The Effect of Iron Deficiency on the Relative Expression of Genes Encoding Catalase, Ascorbate Peroxidase and Polyphenol Oxidase Enzymes in Bread Wheat

Ranjbar, Fariba; Abdollahi Mandoulakani, Babak; Ghasemzadeh, Raheleh

doi:10.22034/pgr.10.1.9

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Volume 10, Issue 1 (2023)

pgr 2023, 10(1): 145-156

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The Effect of Iron Deficiency on the Relative Expression of Genes Encoding Catalase, Ascorbate Peroxidase and Polyphenol Oxidase Enzymes in Bread Wheat

Fariba Ranjbar

, Babak Abdollahi Mandoulakani ^*

, Raheleh Ghasemzadeh

Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran , b.abdollahi@urmia.ac.ir

Abstract: (1142 Views)

To evaluate the expression pattern of genes encoding antioxidant enzymes catalase, ascorbate peroxidase and polyphenol oxidase under iron deficiency conditions in Fe- efficient (Pishtaz) and -inefficient (Falat) bread wheat cultivars, a CRD (completely randomized design) based factorial experiment was conducted with three replications. The cultivars were grown under iron deficiency (Less than 1.5 mg Fe/kg soil) and compared with normal conditions (10 mg Fe/kg soil). The relative expression levels of the above-mentioned genes were measured using Real-time PCR technique in the leaves and roots of the cultivars at two growth stages: vegetative (one month after germination) and reproductive (30% of heading). The results revealed a remarkable enhancement in calatalse expression in the roots of both cultivars in the vegetatative stage but it was higher in Fe-efficient cultivar than -inefficient one. The expression of this gene was decreased in leaves at the same stage as well as in the roots of both cultivars in the vegetative stage. The expression level of ascorbate peroxidase gene in the reproductive stage in the roots of Fe-inefficient cultivar was higher than that of -efficient one. In the vegetative stage, the expression of this gene increased in the leaves and roots of Fe-efficient cultivar, but it was decresed in Fe-inefficient cultivar. The relative expression level of polyphenol oxidase gene in the vegetative stage under iron deficiency conditions in the leaf increased almost three times, compared to the roots, while the expression of this gene decreased in the reproductive stage in both leaves and roots. By increasing the expression of both catalase and ascorbate peroxidase genes in the roots of both cultivars in the reproductive stage under iron deficiency conditions, it seems that bread wheat cultivars might reduce the deletrious effects of stress and maintain yield through transferring much iron to the seeds in the seed filling stage. The findings of the present study may increase our understanding of the important role of genes encoding antioxidant enzymes in Fe deficiency stress conditions.

Keywords: Antioxidant enzymes, Fe deficiency, Gene expression, Wheat

Full-Text [PDF 1322 kb] (255 Downloads)

Type of Study: Research | Subject: Molecular genetics
Accepted: 2023/07/26

References

1. Abdoli, M. and Esfandiari, E. (2014). Effect of zinc foliar application on the quantitative and qualitative yield and seedlings growth characteristics of bread wheat (cv. Kohdasht). Iranian Journal of Dryland Agriculture, 3: 77-90 (In Persian).

2. Agarwal, S., Sairam, R.K., Meena, R.C., Tyagi, A. and Srivastava, G.C. (2006). Effect of excess and deficient levels of iron and copper on oxidative stress and antioxidant enzymes activity in wheat. Plant Science, 1: 86-97.

3. Assimakopoulou, A., Holevas, C.D. and Fasseas, K. (2011). Relative susceptibility of some Prunus rootstocks in hydroponics to iron deficiency. Journal of Plant Nutrition, 34: 1014-1033. [DOI:10.1080/01904167.2011.555583]

4. Boudet, A.M. (2000). Lignins and lignification: selected issues. Plant Physiology and Biochemistry, 38: 81-96. [DOI:10.1016/S0981-9428(00)00166-2]

5. Dinakar, N., Nagajyothi, P.C., Suresh, S., Udaykiran, Y. and Damodharam, T. (2008). Phytotoxicity of cadmium on protein, proline and antioxidant enzyme activities in growing Arachis hypogaea seedlings. Journal of Environmental Sciences, 20: 199-206. [DOI:10.1016/S1001-0742(08)60032-7]

6. Fahmideh, L., Delarampoor, M.A. and Fooladvand, Z. (2020). Study of MYB transcription factor gene expression in some bread wheat cultivars of Sistan region, Iran. Plant Genetic Researches, 7(1): 181-196 (In Persian). [DOI:10.52547/pgr.7.1.11]

7. FAO. (2019). FAOSTAT. Food and Agricultural Organization of the United Nations. Available at http://www.fao.org/faostat/en/#data/QC (accessed on 9 September 2019). FAO. Rome, Italy.

8. Gogorcena, Y., Abadía, J. and Abadía, A. (2005). A new technique for screening iron-efficient genotypes in peach rootstocks: elicitation of root ferric chelate reductase by manipulation of external iron concentrations. Journal of Plant Nutrition, 27: 1701-1715. [DOI:10.1081/PLN-200026406]

9. Gupta, B., Pathak, G.C. and Pandey, N. (2011). Induction of oxidative stress and antioxidant responses in Vigna mungo by zinc stress. Journal of Plant Physiology, 58: 85-91. [DOI:10.1134/S1021443711010079]

10. Hao, C., Wang, Y., Chao, S., Li, T., Liu, H., Wang, L. and Zhang, X. (2017). The iSelect 9 K SNP analysis revealed polyploidization induced revolutionary changes and intense human selection causing strong haplotype blocks in wheat. Scientific Reports, 7(1): 41247. [DOI:10.1038/srep41247]

11. Hasheminasab, F.S., Mousavi Baygi, M., Bakhtiari, B. and Bannayan Aval, M. (2014). The effects of rainfall on dryland wheat yield and water requirement satisfaction index at different time scales. Journal of Irrigation and Water Engineering, 5: 1-13 (In Persian).

12. Hossain, A., da Silva, J.A.T., Lozovskaya, M.V., Zvolinsky, V.P. and Mukhortov, V.I. (2012). High temperature combined with drought affect rainfed spring wheat and barley in South-Eastern Russia: I. Phenology and growth. Saudi Journal of Biological Sciences, 4: 184-196. [DOI:10.1016/j.sjbs.2012.07.005]

13. Kaur, N.P., Takkar, P.N. and Nayyar, V.K. (1984). Catalase, peroxidase, and chlorphyll relationships to yield and iron deficiency chlorosis in Cicer genotypes. Journal of Plant Nutrition, 7: 1213-1220. [DOI:10.1080/01904168409363276]

14. Kiani Chalmardi, Z., Abdolzadeh, A. and Sadeghipour, H.R. (2013). Evaluation of the effects of silicon nutrition on alleviation of iron deficiency in rice plants (Oryza sativa L.) with emphasis on growth and antioxidant enzymes activity. Iranian Journal of Plant Biology, 4: 61-74 (In Persian).

15. Malakuti, M.J. and Tehrani, M.M. (2005). The Role of Micronutrients in Increasing the Yield and Improving the Quality of Macronutrient Microelements Products. Publications of Tarbiat Modares University, Tehran, IR (In Persian).

16. Mirparizi, E., Barani Motlagh, M., Movahedi Naeini, S.A., Ghorbani Nasrabadi, R., and Bakhtiary, S. (2022). Effects of copper slag and organic matters on physiological indices of iron deficiency in sorghum (Sorghum bicolor). Journal of Water and Soil, 35(6): 823-841 (In Persian).

17. Molassiotis, A., Tanou, G., Diamantidis, G., Patakas, A. and Therios, I. (2006). Effects of 4-month Fe deficiency exposure on Fe reduction mechanism, photosynthetic gas exchange, chlorophyll fluorescence and antioxidant defense in two peach rootstocks differing in Fe deficiency tolerance. Journal of Plant Physiology, 163: 176-185. [DOI:10.1016/j.jplph.2004.11.016]

18. Munns, R. and Tester, M. (2008). Mechanisms of salinity tolerance. Annual review of Plant Biology, 59: 651-681. [DOI:10.1146/annurev.arplant.59.032607.092911]

19. Narimani, H., Seyed Sharifi, R., Khalilzadeh, R. and Aminzadeh, G. (2018 ). Effects of nano iron oxide on yield, chlorophyll fluorescence indices and some physiological traits of wheat (Triticum aestivum L.) under rain fed and supplementary irrigation conditions. Iranian Journal of Plant Biology, 10(3): 21-40 (In Persian).

20. Nasiri, Y., Zehtab Salmasi, S., Nasrullah Zadeh, S., Ghassemi Gholezani, K., Najafi, N. and Javanmard, A. (2013). Evaluation of foliar spray of ferrous sulfate and zinc sulfate on yield and nutrients concentration of aerial parts in German chamomile. Journal of Agricultural Science and Sustainable Production, 23: 105-115 (In Persian).

21. Nenova, V. and Stoyano, I. (1995). Physiological and biochemical changes in young maize plants under iron deficiency: 2. Catalase, peroxidase, and nitrate reductase activities in leaves. Journal of Plant Nutrition, 18: 2081-2091. [DOI:10.1080/01904169509365046]

22. Niazkhani, S.M., Abdollahi Mandoulakani, B., Jafari, M. and Rasouli-Sadaghiani, M.H. (2018). Effect of soil zinc deficiency on antioxidant enzymes activity and some biochemical parameters in bread wheat. Scientific Journal of Crop Physiology, 11: 5-27 (In Persian).

23. Phua, S.Y., De Smet, B., Remacle, C., Chan, K.X. and Van Breusegem, F. (2021). Reactive oxygen species and organellar signaling. Journal of Experimental Botany, 72(16): 5807-5824. [DOI:10.1093/jxb/erab218]

24. Pirzad, A.R., Tousi, P. and Darvishzadeh, R. (2013). Effect of Fe and Zn foliar application on plant characteristics and essential oil content of anise (Pimpinella anisum L.). Iranian Journal of Crop Sciences. 15: 12-23 (In Persian).

25. Raeesi Sadati, S.Y., Jahanbakhsh Godehkahriz, S., Ebadi, A. and Sedghi, M. (2021). Study of expression pattern of some transcription factors in wheat under drought stress and zinc nanoparticles. Plant Genetic Researches, 7(2): 135-144 (In Persian). [DOI:10.52547/pgr.7.2.11]

26. Rahimi Jarihani, L. and Abdollahi Mandoulakani, B. (2019). Expression pattern of genes encoding catalase, ascorbate peroxidase and polyphenol oxidase enzymes under conditions of soil zinc deficiency in bread wheat. Journal of Cell and Molecular Research, 34: 1-12 (In Persian).

27. Rajput, V., Minkina, T., Yaning, C., Sushkova, S., Chapligin, V. and Mandzhieva, S. (2016). A review on salinity adaptation mechanism and characteristics of Populus euphratica, a boon for arid ecosystems. Acta Ecologica Sinica, 36: 497-503. [DOI:10.1016/j.chnaes.2016.08.001]

28. Reddy, K.R. and Hodges, H.F. (2000). Climate Change and Global Crop Productivity. CABI, Wallingford, UK. [DOI:10.1079/9780851994390.0007]

29. Shi, X., Su-nan, X., Yue, L. and Zhong-lin, C.H. (2013). Impacts of zinc, benzo [a] pyrene, and their combination on the growth and antioxidant enzymes activities of wheat (Triticum aestivum L.) seedlings. Journal of Ecology, 32: 358-362.

30. Tiryakioglu, M., Eker, S., Ozkutlu, F., Husted, S. and Cakmak, I. (2006). Antioxidant defense system and cadmium uptake in barley genotypes differing in cadmium tolerance. Journal of Trace Elements in Medicine and Biology, 20: 181-189. [DOI:10.1016/j.jtemb.2005.12.004]

31. Von der Mark, C., Ivanov, R., Eutebach, M., Maurino, V.G., Bauer, P. and Brumbarova, T. (2021). Reactive oxygen species coordinate the transcriptional responses to iron availability in Arabidopsis. Journal of Experimental Botany, 72: 2181-2195. [DOI:10.1093/jxb/eraa522]

32. Wang, M., Kawakami, Y. and Bhullar, N.K. (2019). Molecular analysis of iron deficiency response in hexaploid wheat. Frontiers in Sustainable Food Systems, 3: 67.‌ [DOI:10.3389/fsufs.2019.00067]

33. Wani, S.H. and Sah, S.K. (2014). Biotechnology and abiotic stress tolerance in rice. Journal of Rice Research, 2(2): e105. [DOI:10.4172/jrr.1000e105]

34. Winfield, M.O., Allen, A.M., Wilkinson, P.A., Burridge, A.J., Barker, G.L., Coghill, J., Waterfall, C., Wingen, L.U., Griffiths, S. and Edwards, K.J. (2018). High‐density genotyping of the AE Watkins Collection of hexaploid landraces identifies a large molecular diversity compared to elite bread wheat. Plant Biotechnology Journal, 16(1): 165-175. [DOI:10.1111/pbi.12757]

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Ranjbar F, Abdollahi Mandoulakani B, Ghasemzadeh R. The Effect of Iron Deficiency on the Relative Expression of Genes Encoding Catalase, Ascorbate Peroxidase and Polyphenol Oxidase Enzymes in Bread Wheat. pgr 2023; 10 (1) :145-156
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