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Showing 2 results for Transgenic
Mahdieh Sahshorpour, Jafar Amani, Mahyat Jafari, Ali Hatef Salmanian,
Volume 1, Issue 1 (5-2014)
Abstract
One of the important pathogens which cause hemorrhagic colitis and hemolytic uremic syndrome in humans is enterohemorrhagic Escherichia coli (EHEC) O157:H7. Cattle are the most important reservoir of this bacterium. EspA and Intimin are two protein factors for bacteria colonization on intestinal epithelium and cause attaching/effacing lesion. The LEE pathogenicity islands code these proteins. EspA is part of type III secretion systems which delivers Tir to the host cell and integrate to membrane. Intimin encoded by eae gene and fused to Tir. In this research we supposed that chimeric recombinant form of EI gene containing EspA and Intimin were fused with a linker as an edible candidate vaccine would reduce colonization of E. coli O157:H7 in animal model. We constructed a synthetic gene EspA (E120) and intimin (Int282) fused by (EAAAK)4 sequence. The synthetic gene (EI) was codon optimized and subcloned into plant expression vector (PBI121) under CaMV35S promoter and then transferred to tobacco plant by agrobacterium mediated protocol. The presence of inserted gene in plant genome was documented by PCR and RT-PCR methods. The amount of EI protein in transgenic tobacco leaves were estimated 0.1% of the total soluble protein (TSP) by ELISA method.
Samira Karimi, Maghsoud Pazhouhandeh, Kambiz Azizpour,
Volume 9, Issue 1 (9-2022)
Abstract
Transgenic plants and their products are being developed day by day due to their improved characteristics, and it is necessary to evaluate the safety of these plants before releasing them. Hence, the importance of the issue of biosafety of transgenic plants and the use of their products has led the regulatory agencies to create some laws called substantial equivalence. based on that, the essential nutrients of transgenic plants are examined and compared with the control. The present study aimed to compare the transgenic potato line F (salinity-resistant) with non-transgenic Agria cultivar plants. The salt resistant potato line was produced by transferring Arabidopsis SOS3 gene to potato (Agria variety) and its resistance was confirmed. First, the presence of AtSOS3 gene in F-line plants was confirmed and then the substantial equivalent experiments were performed by comparing the production of proline, soluble sugars, carotenoids and chlorophylls a and b, the relative expression of Catalase1 (CAT1) and AtSOS3 gene between F and non-transgenic WT Agria plants. Based on evaluations of physiological traits and some metabolites (proline content, soluble sugars, carotenoids and chlorophylls a and b) and morphological traits (plant height, dry and fresh weight of plant) between line F and WT, no significant difference was observed. The number of microbiome colonies around the root in the transgenic F and non-transgenic WT plants was a non-significant difference, which indicates that the transgenic line has no threatening effects on the environment and human pathogenicity. The relative expression of AtSOS3 and Catalase1 genes in line F had higher values than WT. The reason for such increase in the expression of Catalase1 is the activation of plant defense mechanisms against stress. Finally, the results of the evaluations proved the equality of line F and WT
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