The aim was to study structure of the biochemical constituents of olive fruit show considerable changes during growth and ripening. In this investigation was studied some biochemical characterization (total sugar, photosynthetic pigments and total anthocyanin) on Koroneiki, T₂ and T₇ cultivars of olives in during fruit ripening in Ahvaz zone. So that, information on these parameters is important factor in quality assessment of olive products. Samples were harvested on 1th of July and on every 15th of each month till October. Photosynthetic pigments content was measured according to Lichtenthaler. Total anthocyanin was determined by the PH differential method. The soluble carbohydrates were determined using the phenol-sulphuric acid method. The highest and lowest content of total carotenoid belonged to Koroneiki cultivar (16.14 mg/g FW) at harvest 1 and to T₂ cultivar (2.64 mg/g FW) at harvest 6, respectively. The anthocyanin content of different olive cultivars increased in during fruit ripening. Highest of total anthocyanin content belonged to T₇ cultivar (0.033 mg/g FW) at harvest 6. The total sugar content decreased during ripening. The highest total sugar content of fruit belonged to Koroneiki cultivar (86.3 mg/g FW) at harvesting time 1 and the lowest to T₇ cultivar (7.7 mg/g FW)  at harvesting time 6 as well. In general, T₇ cultivar by high content of anthocyanin and adaptation to warm climatic conditions of ahvaz, showed its advantage in comparison to the other studied cultivars.