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Phosphorus is one of the important macronutrients involved in various physiological and metabolic pathways. It has also major role in development and transmission of energy. To map QTLs for the traits associated with phosphorus accumulation at shoot stage in barley, 148 doubled haploid lines derived from a cross between Sahara3771 and Clipper cultivars were evaluated in greenhouse condition. Analysis of variance showed, that significant differences among the lines for all traits. Transgressive segregation was observed for all traits. Linkage map of population consist of 246 SSR, EST-SSR markers, 238 RFLP, 26 retrotransposone markers including IRAP, REMAP and a morphological marker that coverd 1099.09 cM of barley genome and an average distance of 2.15 cM between two adjacent markers. In total, 13 QTLs were identified for phosphorus concentration and content at five-leaf and maturity stages. For phosphorus concentration and content at five leaf stages three and two QTLs were found, respectively. Four QTLs were detected for phosphorus concentration at maturity stage. Out of which three and one QTL showed positive and negative additive effects, respectively. For phosphorus content of single plant at maturity stage, four QTLs explaining 60% of phenotypic variance were mapped. Out of 13 QTLs identified for the trait, 10 QTLs had positive additive effects, indicating the role of Clipper alleles in this loci in increasing the related traits value in offspring. In the present study, one common QTL identified which could be due to genetic linkage or pleiotropic effect.