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Showing 3 results for Linkage Map
Atefeh Kaviani Charati, Hossein Sabouri, Hossein Ali Fallahi, Eisa Jorjani,
Volume 3, Issue 1 (9-2016)
Abstract
Abstract In order to genetic analysis of spike characteristics in barley, an experiment was conducted with 100 F3 and F4 barley families derived from Badia × Komino cross at Research Farm college of Agricultural University of Gonbad Kavous (Iran) based on randomized complete block design with three replications. Agronomic traits such as spike length, number of seeds per spike, total of spike, total weight of spike, grain length and grain diameter were measured. Linkage map with 7 SSR and 69 polymorphic alleles of iPBS markers were prepared which covered 632.2 cM of barley genome. QTL analysis was performed based on the method of composite interval mapping (CIM). Ten QTLs (with additive effect ranged from 127.07 for spike number to -0.625 mm for grain length) were detected. Phenotypic variance explained by QTLs ranged from 10.9 to 12.9 percent, which the highest related to spike length in F3 generation and the lowest related to the total number of spikes in F3 generation and the total weight of spike in F4 generation. All detected QTL were major effects and after validation can be used in breeding programs and marker-assisted selection.
Mohammad Reza Jafarzadeh Razmi, Saeid Navabpour, Hossein Sabouri, Seyedeh Sanaz Ramezanpour,
Volume 6, Issue 2 (3-2020)
Abstract
In order to analyze the genetic components of agronomic traits among 116 F9 recombinant lines derived from crosses of Ahlamitarom × Sepidroud rice cultivars, an experiment was conducted as a randomized complete block design in research farm of Gonbad Kavous University of Agriculture with three replications in 2016 and 2017. Genetic linkage map provided with 80 SSR markers, 28 iPBS Markers (79 polymorphic alleles), 7 IRAP markers (17 polymorphic alleles) and 26 ISSR markers (70 polymorphic alleles), which covered 1275.4 cM of the rice genome. QTL analysis was performed by Composite Interval Mapping. In two years, 15 QTLs detected for the studied traits. The additive effected varied from 6.725 g for grain weight up to -85.626 g for grain weight. Also, R2 for the detected QTLs explained from 11.3% to 20% of the total variation. The highest R2 was related to grain weight in the first year of experiment. Among the detected QTLs, qGWs on chromosome 1, were found to be stable and large effector QTLs for rice (Oryza sativa L.) grain weight, and can be used in marker-assisted breeding and selection programs after validation.
Mahnaz Katouzi, Saeid Navabpour, Hossein Sabouri , Ali Akbar Ebadi,
Volume 7, Issue 2 (3-2021)
Abstract
In order to identify QTLs controlling agronomically traits, landrace Tarom and rice Tarom mutant were crossed. SSR, ISSR, iPBS and IRAP markers were amplified in 250 F2 individuals to prepare the linkage map. Number of tillers, 100 grain weight, number of filled grains, number of unfilled grains, plant height, panicle length, number of branches, stem diameter, grain length, grain width, grain shape, straw weight, days to maturity, flag leaf length and flag leaf width were measured for 250 individuals. The linkage map covered 970.9 cM of rice genome. The distance between two adjacent markers was calculated to be 12.77 cM. Based on the results, a total of 13 QTLs were identified for the evaluated traits. For all studied traits, alleles transferred from the parents to the QTLs detected increased grain yield. Most QTLs were detected for days to flowering. Three QTLs were located on chromosomes 10 and 4 (two QTLs) for days to flowering. qLDF-4a and qLDF-4b had a negative additive effect and the parent alleles of the mutant landrace Tarom reduced the number of days to flowering. These QTLs explained 11.6% of the phenotypic variance. Since the population under study was derived from a cross between landrace and mutant Tarom cultivars and the resulting population varied only in the mutated genes; so, the QTLs detected in this study were more accurate in location and expression levels, and after validation of them, they could be recommended for marker assistant selection breeding programs.
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