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Showing 2 results for Salt Stress
Razieh Ghorbani, Raheleh Ghasemzadeh, Hadi Alipour,
Volume 9, Issue 1 (9-2022)
Abstract
In order to identify loci controlling seedling morpho-physiologic characteristics in 88 bread wheat cultivars, a greenhouse experiment based on simple alpha lattice was conducted under both normal and 120 mM (12 ds/m) salt stress condition of the Faculty of Agriculture, Urmia University in 2020-2021 cropping season. Chlorophyll a, b and carotenoid content, proline, plant fresh and dry weight, plant height and leaf relative water content (RWC), Na+, K+ and K+/Na+ concentrations were measured. After genotyping by sequencing with Ion Torrent technology and removal of SNPs with more than 20% of missing data and minor allele frequency less than 5%, a total of 5869 SNP markers were identified. Based on association mapping with the mixed linear model (MLM) method, a total of 25 marker-trait associations were detected under normal conditions. The A and D genomes had the highest and lowest number of significant marker-trait associations (MTAs). Among the studied traits under normal conditions, chlorophyll a had the highest number of MTAs on 1A, 3B, 3D, 5B, 7A chromosomes with eight MTAs. A total of 21 MTAs were identified under salt stress conditions which the genome B and D had the highest and lowest number of MTAs, respectively. Five MTAs were identified for plant fresh weight, which were located on chromosomes 4A and 6B. The results of this study provide valuable information about the loci associated with the studied traits, which can be used in marker assisted selection in wheat breeding programs after confirmation in biparental populations and additional experiments.
Khadijeh Abbaszadeh, Reza Shirzadian-Khorramabad, Mohammad Mahdi Sohani, Zahra Hajiahmadi,
Volume 9, Issue 2 (3-2023)
Abstract
Salinity stress affects morpho-physiological and biochemical traits of plants. The transgenic Bt plants play a significant role in pest control, but their response and ability to cope with environmental stresses still need to be evaluated. Therefore, effect of salinity stress at 0, 50, 100, 150, and 200 mM on morphological, physiological, and molecular traits of T3 transgenic tomato plants containing cry1Ab gene (CH-Falat-Bt) was investigated and compared with that of the non-transgenic control (CH-Falat). Evaluation of the morphological traits (leaf area, root length, fresh and dry weight of roots) at different salinity levels revealed that CH-Falat-Bt transgenic plants are more tolerant to salinity stress compared to CH-Falat non-transgenic plants. The chlorophyll content at 150 and 200 mM salinity levels was 12 and 9% plants, respectively. Moreover, the amount of RWC, carotenoids, proline and soluble sugars increased significantly in transgenic plants as salinity levels increased. The relative expression of SOS1 and SOS2 genes showed a significant increase in all salinity levels in CH-Falat-Bt transgenic plants compared to CH-Falat non-transgenic plants. The amount of electrolyte leakage in the transgenic plants was significantly reduced compared to the non-transgenic plants. The results of morphological, physiological, and molecular investigations of CH-Falat-Bt transgenic plants confirmed that the undesirable effects of salinity stress on transgenic plants is much less than non-transgenic ones. in general CH-Falat-Bt transgenic plants are more tolerant to different applied salinity levels than the wild variety.
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