|
|
|
 |
Search published articles |
 |
|
Showing 2 results for Specific Marker
Esmaeil Dasturani, Khalil Zaynali Nezhad, Masood Soltani Najafabadi, Mohammadhadi Pahlevani, Hassan Soltanlo, Saeed Bagherikia,
Volume 8, Issue 1 (8-2021)
Abstract
The aim of this study was to determine the haplotype groups and identify the specific alleles associated with desirable agronomic characteristics in bread wheat. For this purpose, 42 local bread wheat genotypes belong to Iran region and nine commercial cultivars along with Chinese Spring variety (reference genotype) were cultivated in the format of augmented design and evaluated based on their 13 phenotypic traits. The results of descriptive statistics showed that awn length and day to flowering had the highest and lowest phenotypic coefficient of variation, respectively. Eight microsatellite markers were used to investigate the haplotype variation of QTLs associated with phenotypic traits located on wheat chromosomes 4B and 7D. The result showed that the genotypes were classified into 13 and 6 haplotype groups according to the allelic comparison with the reference genotype on chromosome 4B and 7D, respectively. In order to investigate the relationship between traits and markers, analysis of variance was performed based on completely randomized design with unequal numbers of replications for each marker. In general, of the 13 traits studied, there was a statistically significant linkage for eight traits and for the three traits, an allele-specific was introduced simultaneously. If the breeders are interested in genotype selection that simultaneously have three desirable characteristics such as early anthesis, semi-dwarfing and a greater number of grains per spike, they can use an allele-specific (153 bp) of Xgwm149-4B marker.
Fatemeh Bagherzadeh, Hannaneh Mirahmadi, Soraya Pourtabrizi, Ali Kazemipour, Maryam Dorraninejad, Roohollah Abdoshahi,
Volume 11, Issue 1 (9-2024)
Abstract
Stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases of wheat in many parts of the world. Genetic resistance is the best way to deal with this disease. This disease evolves very quickly, and for this reason, the resistance of many major-effect resistance genes has been overcome. In this study, the Yr5 and Yr15 genes, which are among the most effective resistance genes in a full-grown plant stage, were introduced to the Iranian cultivars, including Baharan, Rakhshan, Setareh, Sivand, Parsi, and Amin using the marker-assisted backcrossing method. These genes were intrroduced to the mentioned cultivars in six distinct breeding projects. Cultivars were crosses with the donor parents Yr5/6*Avocet‘S’and Yr15/6*Avocet‘S’. The progeny of F1 generation, were backcrossed with the Iranian cultivars (recurrent parent) to obtain BC1F1 progeny in each population. By genotyping 30 random plants in each project, the heterozygous genotypes carrying the resistance genes were identified using specific markers, and the second backcrossing was performed. Overall, the results of this studuy demonstrated that within each population, a stripe rust-resistant line can be developed through repeated generations of backcrossing followed by one generation of selfing. The introduction and pyramiding of yellow rust resistance genes into the Iranian cultivars not only enhance durable resistance and mitigate yield losses during epidemic years but also can contribute to benefits to the environment.
|
|
