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Showing 2 results for Genetic Distance
Saeid Navabpour, Ahad Yamchi, Sasan Golcheshmeh,
Volume 8, Issue 1 (8-2021)
Abstract
The present study was performed to classify and study genetic diversity between Calotropis procera accessions from different regions of Kerman province (Iran) using ISSR markers. In total, DNA from 14 plant samples with nine ISSR primers was amplified by PCR and their banding pattern was obtained. The primers showed acceptable polymorphism (35.93) and minimum and maximum polymorphic information content (PIC) of primers in this study were 0.11 for ISSR9 primer and 0.41 for ISSR3 and ISSR8 primers, respectively. Genetic similarity based on Nei index was varied from 0.405 to 0.745 and the lowest genetic similarity was found between J3 (Related to Jiroft) and D2 (Related to Dosari) and the highest genetic similarity was found between J1 and J2 (both of them for Jiroft). By using UPGMA cluster analysis, samples divided into four groups, and the second and third groups contained more accessions. In terms of genetic similarity, two accessions of Jiroft 1 (J1) and Jiroft 2 (J2) which classified in the same cluster were closer. Also, the accessions collected from Anbarabad were at a longer genetic distance than other accessions. Principal coordinate analysis also showed that the first and second components justify 67 percent of obtained genetic diversity. In general, ISSR markers were useful for classifying Calotropis procera accessions and according to the obtained information about existence of genetic diversity between Calotropis procera accessions of Kerman province, this diversity could be useful in the future for breeding and production of Calotropis procera.
Faraneh Roshan, Mohammad Rabiei, Behrouz Shiran,
Volume 11, Issue 1 (9-2024)
Abstract
Violet plants (Viola sp.) belonging to the Violaceae family are ornamental plants that could be used for drug design due to their cyclotidic compounds. In this study, 21 different ecotypes of violets were collected from the northern regions of Iran. After DNA extraction, the genetic diversity of ecotypes was investigated using the iPBS molecular marker. Twelve iPBS primers used during the present investigation resulted in 214 bands. The average percentage of observed polymorphism, polymorphic information content (PIC), and marker index (MI) were calculated to be 31.92%, 0.35%, and 5.64% respectively. The Nei genetic distance index ranged between 0 and 0.66. The results indicate a considerable genetic diversity among the violet ecotypes and the efficiency of the iPBS marker in detecting polymorphism. The population genetic analysis showed that 61% of the diversity is related to intra-species diversity. The species V. odorata and V. alba exhibited the greatest degrees of polymorphism, effective allele number, Shannon index value, and heterozygosity ratios. Also, the dendrogram depicted the close genetic relationship between these two species, as evidenced by Nei's genetic distance measurements. In general, considering the existing taxonomic information and the results obtained from this experiment, it can be concluded that the use of the iPBS marker is highly effective in systematic studies of the genus Viola. The results of this experiment led to the appropriate differentiation of ecotypes and species, which could be used in further breeding studies.
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