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Showing 7 results for Gene Expression
Mohammad Majdi, Ghasem Karimzade, Mohammad Ali Malboobi,
Volume 1, Issue 2 (2-2015)
Abstract
Feverfew (Tanacetum partheniumL. Schulz Bip.) is a medicinal herb belonging to the Asteraceae which recently raised researcher’s attention due to its medicinal value and pharmacological activities, especially as a migraine prophylaxis agent and also for treatment of cancer. Parthenolide has a sesquiterpene lactone structure which is most likely synthesized through the mevalonic acid (MVA) pathway. Recently, it has been shown that there is cross a talk between the MVA and the MEP pathways through IDP (Iosopentenyl diphosphate) exchange as a precursor for the biosynthesis of different terpenes hence, parthenolide biosynthesis could be affected by the MEP pathway as well. In the present work, the relative gene expression of two key genes of the MVA pathway, including 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) and germacrene A synthase (GAS) and two key genes of the MEP pathway including 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) and hydroxy-2-methyl-2-(E)-butenyl4-diphosphate reductase (HDR) were examined in leaves derived from the vegetative or generative phases, also in flowers using real time PCR. The results of our study showed that expression of these genes depend on the growth stage and genotype. Moreover, in which in the flowers and leaves derived from vegetative phase in different genotypes only the TpGAS gene expression showed a significant difference, while in the leaves derived of generative phase the relative gene expression showed a significant difference for TpHMGR, TpGAS and TpDXR.
Saeed Bagherikia, Mohammadhadi Pahlevani, Ahad Yamchi, Khalil Zenalinezhad, Ali Mostafaie,
Volume 4, Issue 1 (9-2017)
Abstract
Under drought stress conditions, as one of the most important limiting factors of grain yield in wheat at arid and semi-arid regions, the remobilization of assimilates gain would be more valuable to grain filling. There are a few reports on the importance of remobilization of the root during the grain filling period under drought stress conditions. An advanced mutant line of bread wheat (T-65-7-1) along with its wild type (cv. Tabasi), were planted at two moisture conditions (normal and 30-40% of field capacity) as a factorial experiment based on a completely randomized design with three replications. Sampling for gene expression analysis was conducted from the root in two stages (7 and 21 days after anthesis). In these genotypes, fructan remobilization, efficiency of fructan remobilization, and relative expression of genes involved in the synthesis and hydrolysis of fructan during the grain filling period, in root, were studied under terminal drought stress. The results showed that the stored fructan in the root participated in the assimilate remobilization. Higher fructan remobilization through root to grain in mutant line under drought stress conditions was due to over-expression of genes involved in the synthesis of fructan (1-SST and 6-SFT) at 7-days after anthesis and in hydrolysis of fructan (6-FEH) at 21-days after anthesis, compared to wild type. Drought stress did not cause a significant change in gene expression of 1-FFT and 1-FEH genes in the root of both genotypes, which confirms the only β (2,6) linkages as predominant form of fructan has affected under drought stress conditions. In wheat breeding programs, 1-SST, 6-SFT and 6-FEH can be used as molecular markers for selecting genotypes with high fructan content and more remobilization.
Mehrnoosh Rafeie, Mohammad Reza Amerian, Behzad Sorkhi, Parviz Heidari, Hamid Reza Asghari,
Volume 6, Issue 2 (3-2020)
Abstract
To investigate the effect of exogenous brassinosteroid application on grain yield, catalase, chlorophyll content, membrane mtability index and gene expression of some genes involving in brassinosteroid signaling pathway (BES1 and BRI1) under drought stress, a split-split plot on randomized complete block design with three replications was conducted at the experimental field of Seed and Plant Improvement Institute, Karaj, Iran in 2019. The main factor was two irrigation treatments (normal irrigation and water holding after 50% flowering stage), the subplots were four concentrations of brassinosteroid (0, 0.25, 0.625 and 1 mg/l) and seven genotypes (Mehregan, Paris, 2858, 3505, 3737, 4228 and 4056) were considered as sub-sub plots. Samples were taken at 30 days after 50% flowering stage (zadoks 89) from flag leaves. The results showed that drought stress significantly reduced grain yield, chlorophyll content, membrane stability index and increased catalase in all genotypes. Genotype 4228 was identified as the most tolerant genotype among unknown wheat genotypes based on grian yield, chlorophyll content, membrane stability index and catalase. Also, the result revealed that applied epibrassinolide could reduce the destructive effects of drought stress on wheat thus grain yield was enhanced under drought stress in all genotypes by increasing the aforementioned traits. Forethermore, grain yield was increased by rising the epibrasinolide concentration. Gene expression pattern of TaBES1 and TaBRI1 using real-time PCR showed that although brassinosteroid enhances drought tolerance in wheat but its signaling pathway is different from the BRI1 signaling pathway.
Seyed Sajad Sohrabi, Seyyed Mohsen Sohrabi, Seyed Karim Mousavi, Mohsen Mohammadi,
Volume 7, Issue 1 (9-2020)
Abstract
Saffron (Crocus sativus L.) is the most valuable and expensive spice in the world. The stigmas of saffron are the source of valuable apocarotenoids such as crocin, picrocrocin and safranal. transcriptomic and expression studies of genes are important steps in investigating of secondary metabolites in plants. One of the important prerequisites for such studies is the existence of reliable and stable reference genes to normalize the expression of other genes. In the present study, eight reference genes were identified and isolated using transcriptome of saffron and their expression stability was evaluated by nonparametric statistics and methods. The results of amplification and sequencing showed accurate identification of eight reference genes Actin, EF1, GAPDH, H3, MDH, TBP, UBC and UBQ. The expression stability evaluation revealed that MDH and UBQ genes had the highest stability among different saffron tissues and TBP had the lowest stability among them. In this study, for first time, eight reference genes were isolated from saffron and their expression stability was evaluated. The reference genes identified in the present study can be used as stable genes to normalize gene expression in transcriptomic and expression studies of saffron plant.
Seyede Yalda Raeesi Sadati, Sodabeh Jahanbakhsh Godehkahriz, Ali Ebadi, Mohammad Sedghi,
Volume 7, Issue 2 (3-2021)
Abstract
Under drought stress condition, the signaling system induces expression of certain genes to counteract the deleterious effects of environmental stress. Among the essential micronutrients for plant growth and development, zinc has an important role in many plant metabolic processes including gene expression and stress tolerance. In order to investigate the effect of drought stress and ZnO on relative expression pattern of some genes involved in abiotic stresses (including WRKY1, HMA2 and ZIP1 genes) in wheat cultivars, a factorial experimental was conducted in pot condition based on a completely randomized design with three replications. In this experiment, the first factor was three levels of drought stress (35, 60 and 85% of field capacity), the second factor was three wheat cultivars (including Heidari, Meihan and Sysons), and the third factor was three levels of ZnO (0, 0.5 and 1 g/l-1). According to the results, with increasing the level of drought stress, the relative expression of WRKY1 and ZIP1 genes in drought tolerant cultivar (Meihan), and also with increasing nanoparticle concentration over stress time, the expression of ZIP1 gene in drought sensitive cultivar (Sysons) increased. The highest relative expression of HMA2 gene was observed in Heidari cultivar under mild drought stress. Generally, the expression of all three genes studied in tolerant cultivar (Meihan) increased under drought stress. Increasing the expression level of HMA2 and ZIP1 genes could be related to the transfer of zinc to consuming tissues and also, to increase the consumption of zinc in current metabolism of plant, which is important in tolerance of wheat to drought stress.
Mohaddaseh Gholami Farahabadi, Gholam Ali Ranjbar, Ali Dehestani-Kalagar, Nadali Bagheri,
Volume 8, Issue 1 (8-2021)
Abstract
Bread’s quality depends on wheat flours quality and quantity and for the goal to be achieved, the usage of suitable wheat varieties should be considered. Present study focuses on analyzing doubled-haploid lines of wheat’s bread backing quality and the relationship between qualitative traits and glutenins reservoir proteins. In current work, traits related to bread backing quality of 30 doubled-haploid lines of wheat including their parents and two control varieties (Ehsan and Morvarid) were evaluated. SDS-PAGE test was conducted to identify total amount of protein and the relationship between seeds reservoir proteins and qualitative traits, afterward, a test was conducted to evaluate expression of genes involved in bread backing quality. Results showed that there are significant differences on evaluated traits among all wheat’s genotypes. The highest volume of Zeleny sediment were related to DH-143 and DH-159 (34 and 31 ml, respectively), the highest amount of wet gluten were attributed to DH-159 and DH-143 (77.8 and 74.85 gr, respectively), the highest amount of dry gluten were attributed to DH-159 and DH-143 (26.21 and 25.11 gr, respectively), the highest amount of water absorption percentage were attributed to DH-159 and DH-143 (51.59 and 49.74%, respectively), and the highest percentage of protein content were attributed to DH-143 and DH-159 lines (with the amount of 18.03 and 17.72% respectively). Analyzing of bread backing quality traits indicated that DH-143 and DH-159 were better than the other genotypes. SDS-PAGE test results pointed that the highest amount of seed’s protein is attributed to DH-159 and DH-143 (28.23 and 26.63 µ/gr, respectively). Based on gene expression analysis (using real-time PCR), it was indicated that lines DH-143 and DH-159 had a higher level of expressed than the control treatments for HMW-X, HMW-Y and PDIL genes. Therefore, lines DH-143 and DH-159 could be used in breeding program for optimizing bread backing quality.
Reza Mir Derikvand, Seyede Sajad Sohrabi, Seyyed Mohsen Sohrabi, Kamran Samiei,
Volume 8, Issue 2 (3-2022)
Abstract
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