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Showing 3 results for Souri
Azadeh Souri, Asghar Mirzaie-Asl, Leila Khodaei, Mohammad Reza Abdollahi,
Volume 6, Issue 2 (3-2020)
Abstract
Autumn sowing of sugar beet is a suitable way in sustainable agriculture. Bolting is an undesirable phenomenon which reduces sugar beet yield and it is the most important limiting factor in autumn sowing of sugar beet. Identification and comparison of the sequence of flowering genes in various genotypes can help to understand the molecular mechanisms controlling bolting. In the previous studies, it was revealed that expression level of FT1 and VIN3 genes in sugar beet is associated with bolting resistance. In this study, the sequence of FT1 gene promotor and three versions of VIN3 gene promoters of sugar beet were compared in three bolting resistant and three bolting susceptible genotypes. Primer design for each gene was carried out using the DNA sequences found at the NCBI database. DNA was extracted from leaf samples growing in pots and was used as template in PCR reactions. Similar length of amplified fragments for each promoter gene in bolting susceptible and bolting resistant genotypes were selected and sequenced for more accurate assessment. There was no mutation in the FT1 gene promoter, however 624 substitution and insertion/deletion mutations were observed in the promoter of three versions of VIN3 gene. A 228-bp ins/del region was detected in the VIN3-like1 promoter. This region contains promoter elements and seems to have a control function. Comparison of detected mutations between susceptible and resistant genotypes did not show a distinct pattern for bolting.
Hossein Zeinalzadeh-Tabrizi, Sadollah Mansouri, Abbas Fallah-Toosi,
Volume 8, Issue 1 (8-2021)
Abstract
Analysis of genotype by environment interaction using different statistical methods is very important in plant breeding. In order to evaluate the seed yield stability of promising sesame lines using different parametric and non-parametric statistics, an experiment was conducted using 13 promising sesame lines with check variety Oltan at three locations of Karaj, Mashhad, and Moghan (Iran) in a randomized complete block design with four replications over two years (2016 and 2017). Combined analysis of variance for seed yield of promising sesame lines showed that the effect of genotype and the three-way interaction of genotype × year × location at the level of 0.01% probability were statistically significant. Karaj-96 environment with 1346 kg/ha and Mashhad-96 environment with 1001 kg/ha had the highest and lowest mean yield, respectively. The highest and lowest mean seed yield among genotypes in all test environments were related to G6 line with 1444 kg/ha and G12 line with 762 kg/ha, respectively. Heatmap along with cluster analysis divided both genotypes and stability parameters into three groups. Based on cluster analysis, genotype G12 was clustered into the first group, genotypes G1, G3, G7, G8, and G13 were clustered into the second group and the rest of the genotypes along with the check cultivar Oltan were clustered into the third group. The genotypes of the second group with the highest rank in most criteria of stability stasistics were stable compared to other genotypes and among them, the genotypes G8, G1 and G3 (with mean yields 1417, 1398 and 1291 Kg/ha, repectively) were selected and recommended in the test locations due to their average yield above the average yield of all genotypes.
Davood Kiani, Gholamreza Ghodrati, Sadollah Mansouri,
Volume 9, Issue 1 (9-2022)
Abstract
Sesame is an important crop plant for harsh environmental conditions because it is relatively resistance to drought stress. Evaluation of different genotypes in different climate condition plays a fundamental role in selection of the best genotypes before the commercial release of a variety and helping in identify plant traits that should be monitored during breeding experiments. In the present study, 10 promising lines obtained from the preliminary yield test were investigated to evaluate the yield compatibility along with 6 local cultivars in a randomized complete block design experiment with three replications in two cropping years (2018 and 2019) in Dashtestan climate condition in Bushehr province. During the growing season, phenology traits, grain yield components and grain yield were measured. Based on the results of ANOVA, statistically significant difference was observed between different genotypes for plant height, height of the first sub branch, height of the first capsule, number of sub branches, number of capsules per plant, length of capsule, length of capsule bearing zone, number of seeds in capsule and grain yield. Based on the mean comparison and biplot analysis the genotype 12 (Local Dashtestan), 2 (SES97-103), 7 (SES97-110) and 15 (Local Jiroft), were identified as superior genotypes for grain yield. Genotype 5 (SES97-105) and genotypes 14 (SES97-124) with 104.5 days and 4 (Local Darab1) with 111 showed the highest and lowest number of days to maturity, respectively. First capsule height showed the highest positive and significant phenotype (0.56) and genetic (0.78) correlation with grain yield. Days to the end of flowering and days to physiological maturity traits showed a negative genetic correlation with yield. Cluster analysis separated 16 sesame genotypes into four separate groups. Based on regression analysis, the height of the first capsule was identified as the most sensitive trait in predicting the yield of sesame genotypes in Dashtestan region in Bushehr province, which seems it can be considered during breeding programs.
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