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Introducing Two Stripe Rust Resistance Genes, Yr5 and Yr15, to Some Iranian Bread Wheat Cultivars Using Marker-Assisted Backcrossing
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Fatemeh Bagherzadeh  , Hannaneh Mirahmadi , Soraya Pourtabrizi *  , Ali Kazemipour , Maryam Dorraninejad , Roohollah Abdoshahi |
| Department of Plant Production and Genetics, Shahid Bahonar University of Kerman, Kerman, Iran , s.pourtabrizi@empl.uk.ac.ir |
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Abstract: (120 Views) |
Stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases of wheat in many parts of the world. Genetic resistance is the best way to deal with this disease. This disease evolves very quickly, and for this reason, the resistance of many major-effect resistance genes has been overcome. In this study, the Yr5 and Yr15 genes, which are among the most effective resistance genes in a full-grown plant stage, were introduced to the Iranian cultivars, including Baharan, Rakhshan, Setareh, Sivand, Parsi, and Amin using the marker-assisted backcrossing method. These genes were intrroduced to the mentioned cultivars in six distinct breeding projects. Cultivars were crosses with the donor parents Yr5/6*Avocet‘S’and Yr15/6*Avocet‘S’. The progeny of F1 generation, were backcrossed with the Iranian cultivars (recurrent parent) to obtain BC1F1 progeny in each population. By genotyping 30 random plants in each project, the heterozygous genotypes carrying the resistance genes were identified using specific markers, and the second backcrossing was performed. Overall, the results of this studuy demonstrated that within each population, a stripe rust-resistant line can be developed through repeated generations of backcrossing followed by one generation of selfing. The introduction and pyramiding of yellow rust resistance genes into the Iranian cultivars not only enhance durable resistance and mitigate yield losses during epidemic years but also can contribute to benefits to the environment. |
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| Keywords: Back cross, Durable resistance, Major genes, Resistance genes, Specific marker |
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Type of Study: Applicable |
Subject:
Plant improvement
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| Send email to the article author |
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