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Showing 3 results for Transcription Factors
Kobra Arab, Rudabeh Ravash, Behrouz Shiran,
Volume 6, Issue 2 (3-2020)
Abstract
Increasing drought tolerance, especially in rice, which is one of the most important crops in Asia, is necessary. Transcription factors are specific sequence DNA-binding proteins that are capable of activating or suppressing transcription. These proteins regulate gene expression levels by binding to cis regulatory elements in the promoter of target genes to control various biological processes such as growth, cell division and response to environmental stresses. In this study, MAD8 and MYB93 genes that were involved in drought stress in rice were considered in two leaf and anther tissues at 0, 24, 48, 72 h and one week after stopping irrigation. The results of q-PCR analysis showed significant expression changes of these transcription factor genes under drought stress conditions. In this study, a significant increase in the expression of these genes at 24 h after drought stress in transgenic plants (Promoter region with accession: NC_029264.1 and GUS gene have transformed) compared to non-transgenic plants showed a relationship between these transcription factors and higher expression of transported promoter in transgenic plants.
Seyede Yalda Raeesi Sadati, Sodabeh Jahanbakhsh Godehkahriz, Ali Ebadi, Mohammad Sedghi,
Volume 7, Issue 2 (3-2021)
Abstract
Under drought stress condition, the signaling system induces expression of certain genes to counteract the deleterious effects of environmental stress. Among the essential micronutrients for plant growth and development, zinc has an important role in many plant metabolic processes including gene expression and stress tolerance. In order to investigate the effect of drought stress and ZnO on relative expression pattern of some genes involved in abiotic stresses (including WRKY1, HMA2 and ZIP1 genes) in wheat cultivars, a factorial experimental was conducted in pot condition based on a completely randomized design with three replications. In this experiment, the first factor was three levels of drought stress (35, 60 and 85% of field capacity), the second factor was three wheat cultivars (including Heidari, Meihan and Sysons), and the third factor was three levels of ZnO (0, 0.5 and 1 g/l-1). According to the results, with increasing the level of drought stress, the relative expression of WRKY1 and ZIP1 genes in drought tolerant cultivar (Meihan), and also with increasing nanoparticle concentration over stress time, the expression of ZIP1 gene in drought sensitive cultivar (Sysons) increased. The highest relative expression of HMA2 gene was observed in Heidari cultivar under mild drought stress. Generally, the expression of all three genes studied in tolerant cultivar (Meihan) increased under drought stress. Increasing the expression level of HMA2 and ZIP1 genes could be related to the transfer of zinc to consuming tissues and also, to increase the consumption of zinc in current metabolism of plant, which is important in tolerance of wheat to drought stress.
Seyede Maryam Seyed Seyed Hassan Pour, Leila Nejadsadeghi, Zahra Sadat Shobbar, Danial Kahrizi,
Volume 10, Issue 2 (2-2024)
Abstract
Camelina )Camelina sativa (is an annual, self-pollinating, allohexaploid plant with diploid inheritance belonging to the Brassicaceae family. Camelina exhibits a remarkable degree of similarity to the model plant Arabidopsis thaliana. WRKY transcription factors are among important gene families in plants that play crucial roles in regulating growth and development and in response to diverse stresses. In this research, using bioinformatics analysis and databases, members of the WRKY gene family were identified and their various characteristics were investigated. Overall, the genome of the Camelina plant was found to harbor 214 members of the WRKY gene family. All 214 WRKY genes were found to possess the conserved WRKY functional domain, along with a variety of motifs within their structural composition. Phylogenetic analysis divided the identified members of Camelina WRKY genes into four main groups. Examination of the chromosomal positions revealed that the 214 identified WRKY genes exhibited an uneven distribution across the chromosomes. In order to validate the identified genes, the expression of two genes (Csa11g065620 and Csa07g035970) orthologs of two genes involved in drought stress in Arabidopsis (WRKY8 and WRKY57), were investigated in a drought tolerant (DH 91) and a drought sensitive (DH 101) lines. The results of the gene expression analysis showed that both genes had high expression in drought stress conditions in tolerant line in comparison to normal conditions, whereas no significant expression was found in drought sensitive line. The findings of the present study offer valuable insights for evolutionary investigations and enhance our understanding of the functional roles of the WRKY gene family in Camelina, thereby laying a foundation for future research endeavors in this field.
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