Study of Expression Pattern of Some Transcription Factors in Wheat under Drought Stress and Zinc Nanoparticles

Raeesi Sadati, Seyede Yalda; Jahanbakhsh Godehkahriz, Sodabeh; Ebadi, Ali; Sedghi, Mohammad

doi:10.52547/pgr.7.2.11

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Volume 7, Issue 2 (2021)

pgr 2021, 7(2): 135-144

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Study of Expression Pattern of Some Transcription Factors in Wheat under Drought Stress and Zinc Nanoparticles

Seyede Yalda Raeesi Sadati

, Sodabeh Jahanbakhsh Godehkahriz ^*

, Ali Ebadi

, Mohammad Sedghi

Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran , jahanbakhsh@uma.ac.ir

Abstract: (10468 Views)

Under drought stress condition, the signaling system induces expression of certain genes to counteract the deleterious effects of environmental stress. Among the essential micronutrients for plant growth and development, zinc has an important role in many plant metabolic processes including gene expression and stress tolerance. In order to investigate the effect of drought stress and ZnO on relative expression pattern of some genes involved in abiotic stresses (including WRKY1, HMA2 and ZIP1 genes) in wheat cultivars, a factorial experimental was conducted in pot condition based on a completely randomized design with three replications. In this experiment, the first factor was three levels of drought stress (35, 60 and 85% of field capacity), the second factor was three wheat cultivars (including Heidari, Meihan and Sysons), and the third factor was three levels of ZnO (0, 0.5 and 1 g/l-1). According to the results, with increasing the level of drought stress, the relative expression of WRKY1 and ZIP1 genes in drought tolerant cultivar (Meihan), and also with increasing nanoparticle concentration over stress time, the expression of ZIP1 gene in drought sensitive cultivar (Sysons) increased. The highest relative expression of HMA2 gene was observed in Heidari cultivar under mild drought stress. Generally, the expression of all three genes studied in tolerant cultivar (Meihan) increased under drought stress. Increasing the expression level of HMA2 and ZIP1 genes could be related to the transfer of zinc to consuming tissues and also, to increase the consumption of zinc in current metabolism of plant, which is important in tolerance of wheat to drought stress.

Keywords: Gene expression, Drought stress, Transcription factors, Wheat, Zinc nanoparticle

Full-Text [PDF 537 kb] (1742 Downloads)

Type of Study: Research | Subject: Molecular genetics

References

1. Abbasi, A., Shekari, F., Mousavi, S.B. and Sabaghnia, N. (2016). Assessment of the effect of zinc sulfate biofortification on the quantity and quality characteristics of spring wheat cultivars. Advances in Bioresearch, 7(1): 18-25 ‌(In Persian).

2. Arab, K., Ravash, R. and Shiran, B. (2020). Evaluation of MYB93 and MAD8 genes in transgenic and non-transgenic rice. Plant Genetic Researches, 6(2): 33-42 (In Persian).

3. Bartels, D. and Sunkar, R. (2005). Drought and salt tolerance in plants. Critical Reviews in Plant Sciences, 24: 23-58. ‌ [DOI:10.1080/07352680590910410]

4. Cakmak, I. (2008). Enrichment of cereal grains with zinc: agronomic or genetic bio fortification? Plant and Soil, 302: 1-17.

5. Chen, W.R., Feng, Y. and Chao, Y.E. (2008). Genomic analysis and expression pattern of OsZIP1, OsZIP3, and OsZIP4 in two rice (Oryza sativa L.) genotypes with different zinc efficiency. Russian Journal of Plant Physiology, 55(3): 400-409.

6. Chen, L., Song, Y., Li, S., Zhang, L., Zou, C. and Yu, D. (2012). The role of WRKY transcription factors in plant abiotic stresses. Biochimica Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 18: 120-128. ‌ [DOI:10.1016/j.bbagrm.2011.09.002]

7. Davoodi, M.A., Towfighi, J. and Rashidi, A. (2013). Facile synthesis of carbon nanotube/nanofiber paper on a water-soluble support in one-step by chemical vapor deposition. Chemical Engineering Journal, 221: 159-165 ‌(In Persian).

8. Durmaz, E., Coruh, C., Dinler, G., Grusak, M.A., Peleg, Z., Saranga, Y. and Budak, H. (2011). Expression and cellular localization of ZIP1 transporter under zinc deficiency in wild emmer wheat. Plant Molecular Biology Reporter, 29: 582-596. ‌ [DOI:10.1007/s11105-010-0264-3]

9. Guo, P., Baum, M., Grando, S., Ceccarelli, S., Bai, G., Li, R., Von Korff, M., Varshney, R.K., Graner, A. and Valkoun, J. (2009). Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage. Journal of Experimental Botany, 60: 3531-3544.

10. Hosseinzadeh, S.R, Amiri, H. and Ismaili, A. (2016). Effect of vermicompost fertilizer on photosynthetic characteristics of chickpea (Cicer arietinum L.) under drought stress. Photosynthetica, 54: 87-92 ‌(In Persian).

11. Hwang, I., Jung, H.J. Park, J.I. Yang, T.J. and Nou, I.S. (2014). Transcriptome analysis of newly classified bZIP transcription factors of Brassica rapa in cold stress response. Genomics, 104: 194-202.

12. Hussain, D., Haydon, M.J., Wang, Y., Wong, E., Sherson, S.M., Young, J., Camakaris, J., Harper, J.F. and Cobbett, C.S. (2004). P-type ATPase heavy metal transporters with roles in essential zinc homeostasis in Arabidopsis. Plant Cell, 16(5): 1327-1339.

13. Javadi, S.M., Shabr, Z.S., Pour Abed, A. and Ghadiri, S. (2016). Reconstruction and analysis of drought tolerance gene networks in Hordeum vulgare leaf. The First International Conference and the 9th National Biotechnology Conference of the Islamic Republic of Iran. May 3rd to 5th, Tehran, Iran.

14. Khoshgoftarmanesh, A.H., Schulin, R., Chaney, R.L., Daneshbakhsh, B. and Afyuni, M. (2010). Micronutrient-efficient genotypes for crop yield and nutritional quality in sustainable agriculture. A review. Agronomy for Sustainable Development, 30: 83-107 ‌(In Persian).

15. Karim, M.R. and Rahman, M.A. (2015). Drought risk management for increased cereal production in Asian Least Developed Countries. Weather and Climate Extremes, 7: 24-35 ‌(In Persian).

16. Karami, S., Modarres-Sanavy, M., Ghanehpour, S. and Keshavarz, H. (2016). Effect of foliar zinc application on yield and, physiological traits and seed vigor of two soybean cultivars under water deficit. Nebulae Sciatica Biological, 8: 181-191 ‌(In Persian).

17. Mahmoudi Malhamlu, F. and Abdollahi Mandoulakani, B. (2019). Enhanced expression of superoxide dismutase, phenylalanine ammonialyase and bZIP33 transcription factor encoding genes under Zn deficiency conditions in bread wheat (Triticum aestivum L.). Cereal Research, 9(1): 17-26.

18. Niu, C.F., Wei, W.E.I., Zhou, Q.Y., Tian, A.G., Hao, Y.J., Zhang, W.K. and Chen, S.Y. (2012). Wheat WRKY genes TaWRKY2 and TaWRKY19 regulate abiotic stresses tolerance in transgenic Arabidopsis plants. Plant Cell and Environment, 35: 1156-1170.

19. Navabpour, S., Morris, K., Allen, R., Harrison, E., Mackerness, S. and Buchanan- Wollaston, V. (2003) Expression of senescence -enhanced genes in response to oxidative stress. Experimental Botany, 54: 2285-2292. ‌

20. Nimwegen, E. (2006). Scaling Laws in the Functional Content of Genomes. Springer, Boston, USA. [DOI:10.1007/0-387-33916-7_14]

21. Narimani, H., Seyed Sharifi, R., Khalilzadeh, R. and Aminzadeh, G. (2018). Effects of nano iron oxide on yield, chlorophyll fluorescence indices and some physiological traits of wheat (Triticum aestivum L.) under rain fed and supplementary irrigation conditions. Iranian Journal of Plant Biology, 10(3): 21-40.

22. Pask, A., Joshi, A.K., Manes, Y., Sharma, I., Chatrath, R., Singh, G.P., Sohu, V.S., Mavi, G.S., Sakuru, V.S.P., Kalappanavar, I.K., Mishra, V.K., Arun, B., Mujahid, M.Y., Hussain, M., Gautam, N.R., Barma, N.C.D., Hakim, A., Hoppitt, W., Trethowan, R. and Reynolds, M.P. (2014). A wheat phenotyping network to incorporate physiological traits for climate change in South Asia. Field Crops Research, 168: 156-167.

23. Pedas, P., Schjoerring, J.K. and Husted, S. (2009). Identification and characterization of zincstarvation- induced ZIP transporters from barley roots. Plant Physiology and Biochemistry, 47(5): 377-383.

24. Paygzar, Y., Ghanbari, A., Heidari, M. and Tavassoli, A. (2009). Effect foliar of micronutrients on the quantitative and qualitative characteristics of millet under drought stress (Pennisetumglacum) species notrifed. Iranian Journal of Agriculture Science, Islamic Azad University of Tabriz, 3: 67-78 ‌(In Persian).

25. Poormohammad Kiani, S., Maury, P., Nouri, L., Ykhlef, N., Grieu, P. and Sarrafi, A. (2009). QTL analysis of yield related traits in sunflower under different water treatments. Plant Breeding, 128: 363-373 ‌‌(In Persian).

26. Rahaie, M., Gomarian, M., Alizadeh, H., Malboobi, M.A. and Naghavi, M.R. (2012). The expression analysis of transcription factors under long term salt stress in tolerant and susceptible wheat genotypes using reverse northern blot technique. Iranian Journal of Crop Sciences, 13: 580-595 ‌‌(In Persian).

27. Rebey, I.B., Jabri-Karoui, I., Hamrouni-Sellami, I., Bourgou, S., Limam, F. and Marzouk, B. (2012). Effect of drought on the biochemical composition and antioxidant activities of cumin (Cuminum cyminum L.) seeds. Industrial Crops and Products, 36: 238-245. ‌

28. Rushton, P.J., Somssich, I.E., Ringler, P. and Shen, Q.J. (2010). WRKY transcription factors. Trends in Plant Science, 15: 247-258.

29. Ruijter, J.M., Ramakers, C., Hoogaars, W.M.H., Karlen, Y., Bakker, O., van den Hoff, M.J.B. and Moorman, A.F.M. (2009). Amplification efficiency: Linking baseline and bias in the analysis of quantitative PCR data. Nucleic Acids Research, 37(6): e45.

30. Sinclair, S.A., Senger, T., Talke, I.N., Cobbett, C.S., Haydon, M.J. and Kraemer, U. (2018). Systemic upregulation of MTP2-and HMA2-mediated Zn partitioning to the shoot supplements local Zn deficiency responses. Plant Cell, 30: 2463-2479.

31. Schmittgen, T.D. and Livak, K.J. (2008). Analyzing real-time PCR data by the comparative CT method. Nature Protocols, 3: 1101-1108.

32. Takahashi, R., Bashir, K., Ishimaru, Y., Nishizawa, N.K. and Nakanishi, H. (2012). The role of heavy-metal ATPases, HMAs, in zinc and cadmium transport in rice. Plant Signaling and‌ Behavior, 7(12): 1605-1607.

33. Taylor, K.M., Morgan, H.E., Johnson, A. and Nicholson, R.I. (2004). Structure-function analysis of HKE4, a member of the new LIV-1 subfamily of zinc transporters. Biochemical Journal, 377(1): 131-139.

34. Vishwakarma, M.K., Mishra, V.K., Gupta, P.K., Yadav, P.S., Kumar, H. and Joshi, A.K. (2014). Introgression of the high grain protein gene Gpc-B1 in an elite wheat variety of indo-gangetic plains through marker assisted backcross breeding. Current Plant Biology, 1: 60-67.

35. Yamaguchi-Shinozaki, K. and Shinozaki, K. (2005). Organization of cis-acting regulatory elements in osmotic-and cold-stress-responsive promoters. Trends in Plant Science, 10: 88-94.‌ [DOI:10.1016/j.tplants.2004.12.012]

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‎ 10.52547/pgr.7.2.11

‎ 20.1001.1.23831367.1399.7.2.8.7

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Raeesi Sadati S Y, Jahanbakhsh Godehkahriz S, Ebadi A, Sedghi M. Study of Expression Pattern of Some Transcription Factors in Wheat under Drought Stress and Zinc Nanoparticles. pgr 2021; 7 (2) :135-144
URL: http://pgr.lu.ac.ir/article-1-194-en.html

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