparation: version II. PlantMolecular Biology Reporter, 1: 19-21.

6. Diz, P.A. and Presa, P. (2009). The genetic diversity pattern of Mytilus alloprovincialis in GalicianRías (NW Iberian estuaries). Aquaculture, 287: 278-285.

7. Dong, G.J., Liu, G.S. and Li, K.F. (2007). Studying genetic diversity in the core germplasm ofconfection sunflower (Helianthus annuus L.) in China based on AFLP and morphological analysis. Russian Journal of Genetics, 43: 627-635.

8. Dry, P.J. and Burdon, J.J. (1986). Genetic structure of natural populations of wild sunflowers (Helianthus annuus L.) in Australia. Australian Journal of Biological Science, 39: 255–270.

9. Eathing, S.R., Crosbie, T.M., Edwards, M.D., Reiter, R.S. and Bull, J.K. (2007). Molecularmarkers in a commercial breeding program. Crop Science, 47(S3): S154-S163.

10. Ebrahimi, M. and Zinoabedini, M. (2013). Application of genomic and unigene-based microsatellitemarkers in conservation and management of genetic resources of some Iranian crops. CropBiotechnology, 4: 133-148 (In Persian).

11. Evanno, G., Regnaut, S. and Goudet, J. (2005). Detecting the number of clusters of individualsusing the software STRUCTURE: a simulation study. Molecular Ecology, 14(8): 2620.

12. Fick G.N. and Zimmer, D.E. (1974). Parental lines for production of confection sunflower hybrids. North Dakota Farm Research, 31: 15-16.

13. Fick, G.N. (1989). Sunflower. pp. 301-318, In: G. Robbelen, R.K. Downey and A. Ashri (ed.), OilCrops of the world. McGraw-Hill, New York, USA.

14. Gabor, T., Gasper, Z. and Jurka, J. (2000). Microsatellite in different eukriotic genomes: surveyand analysis. Genome Research, 10: 967-981.

15. Galaev, A.V., Babayants, L.T. and Sivolap Yu., M. (2006). DNA-markers for resistance to commonbunt transferred from Aegilops cylindrica host to hexaploid wheat. Czech Journal of Genetics andPlant Breeding, 42: 62-65.

16. Garayalde, A.F., Poverene, M., Cantamutto, M. and Carrera, A.D. (2011). Wild sunflowerdiversity in Argentina revealed by ISSR and SSR markers: an approach for conservation andbreeding programmes. Annals of Applied Biology, 158: 305-317.

17. Gharghani, A., Zamani, Z., Talaie, A., Oraguzie, N.C., Fatahi, R., Hajnajari, H., Wiedow, C. and Gardiner, S.E. (2009). Genetic identity and relationships of Iranian apple (Malus ×domestica) cultivars and landraces, wild Malus species and representative old apple cultivars basedon simple sequence repeats (SSR) marker analysis. Genetic Resources and Crop Evolution, 13: 114.

18. Heiser, C.B. (1976). Sunflowers: Helianthus (Compositae- Heliantheae). pp. 36-38, In: N.W. Simmonds (ed.), Evolution of Crop Plants. Longmans Green, London, EN.

19. Hu, J., Seiler, G. and Kole, C. (2010). Genetics, Genomics and Breeding of Sunflower. CRC Press, Clemson, USA.

20. Iqbal, M.A., Sadaqat, H.A. and Khan, I.A. (2008). Estimation of genetic diversity among sunflowergenotypes through random amplified polymorphic DNA analysis. Genetics and MolecularResearch, 7: 1408-1413.

21. Kalia, R.K., Rai, M.K., Kalia, S., Singh, R. and Dhawan, A.K. (2011). Microsatellite markers: anoverview of the recent progress in plants. Euphytica, 177: 309-334.

22. Khelestkina E.K., Pestsova, E.G., Salina, E., Roder, M.S., Abruzova, V.S., Koval, S.F. andBomer, A. (2002). Genetic mapping and tagging of wheat genes using RAPD, STS and SSRmarkers. Cellular and Molecular Biology Letters, 7: 795-802.

23. Kholghi M., Darvishzadeh, R., Bernousi, I., Pirzad, A. and Laurentin, H. (2012). Assessment ofgenomic diversity among and within Iranian confectionary sunflower (Helianthus annuus L.) populations by using simple sequence repeat markers. Acta Agriculturae Scandinavica, Section B -Soil & Plant Science, 62: 488-498.

24. Kubo, N., Hirai, M., Kaneko, A., Tanaka, D. and Kasumi, K. (2009). Development and characterization of simple sequence repeat (SSR) markers in the water lotus (Nelumbo nucifera). Aquatic Botany, 90: 191-194.

25. Laurentin H. (2009). Data analysis for molecular characterization of plant genetic resources. GeneticResources and Crop Evolution, 56: 277-292.

26. Li Y.C., Korol, A.B., Fahima, T. and Nevo, E. (2004). Microsatellites within genes: structure, function, and evolution. Molecular Biology and Evolution, 21: 991-1007.

27. Liu, Z. (2007). Aquaculture Genome Technologies. Blackwell Publishing. Morgante, M., Hanafey, M. and Powell, W. (2002). Microsatellites are preferentially associatedwith nonrepetitive DNA in plant genomes. Nature Genetics, 30: 144-200.

28. Mondini, L., Noorani, A. and Pagnotta, M.A. (2009). Assessing plant genetic diversity by moleculartools. Diversity, 1: 19-35.

29. Muller, M.E., Delieux, F., Fernandez Martınez, J.M., Garric, B., Lecomte, V., Anglade, G., Leflon, M., Motard, C. and Segura, R. (2009). Occurrence, distribution and distinctivemorphological traits of weedy Helianthus annuus L. populations in Spain and France. GeneticsResources and Crop Evolution, 56: 869-877.

30. Naseri, L., Darvishzadeh, R., Mohseniazar, M. and Alizadeh, A. (2011). Molecularcharacterization and similarity relationships among some Iranian native and commercial apple (Malus × domestica) cultivars using simple sequence repeat markers. Journal of HorticultureScience and Biotechnology, 86(5): 527-533.

31. Nei, M. (1978). Estimation of average heterozygosity and genetic distance from a small number ofindividuals. Genetics, 89: 583-590.

32. Paniego N., Muñoz, M., Echaide, M., Fernandez, L., Faccio, P., Zandomeni, R., Suarez, E. andEsteban Hopp, H. (1999). Microsatellite development for sunflower. In: Proceeding of Plant andAnimal Genome VII Conference, San Diego: Starford University Press, USA.

33. Paniego, N., Echaide, M., Munoz, M., Fernandez, L., Torales, S., Faccio, P., Fuxan, I., Crrera, M., Zandomeni, R., Syarez E.Y. and Esteban Hopp H. (2002). Microsatellite isolation andcharacterization in sunflower (Helianthus annuus L.). Genome, 45: 34-43.

34. Paniego, N., Heinz, R., Fernandez, P., Talia, P., Nishinakamasu, V. and Esteban Hopp H. (2007). Sunflower. pp. 153-177. In: C. Kole (ed.), Genome Mapping and Molecular Breeding in PlantsVol. 2, Oilseeds. Springer-Verlag, Berlin-Heidelberg, Germany.

35. Peakall, R. and Smouse, P.E. (2006). GenAlEx 6: Genetic Analysis in Excel. Population geneticsoftware for teaching and research. Molecular Ecology Notes, 6: 288-295.

36. Piñera, J.A., Blanco, G., Vázquez, E. and Sánchez, J.A. (2007). Genetic diversity of black spot seabream (Pagellus bogaraveo) populations off Spanish Coasts: a preliminary study. Marine Biology, 151: 2153–2158.

37. Poormohammad Kiani, S., Talia, P., Maury, P., Grieu, P., Heinz, R., Perrault, A., Nishinakamasu, V., Hopp, E., Gentzbittel, L., Paniego, N. and Sarrafi, A. (2007). Geneticanalysis of plant water status and osmotic adjustment in recombinant inbred lines of sunflowerunder two water treatments. Plant Science, 172: 773-787.

38. Pritchard J.K., Stephens, M. and Donnelly, P. (2000). Inference of population structure usingmultilocus genotype data. Genetics, 155: 945-959.

39. Rincon, F., Johnson, B., Crossa, J. and Taba, S. (1996). Cluster analysis, an approach to samplingvariability in maize accessions. Maydica, 41: 307-316.

40. Rohlf F.J. (1998). NTSYSpc: Numerical Taxonomy and Multivariate Analysis System. Version 2.02.Exeter Software, Setauket, New York.

41. Rosenberg N.A., Pritchard, J.K., Weber, J.L., Cann, H.M., Kidd, K.K., Zhivotovsky, L.A. andFeldman, M.W. (2002). The genetic structure of human populations. Science, 298: 2381–2385.

42. Sabzalian M.R., Mirlohi, A.F., Saeidi, G. and Rabbani, M.T. (2009). Genetic variation amongpopulations of wild safflower, Carthamus oxyacanthus, analyzed by agro-morphological traits andISSR markers. Genetic Resource and Crop Evolution, 56: 1057-1064.

43. Tang, S. and Knapp S. J. (2003). Microsatellites uncover extraordinary diversity in native Americanlandraces and wild populations of cultivated sunflower. Theoretical and Applied Genetics, 106(6): 990-1003.

44. Tang, S., Yu, J.K., Slabaagh, M.B., Shintani, D.K. and Knapp, S.J. (2002). Simple sequencerepeats map of the sunflower genome. Theoretical and Applied Genetics, 105: 1124-1136.

45. Thormann, C.E., Ferreira, M.E., Camargo, L.E.A., Tivang, J.G. and Osborn, T.C. (1994). Comparison of RFLP and RAPD markers to estimating genetic relationships within and amongcruciferous species. Theoretical and Applied Genetics, 88: 973-980.

46. Varshney R.K., Graner, A. and Sorrells, M.E. (2005). Genic microsatellite markers in plants: features and applications. Trends in Biotechnology, 23: 48-55.

47. Vienne, D. (2003). Molecular Markers in Plants Genetics and Biotechnology. Science publishers Inc., Enfield, NH.

48. Weber, J.J. (1990). Informativeness of human (dC-dA) n (dG.dT)n polymorphisms. Genomics, 7: 524-530.

49. Wright, S. (1951). The genetical structure of populations. Annals of Eugenics, 15: 323-354.

50. Yu, J.K., Mangor, J., Thompson, L., Edwards, K.J., Slabaugh, M.B. and Knapp, S.J. (2002). Allelic diversity of simple sequence repeats among elite inbred lines of cultivated sunflower. Genome, 45: 652-660.

51. Zhang, L.S., Le Clerc, V., Li, S. and Zhang, D. (2005). Establishment of an effective set of simple sequence repeat markers for sunflower variety identification and diversity assessment. Canadian Journal of Botany, 83(1): 66-72.

‎ 10.29252/pgr.2.2.15

‎ 20.1001.1.23831367.1394.2.2.2.6

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Sahranavard Azartamar F, Ghadimzadeh M, Darvishzadeh R. Genetic Diversity and Structure Analysis of Oily Sunflower (Helianthus annuus L.) Based on Microsatellit Markers. pgr 2016; 2 (2) :15-32
URL: http://pgr.lu.ac.ir/article-1-45-en.html

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