@article{ author = {Salami, Robab and Mohammadi, Seyed Abolghasem and Ghafarian, Sara and Moghaddam, Mohamm}, title = {Expression Analysis of Hv TIP2;3 and Hv TIP4;1 in Sensitive and Tolerant Barley Genotypes Under Salinity Stress}, abstract ={Barley is the most salt tolerant cereal‚ and is grown in a wide range of climatic conditions. To improve the plant tolerance to salinity‚ expression analysis of genes involved in stress tolerance could be effective in identification and development of tolerant genotypes. In this study, for evaluation of salinity effect on expression of Hv TIP2;3 and Hv TIP4;1 genes (encoding channel proteins in the membrane) in roots of barley, three genotypes; Clipper (salt susceptible), Sahara3771 (salt tolerant) and advanced breeding line (a salt tolerant line derived from a cross between Kavir and Sahra cultivars) were planted under 0, 100 and 200 mM NaCl. To determine the expression differences of these genes, RNA was extracted from root samples harvested at 24 hours, 3 days and 3 weeks after applying salinity treatments and cDNA was generated. Analysis of variance revealed a significant difference among genotypes, salinity levels and sampling stages for Hv TIP2;3 and Hv TIP4;1 genes expression pattern, whereas genotype × salinity interaction for Hv TIP2;3and genotype × sampling stage interaction were significant for both of the studied genes. The expression of TIP 2;3 gene in the 100 mM NaCl was increased in salt susceptible genotype Clipper and decreased in tolerant genotypes compared with control. Mean comparison of genotype and sampling combination showed that the expression level of Hv TIP4;1 gene at 3 weeks after salinity stress was increased in Sahara and advanced breeding line and decreased in Clipper. According to the results‚ it was confirmed that these genes are affected by salinity stress‚ and effective utilization of this genes in the barley in order to increase salinity tolerance could lead to desirable results. }, Keywords = {Aquaporins, Expression pattern, Tonoplast intrinsic proteins, Barley, Hv TIP2,3 and Hv TIP4,1 genes}, volume = {2}, Number = {2}, pages = {1-14}, publisher = {Lorestan University}, doi = {10.29252/pgr.2.2.1}, url = {http://pgr.lu.ac.ir/article-1-44-en.html}, eprint = {http://pgr.lu.ac.ir/article-1-44-en.pdf}, journal = {Plant Genetic Researches}, issn = {2383-1367}, eissn = {2676-7309}, year = {2016} } @article{ author = {SahranavardAzartamar, Fatemeh and Ghadimzadeh, Mortaza and Darvishzadeh, Rez}, title = {Genetic Diversity and Structure Analysis of Oily Sunflower (Helianthus annuus L.) Based on Microsatellit Markers}, abstract ={Knowledge about the amount of genetic diversity and understanding relationship between genotypes are important steps in plant germplasm conservation and breeding activities. In this study, the genetic diversity among 106 sunflower lines was assessed by 30 microsatellite primers. A total of 71 alleles were detected. Number of alleles in microssatellite loci ranged from 2 to 4 with the average number of 2.207 alleles per locus. The effective number of alleles ranged from 1.058 in locus ORS718 to 3.147 in locus HA3040. The average number of effective alleles was 1.641. The mean of PIC value was 0.344. Based on allele number and PIC value, SSR loci such as HA3040 and ORS733 are considered appropriate markers for studying genetic diversity in oily sunflower. Based on the results of cluster analysis using Jaccard's similarity coefficient and complete algorithm, the lines were grouped into four groups. Nineteen six out of 106 genotypes were grouped according to their origins (research centers). The highest and lowest Nei genetic distances were 0.21 and 0.004 between “NOVARTIS and HUNGARY” and “SPII with ENSAT and INRA-MONTPOL” groups, respectively. Analysis of the population structure revealed 5 subpopulations in the studied panel. The results show that the assignment of lines to subpopulations is not concordance with their geographical distribution pattern. The genetic diversity and distance revealed by SSR markers can be used in oily sunflower crossing and breeding programs}, Keywords = {Sunflower, Cluster analysis, Genetic diversity, Microsatellites, Subpopulation.}, volume = {2}, Number = {2}, pages = {15-32}, publisher = {Lorestan University}, doi = {10.29252/pgr.2.2.15}, url = {http://pgr.lu.ac.ir/article-1-45-en.html}, eprint = {http://pgr.lu.ac.ir/article-1-45-en.pdf}, journal = {Plant Genetic Researches}, issn = {2383-1367}, eissn = {2676-7309}, year = {2016} } @article{ author = {Shazdehahmadi, Marziyeh and Kharrazi, Mahi}, title = {Application of ISSR Molecular Markers for Genetic Diversity Study of Some Tobacco Genotypes}, abstract ={Determination of genetic diversity of breeding material is the first step in breeding programs. Evaluation of tobacco genetic diversity is essential for breeding programs and preservation of genetic resources. Genetic diversity level in tobacco genotypes, is very important for selection of parents in breeding programs. In this study, genetic diversity of 100 tobacco genotypes was evaluated using 25 ISSR markers. Banding pattern based on the presence or absence of the bands showed with 0 and 1, respectively. Out of 237 fragments produced in total cultivars, 195 bands were polymorphic and average of polymorphism ranged from 4 to 12 per primer. Average of polymorphism percentage was 94.10. To determine the efficiency of ISSR markers, PIC and their polymorphic percentage was calculated. UBC 818, UBC 812 and UBC 815 had the best marker parameters and were introduced as the best primers for assessment of genetic diversity. In order to evaluate the genetic similarity between cultivars, different similarity coefficient (SM, Dice and Jaccard) was calculated and Mantel corresponding test was performed. Finally, dendrogram was drawn based on SM similarity coefficient and UPGMA algorithm and the Cofenetic coefficient was calculated. All genotypes formed two distinct clusters indicating the high efficiency of used primers in amplification the approximate parts of the genome. The principle coordinate analysis showed that the first three components could explain 79.65 % of total variance. Totally, evaluation the tobacco genetic diversity using ISSR markers is suitable and ISSR marker can be used as appropriate marker system to identify the diversity and genetic relationship for breeding programs of this plant.}, Keywords = {Cluster analysis, Genetic diversity, Tobacco, Polymorphism, ISSR marker.}, volume = {2}, Number = {2}, pages = {33-46}, publisher = {Lorestan University}, doi = {10.29252/pgr.2.2.33}, url = {http://pgr.lu.ac.ir/article-1-46-en.html}, eprint = {http://pgr.lu.ac.ir/article-1-46-en.pdf}, journal = {Plant Genetic Researches}, issn = {2383-1367}, eissn = {2676-7309}, year = {2016} } @article{ author = {Darvishian, Ali and Ismaili, Ahmad and Nazarian-Firouzabadi, Farhad and MirDrikvand, Reza and Hosseinpour, Tahmasb}, title = {Assessment of Genetic Diversity among Wheat Genotypes of West Iran, Using Randomized Markers}, abstract ={Plant breeding is selection of advanced genotypes and its progress depends on correct evaluation of genetic variation. Among different selection procedure, molecular markers have a good potential for evaluation of variation. In this research, RAPD molecular markers were used to evaluation of genetic diversity among 25 wheat cultivars and advanced breeding lines. Genomic DNA was extracted from leaves by Dellaporta method and 30 primers were used for PCR amplification. Results of Primers led to 200 storable electrophoretic bands which 130 of them (65%) were polymorphic. F4 and A18 primers produced the greatest and lowest band, respectively. Cluster analysis was performed based on band presence (1) and absence (0) using Jaccard coefficient similarity and UPGMA method. Similarity coefficient ranged from 0.22 to 0.87 with an average of 0.64. The highest similarity (0.87) was observed between Azar2 and Sardari and lowest similarity (0.22) was observed between Seimareh and BAVICORA. With cut of line on 0.72 in dendrogram, 6 main groups were clustered and other genotypes were clustered in different group. Regarding to the high similarity among these genotypes, it is necessary to develop the wheat germplasm in related research centers.}, Keywords = {Genetic similarity, Genetic diversity, Wheat, RAPD}, volume = {2}, Number = {2}, pages = {47-56}, publisher = {Lorestan University}, doi = {10.29252/pgr.2.2.47}, url = {http://pgr.lu.ac.ir/article-1-47-en.html}, eprint = {http://pgr.lu.ac.ir/article-1-47-en.pdf}, journal = {Plant Genetic Researches}, issn = {2383-1367}, eissn = {2676-7309}, year = {2016} } @article{ author = {Mohammadi, Mohtasham and Roustaie, Mozafar}, title = {Estimation of Genetic Parameters of Grain Yield and Some Agronomic Traits in Bread Wheat Using Diallel Crosses}, abstract ={For development of high yield wheat cultivars, this study was conducted to estimate the general combining ability (GCA) of parents, specific combining ability (SCA) of hybrid progenies, heritability and heterosis of grain yield and some agronomic traits. A partial diallel crosses which obtained in a set of six wheat genotypes were sown in randomized complete block design at Gachsarann agricultural research station in 2013-14 growing season under dryland condition. The results of this study revealed a significant difference among the genotypes for all of the traits that indicating considerable genetic variation. Significant effects of GCA and SCA indicated role of additive and non-additive gene action in the control of all considered traits. The mean square ratio of GCA to SCA and Baker genetic ratio showed a preponderance of non-additive gene action for all of the studied traits. Broad-sense heritability was high (0.67-0.99) for all of them, which indicated the role of genetic factors compared with non-genetic factors for controlling of these traits. Narrow-sense heritability was low to moderate (0.06–0.37). Among the parents, Koohdasht was the best general combiners for early growth vigor, days to maturity, spike length, peduncle length, flag leaf extrusion and grain yield. The Koohdasht × DAMARA-6 hybrid showed the best specific cross for days to heading, days to maturity, grain filling period, plant height, chlorophyll content, canopy temperature, grain length and grain yield. These hybrids showed positive and significant heterosis for grain yield, chlorophyll content and grain length and negative heterosis for plant height. It is expected to produce desirable segregants and could be exploited successfully in wheat improvement programs. In addition, because of preponderance of non-additive gene action for studied traits, particularly in the early generations, efficiency of genetic selection was low and selection for genetic improvement of these traits must be retraced in advanced generations.}, Keywords = {Diallel cross, Specific combining ability (SCA), General combining ability (GCA), Wheat, Heterosis}, volume = {2}, Number = {2}, pages = {57-72}, publisher = {Lorestan University}, doi = {10.29252/pgr.2.2.57}, url = {http://pgr.lu.ac.ir/article-1-61-en.html}, eprint = {http://pgr.lu.ac.ir/article-1-61-en.pdf}, journal = {Plant Genetic Researches}, issn = {2383-1367}, eissn = {2676-7309}, year = {2016} } @article{ author = {Taheri, Ensieh and Shirzadian-Khorramabad, Reza and Sharifi-Sirchi, Gholamreza and Sabouri, Atefeh and Abbaszadeh, Khadijeh}, title = {Assessment of Genetic Diversity of Three Yarrow\'s Wild Masses in Hormozgan Province Using Morphological Traits}, abstract ={Yarrow plant or plain short type yarrow, known as Achillea wilhelmsii C. kock, belongs to Asteraceae family. The present study was conducted in order to evaluate three different yarrow populations for determination of their genetic relationship, using morphological traits in the form of nested design as completely randomized with ten replications. General heritability of all traits ranged from 98 to 100, indicating a high heritability for these traits. The highest coefficient of genetic variation belonged to root diameter (1.66), indicating the existence of hifh diversity among genotypes. The lowest coefficient of genetic variation belonged to leaf length to width ratio (0.36), suggesting a low level of variation for this character. Based on PCA analysis, the first two components justified almost 90 percent of total variations. The three populations were nearly separated according to biplot analysis. Also, cluster analysis confirmed the biplot results and the populations were identified as three separated clusters which reflect the apparent difference among yarrow populations. The results of this study showed a wide genetic diversity for evaluated populations with regarding measured traits in Hormozgan province. Aforementioned findings indicated a dormant potentials of native yarrow populaton in south Iran and high value of these resources and obviously more attention need to be paid to identify, maintenance, assessing and apply them in breeding programs.}, Keywords = {Cluster analysis, Correlation coefficient, Morphologic markers, Achillea}, volume = {2}, Number = {2}, pages = {73-82}, publisher = {Lorestan University}, doi = {10.29252/pgr.2.2.73}, url = {http://pgr.lu.ac.ir/article-1-49-en.html}, eprint = {http://pgr.lu.ac.ir/article-1-49-en.pdf}, journal = {Plant Genetic Researches}, issn = {2383-1367}, eissn = {2676-7309}, year = {2016} }