[Home ] [Archive]   [ فارسی ]  
:: About :: Main :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..



 
..
:: Volume 1, Issue 2 (2015) ::
pgr 2015, 1(2): 71-78 Back to browse issues page
Quantitative Detection of Transgenic Roundup Ready Soybean Seeds Using Real-time PCR Method
Elham Ghazizadeh , Amir Mousavi * , Faranak Hadi
Associate Professor, Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran , m-amir@nigeb.ac.ir
Abstract:   (19684 Views)
As genetically modified organisms (GMOs) development is now increasing, detection and determination of their quantitative threshold using reliable methods would be necessary. The goal of this study was to introduce a sensitive method for qualitative and quantitative detection of Roundup-ready soybean samples. For primary screening, semi-quantitative molecular assays have been used for detection of various percentages of transgenic and non-transgenic Roundup-ready soybean samples. Furthermore, an experiment was conducted using the CaMV 35S primers in combinations with soybean lectin-specific primers in two imported samples of soybean seeds. Real-time PCR-based analysis indicated that the amount of GMO material in the seeds and the limit of detection (LOD) obtained for 35S sequence was less than 1%. The sensitivity and accuracy of this method had conformity with the international standards of seed labeling. This is the first report of its type for quantitative detection of a genetically modified material in a commercial seed lot in Iran.
Keywords: Limit of detection, Genetically modified crop, Roundup ready soybean
Full-Text [PDF 473 kb]   (2867 Downloads)    
Type of Study: Research | Subject: Plant improvement
References
1. Bergerova, E., Godalova, Z. and Siekel, P. (2011). Combined effect of temperature, pressure and low pH on the DNA amplification of plant derived foods. Czech Journal of Food Sciences, 29: 345-337.
2. Corbisier, P., Trapmann, S., Gancberg, D., Hannes, L., Van Iwaarden, P., Berben, G.,Schimmel, H. and Emons, H. (2005). Quantitative determination of Roundup Ready soybean (Glycine max) extracted from highly processed flour. Analytical and Bioanalytical Chemistry, 383: 290-282.
3. Ghazizadeh, E., Mousavi, A., Hadi, F. and Hashemi Sohi, H. Detection of Transgenic Roundup Ready Soybean Seeds by Molecular Methods. Iranian Journal of Biology, In press.
4. Gurel, F., Arican, E., Gozukirmizi, N. and Ari, S., (2011). Recent molecular tools for detecting transgenic events in genetically modified (GM) crop products. Scientific Research and Essays, 6: 5099-5091.
5. Hodek, J., Ovesna, J. and Kucera, L. (2009). Interferences of PCR effectivity: importance for quantitative analyses. Czech Journal of Food Sciences, 27: 42-49.
6. Holst-Jensen, A., De Loose, M. and Van Den Eede, G. (2006). Coherence between legal requirements and approaches for detection of Genetically Modified Organisms (GMOs) and their derived products. Journal of Agricultural and Food Chemistry, 54: 2799–2809.
7. Ingham, D.J., Beer, S., Money, S. and Hansen, G. (2001). Quantitative Real-time PCR assay for determining transgene copy number in transformed plants. Biotechniques, 31: 132-4, 136-40.
8. James, C. (2013). Global Status of Commercialized Biotech/GM Crops: 2012. The International Service for the Acquisition of Agri-biotech Applications (ISAAA), Ithaca, NY.
9. Lin, H. Y., Chiueh, L. C. and Shih, D. Y. C. (2000). Detection of genetically modified soybeans and maize by the polymerase chain reaction method. Journal of Food and Drug Analysis, 8: 200-207.
10. Lipp, M., Brodmann, P., Pietsch, K., Pauwels, J. and Anklam, E. (1999). IUPAC collaborative trial study of a method to detect genetically modified soybeans and maize in dried powder. Journal of AOAC International, 82: 923-928.
11. Marmiroli, N., Maestri, E., Gulli, M., Malcevschi, A., Peano, C., Bordoni, R. and De Bellis, G. (2008). Methods for detection of GMOs in food and feed. Analytical and Bioanalytical Chemistry, 392: 348-369.
12. Murray, H.G. and Thompson, W.F. (1980). Rapid isolation of high molecular weight DNA. Nucleic Acids Research, 8: 4321-4325.
13. Ovesna, J., Kucera, L., Hodek, J. and Demnerova, K. (2010). Reliability of PCR based Screening for Identification and Quantification of GMOs. Czech Journal of Food Sciences, 28: 133-138.
14. Paoletti, C., Heissenberger, A., Mazzara, M., Larcher, S., Grazioli, E., Corbisier, P., Hess, N.,Berben, G., Lubeck, P.S., De Loose, M., Moran, G., Henry, Ch., Brera, C., Folch, I., Ovesna,J. and Van den Eede, G. (2006). Kernel lot distribution assessment (KeLDA): a study on the distribution of GMO in large soybean shipments. European Food Research and Technology, 224: 129-139.
15. Querci, M., Jeremini, M. and Van den Eede, G. (2006). The analysis of food samples for the presence of genetically modified organisms. User Manual. JRC.
16. Rabiei, M., Mehdizadeh, M., Rastegar, H., Vahidi, H. and Alebouyeh, M. (2013). Detection of Genetically Modified Maize in Processed Foods Sold Commercially in Iran by Qualitative PCR. Iranian Journal of Pharmaceutical Research, 12: 25-30
17. Spoth, B. and Strauss, E. (1998). Screening for genetically modified organisms in food using, Promega’s Wizard resin. Promega Notes, 73: 23-25.
18. Untergasser, A., Nijveen, H., Rao, X., Bisseling, T., Geurts, R. and Leunissen, J.A.M. (2007). Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Research, 35: 71-74.
19. Vrablik, A., Hodek, J., Soukup, J. and Demnerova, K. J. (2012). Development and Verification of PCR based Assay to Detect, and Quantify Garden Pea lec Gene, Czech Journal of Food Sciences, 30: 257-247.
20. Wang, W. and Fang, T. (2005). Development of Multiplex and Quantitative PCR Assay to Detect Genetically Modified Roundup Ready Soybean in Foods. Journal of Food and Drug Analysis, 13: 138-132.
Send email to the article author



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghazizadeh E, Mousavi A, Hadi F. Quantitative Detection of Transgenic Roundup Ready Soybean Seeds Using Real-time PCR Method. pgr 2015; 1 (2) :71-78
URL: http://pgr.lu.ac.ir/article-1-37-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 1, Issue 2 (2015) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
Persian site map - English site map - Created in 0.06 seconds with 38 queries by YEKTAWEB 4657