After cereals, oilseeds are the second-largest food reserves in the world. According to available statistics, more than 95 percent of Iran's oil needs are imported. Given the growing need for edible oils in Iran, it is important to identify fatty acids in the oilseed crops. Camelina sativa L. is an oil-medicinal plant and belongs to the Brassicaceae family that requires very little water and fertilizers. It is known as a low input plant. In this study, to analyze the fatty acid profile for breeding programs and specific industries, 137 doubled haploid camelina lines were evaluated in terms of fatty acid composition and variability of fatty acids trait, to estimate phenotypic coefficient of variation (PCV), genotypic coefficient of variation (GCV), heritability, and expected genetic advance. The determination of fatty acid by gas chromatography showed that 18 types of fatty acids were detectable in camelina seed oil. It is shown that the two fatty acids (C14:0 and C16:1) have the highest PCV and GCV. The highest heritability for C20:2, C20:3 and C20:0 fatty acids was estimated 98.92, 98.59 and 96.49 percent, respectively. In this study, two lines with linoleic acid of 35.81-36.67% and four lines with values ranged from 22.08-23.00% were introduced. The ratio of omega-6 to omega-3 (0.479-0.759) was obtained in the studied lines.