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:: Volume 9, Issue 1 (2022) ::
pgr 2022, 9(1): 43-56 Back to browse issues page
Evaluation of Some Characteristics of Substantial Equivalence of a Salinity-Resistant Transgenic Potato
Samira Karimi , Maghsoud Pazhouhandeh * , Kambiz Azizpour
Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran , pazhouhandeh@azaruniv.edu
Abstract:   (3594 Views)
Transgenic plants and their products are being developed day by day due to their improved characteristics, and it is necessary to evaluate the safety of these plants before releasing them. Hence, the importance of the issue of biosafety of transgenic plants and the use of their products has led the regulatory agencies to create some laws called substantial equivalence. based on that, the essential nutrients of transgenic plants are examined and compared with the control. The present study aimed to compare the transgenic potato line F (salinity-resistant) with non-transgenic Agria cultivar plants. The salt resistant potato line was produced by transferring Arabidopsis SOS3 gene to potato (Agria variety) and its resistance was confirmed. First, the presence of AtSOS3 gene in F-line plants was confirmed and then the substantial equivalent experiments were performed by comparing the production of proline, soluble sugars, carotenoids and chlorophylls a and b, the relative expression of Catalase1 (CAT1) and AtSOS3 gene between F and non-transgenic WT Agria plants. Based on evaluations of physiological traits and some metabolites (proline content, soluble sugars, carotenoids and chlorophylls a and b) and morphological traits (plant height, dry and fresh weight of plant) between line F and WT, no significant difference was observed. The number of microbiome colonies around the root in the transgenic F and non-transgenic WT plants was a non-significant difference, which indicates that the transgenic line has no threatening effects on the environment and human pathogenicity. The relative expression of AtSOS3 and Catalase1 genes in line F had higher values than WT. The reason for such increase in the expression of Catalase1 is the activation of plant defense mechanisms against stress. Finally, the results of the evaluations proved the equality of line F and WT
Keywords: Substantial equivalence, Transgenic, Potato, Salinity, Resistance
Full-Text [PDF 573 kb]   (1176 Downloads)    
Type of Study: Research | Subject: Plant improvement
References
1. Ahanger, M.A., Akram, N.A., Ashraf, M., Alyemeni, M.N., Wijaya, L. and Ahmad, P. (2017). Plant responses to environmental stresses-from gene to biotechnology. AoB Plants, 9(4): plx025. [DOI:10.1093/aobpla/plx025]
2. Akhtar, A., Rizvi, Z., Irfan, M., Maqbool, A., Bashir, A. and Malik, K.A. (2020). Biochemical and morphological risk assessment of transgenic wheat with enhanced iron and zinc bioaccessibility. Journal of Cereal Science, 91: 102881. [DOI:10.1016/j.jcs.2019.102881]
3. Anirudh, K.V.S., Chakraborty, T., Srivastava, R.K. and Akhtar, N. (2020). Effect of Drought and Salt Stress On Cereal Crop Plants and Their Proteomic and Physiological Studies. Journal of Biotechnology and Biomedical Science, 2: 43. [DOI:10.14302/issn.2576-6694.jbbs-20-3525]
4. Azooz, M., Ismail, A. and Elhamd, M.A. (2009). Growth, lipid peroxidation and antioxidant enzyme activities as a selection criterion for the salt tolerance of maize cultivars grown under salinity stress. International Journal of Agriculture and Biology, 11: 21-26.
5. Bedair, M. and Glenn, K.C. (2020). Evaluation of the use of untargeted metabolomics in the safety assessment of genetically modified crops. Metabolomics, 16: 1-15. [DOI:10.1007/s11306-020-01733-8]
6. Chen, B.C., Lin, H.Y., Chen, J.T., Chao, M.L., Lin, H.T. and Chu, W.S. (2020). Compositional Analysis of the Transgenic Potato with High-level Phytase Expression. Journal of Food and Nutrition Research, 8: 231-237.
7. Fedina, I., Georgieva, K., Velitchkova, M. and Grigorova, I. (2006). Effect of pretreatment of barley seedlings with different salts on the level of UV-B induced and UV-B absorbing compounds. Environmental and Experimental Botany, 56: 225-230. [DOI:10.1016/j.envexpbot.2005.02.006]
8. Fedorova, M. and Herman, R.A. (2020). Obligatory metabolomic profiling of gene‐edited crops is risk disproportionate. The Plant Journal, 103: 1985-1988. [DOI:10.1111/tpj.14896]
9. Ghazizadeh, E., Mousavi, A. and Hadi, F. (2015). Quantitative detection of transgenic roundup ready soybean seeds using real-time PCR method. Plant Genetic Researches, 1: 71-78 (In Persian). [DOI:10.29252/pgr.1.2.71]
10. Giraldo, P.A., Shinozuka, H., Spangenberg, G.C., Cogan, N.O. and Smith, K.F. (2019). Safety assessment of genetically modified feed: is there any difference from food? Frontiers in Plant Science, 10: 1592. [DOI:10.3389/fpls.2019.01592]
11. Gong, D., Guo, Y., Schumaker, K.S. and Zhu, J.K. (2004). The SOS3 family of calcium sensors and SOS2 family of protein kinases in Arabidopsis. Plant Physiology, 134: 919-926. [DOI:10.1104/pp.103.037440]
12. Gupta, U.C. and Gupta, S.C. (2019). The important role of potatoes, an underrated vegetable food crop in human health and nutrition. Current Nutrition & Food Science, 15: 11-19. [DOI:10.2174/1573401314666180906113417]
13. Hilbeck, A., Meyer, H., Wynne, B. and Millstone, E. (2020). GMO regulations and their interpretation: how EFSA's guidance on risk assessments of GMOs is bound to fail. Environmental Sciences Europe, 32: 1-15. [DOI:10.1186/s12302-020-00325-6]
14. Hirt, H. (2020). Healthy soils for healthy plants for healthy humans: How beneficial microbes in the soil, food and gut are interconnected and how agriculture can contribute to human health. EMBO Reports, 21: e51069. [DOI:10.15252/embr.202051069]
15. Hong, B., Fisher, T.L., Sult, T.S., Maxwell, C.A., Mickelson, J.A., Kishino, H. and Locke, M.E. (2014). Model-based tolerance intervals derived from cumulative historical composition data: application for substantial equivalence assessment of a genetically modified crop. Journal of Agricultural and Food Chemistry, 62: 9916-9926. [DOI:10.1021/jf502158q]
16. Jiang, C., Meng, C., Schapaugh, A.W. and Jin, H. (2021). Comparative Analysis of Genetically-Modified Crops: Conditional Equivalence Criteria. bioRxiv, https://doi.org/10.1101/2021.02.19.431950 [DOI:10.1101/2021.02.19.431950.]
17. Khalf, M., Goulet, C., Vorster, J., Brunelle, F., Anguenot, R., Fliss, I. and Michaud, D. (2010). Tubers from potato lines expressing a tomato Kunitz protease inhibitor are substantially equivalent to parental and transgenic controls. Plant Biotechnology Journal, 8: 155-169. [DOI:10.1111/j.1467-7652.2009.00471.x]
18. Kim, E.H., Oh, S.W., Lee, S.Y., Park, H.Y., Kang, Y.Y., Lee, K.M., Baek, D.Y., Kang, H.J., Park, S.Y. and Ryu, T.H. (2020). Comparison of the Seed Nutritional Composition between Conventional Varieties and Transgenic Soybean Overexpressing Physaria FAD3‐1. Journal of the Science of Food and Agriculture. 101: 2601-2613. [DOI:10.1002/jsfa.11028]
19. Kim, J.K., Park, S.Y., Lee, S.M., Lim, S.H., Kim, H.J., Oh, S.D., Yeo, Y., Cho, H.S. and Ha, S.H. (2013). Unintended polar metabolite profiling of carotenoid-biofortified transgenic rice reveals substantial equivalence to its non-transgenic counterpart. Plant Biotechnology Reports, 7: 121-128. [DOI:10.1007/s11816-012-0231-6]
20. Kok, E.J. and Kuiper, H.A. (2003). Comparative safety assessment for biotech crops. TRENDS in Biotechnology, 21: 439-444. [DOI:10.1016/j.tibtech.2003.08.003]
21. Koubaa, R.J., Ayadi, M., Saidi, M.N., Charfeddine, S., Bouzid, R.G. and Nouri-Ellouz, O. (2022). Comprehensive Genome-Wide Analysis of The Catalase Enzyme Toolbox In Potato (Solanum Tuberosum L.). Potato Research, doi.org/10.1007/s11540-022-09554-z [DOI:10.21203/rs.3.rs-1020759/v1]
22. Kour, D., Kaur, T., Devi, R., Rana, K.L., Yadav, N., Rastegari, A.A. and Yadav, A.N. (2020) Biotechnological applications of beneficial microbiomes for evergreen agriculture and human health. In: Rastegari, A.A., Yadav, A.N. and Yadav, N., Eds., New and Future Developments in Microbial Biotechnology and Bioengineering, pp. 255-279, Elsevier, Amsterdam, NL. [DOI:10.1016/B978-0-12-820528-0.00019-3]
23. Mahmoud, A.W.M., Abdeldaym, E.A., Abdelaziz, S.M., El-Sawy, M.B. and Mottaleb, S.A. (2020). Synergetic effects of zinc, boron, silicon, and zeolite nanoparticles on confer tolerance in potato plants subjected to salinity. Agronomy, 10(1): 19. [DOI:10.3390/agronomy10010019]
24. Maroušek, J., Rowland, Z., Valášková, K. and Král, P. (2020). Techno-economic assessment of potato waste management in developing economies. Clean Technologies and Environmental Policy, 22: 1-8. [DOI:10.1007/s10098-020-01835-w]
25. Mendes, R., Garbeva, P. and Raaijmakers, J.M. (2013). The rhizosphere microbiome: significance of plant beneficial, plant pathogenic, and human pathogenic microorganisms. FEMS Microbiology Reviews, 37: 634-663. [DOI:10.1111/1574-6976.12028]
26. Mirrokni, H., Rahnama, H. and Zeinali, H. (2014). Evaluation of total carbohydrate and soluble sugars in transgenic potato resistant to potato tuber moth. Genetic Engineering and Biosafety Journal, 3: 67-75.
27. Moatamed, E. (2016). The study on transformation of potato with AtSOS3 gene in order to establish resistance to salinity. M.Sc Thesis, Azarbaijan Shahid Madani University, Iran (In Persian).
28. Mosavi, M., Khorshidi, M., Masoudian, N. and Hokmabadi, H. (2018). Study of some physiological characteristics of potato tissue under salinity stress. International Journal of Farming and Allied Sciences, 7: 1-5.
29. Mukerji, P., Rudgers, G.W., Gibson, C. and Roper, J.M. (2020). Safety evaluation of E12, W8, X17, and Y9 potatoes: Nutritional evaluation and 90-day subchronic feeding study in rats. Regulatory Toxicology and Pharmacology, 115: 104712. [DOI:10.1016/j.yrtph.2020.104712]
30. Nowroz, F., Roy, T.S., Haque, M.T., Ferdous, J., Noor, R. and Mondal, G.C. (2021). Yield and grading of potato (Solanum tuberosum L.) as influenced by different mulch materials. Agrotechniques in Industrial Crops, 1: 1-10.
31. Peng, C., Ding, L., Hu, C., Chen, X., Wang, X., Xu, X., Li, Y. and Xu, J. (2019). Effect on metabolome of the grains of transgenic rice containing insecticidal cry and glyphosate tolerance epsps genes. Plant Growth Regulation, 88: 1-7. [DOI:10.1007/s10725-019-00482-6]
32. Prado, J.R., Segers, G., Voelker, T., Carson, D., Dobert, R., Phillips, J., Cook, K., Cornejo, C., Monken, J. and Grapes, L. (2014). Genetically engineered crops: from idea to product. Annual Review of Plant Biology, 65: 769-790. [DOI:10.1146/annurev-arplant-050213-040039]
33. Prochazkova, D., Sairam, R., Srivastava, G. and Singh, D. (2001). Oxidative stress and antioxidant activity as the basis of senescence in maize leaves. Plant Science, 161: 765-771. [DOI:10.1016/S0168-9452(01)00462-9]
34. Rahimian, M.H. and Zabihi, H.R. (2021). Investigation of the Reaction of Potato Plant to Magnetized Saline Water. Agrotechniques in Industrial Crops, 1: 149-153.
35. Rahnama, H., Moradi, A.B., Mirrokni, S.H., Moradi, F., Shams, M.R. and Fotokian, M.H. (2018). Comparative compositional analysis of transgenic potato resistant to potato tuber moth (PTM) and its non-transformed counterpart. Transgenic Research, 27: 301-313. [DOI:10.1007/s11248-018-0075-0]
36. Sairam, R.K., Rao, K.V. and Srivastava, G. (2002). Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science, 163: 1037-1046. [DOI:10.1016/S0168-9452(02)00278-9]
37. Salami, R., Mohammadi, S.A., Ghafarian, S. and Moghaddam, M. (2016). Expression analysis of Hv TIP2; 3 and Hv TIP4; 1 in sensitive and tolerant barley genotypes under salinity stress. Plant Genetic Researches, 2(2): 1-14 (In Persian). [DOI:10.29252/pgr.2.2.1]
38. Scott, G.J., Petsakos, A. and Juarez, H. (2019). Climate change, food security, and future scenarios for potato production in India to 2030. Food Security, 11: 43-56. [DOI:10.1007/s12571-019-00897-z]
39. Sevestre, F., Facon, M., Wattebled, F. and Szydlowski, N. (2020). Facilitating gene editing in potato: a Single-Nucleotide Polymorphism (SNP) map of the Solanum tuberosum L. cv. Desiree genome. Scientific Reports, 10: 1-8. [DOI:10.1038/s41598-020-58985-6]
40. Shabani, A., Zebarjadi, A., Mostafaei, A., Mohsen, S. and Poordad, S.S. (2016). Identification of drought stress responsive proteins in susceptible genotype of chickpea (Cicer arietinum L.). Plant Genetic Researches, 3(1): 1-12 (In Persian). [DOI:10.29252/pgr.3.1.1]
41. Sofo, A., Scopa, A., Nuzzaci, M. and Vitti, A. (2015). Ascorbate peroxidase and catalase activities and their genetic regulation in plants subjected to drought and salinity stresses. International Journal of Molecular Sciences, 16: 13561-13578. [DOI:10.3390/ijms160613561]
42. Sreekar, K. (2020). Biotechnology and its implications in brinjal improvement: A review. Journal of Pharmacognosy and Phytochemistry, 9: 1096-1102.
43. Valikhanlou, N. (2018). The Assessment of Resistance to Salinity in Transgenic Potato lines with AtSOS3 gene. M.Sc Thesis, Azarbaijan Shahid Madani University, Iran (In Persian).
44. Yang, A., Akhtar, S.S., Iqbal, S., Amjad, M., Naveed, M., Zahir, Z.A. and Jacobsen, S.E. (2016). Enhancing salt tolerance in quinoa by halotolerant bacterial inoculation. Functional Plant Biology, 43: 632-642. [DOI:10.1071/FP15265]
45. Ye, J., Zhang, W. and Guo, Y. (2013). Arabidopsis SOS3 plays an important role in salt tolerance by mediating calcium-dependent microfilament reorganization. Plant Cell Reports, 32: 139-148. [DOI:10.1007/s00299-012-1348-3]
46. Zhang, L., Li, S.F., Zhou, Q.H., Liu, Y.H., Zhang, J. and Qian, Z.Y. (2021). Subchronic toxicity study in rats evaluating herbicide-tolerant soybean DAS-68416-4. Regulatory Toxicology and Pharmacology, 119: 104833. [DOI:10.1016/j.yrtph.2020.104833]
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Karimi S, Pazhouhandeh M, Azizpour K. Evaluation of Some Characteristics of Substantial Equivalence of a Salinity-Resistant Transgenic Potato. pgr 2022; 9 (1) :43-56
URL: http://pgr.lu.ac.ir/article-1-257-en.html


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Volume 9, Issue 1 (2022) Back to browse issues page
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