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Showing 8 results for Bagheri
Saeed Bagherikia, Mohammadhadi Pahlevani, Ahad Yamchi, Khalil Zenalinezhad, Ali Mostafaie,
Volume 4, Issue 1 (9-2017)
Abstract
Under drought stress conditions, as one of the most important limiting factors of grain yield in wheat at arid and semi-arid regions, the remobilization of assimilates gain would be more valuable to grain filling. There are a few reports on the importance of remobilization of the root during the grain filling period under drought stress conditions. An advanced mutant line of bread wheat (T-65-7-1) along with its wild type (cv. Tabasi), were planted at two moisture conditions (normal and 30-40% of field capacity) as a factorial experiment based on a completely randomized design with three replications. Sampling for gene expression analysis was conducted from the root in two stages (7 and 21 days after anthesis). In these genotypes, fructan remobilization, efficiency of fructan remobilization, and relative expression of genes involved in the synthesis and hydrolysis of fructan during the grain filling period, in root, were studied under terminal drought stress. The results showed that the stored fructan in the root participated in the assimilate remobilization. Higher fructan remobilization through root to grain in mutant line under drought stress conditions was due to over-expression of genes involved in the synthesis of fructan (1-SST and 6-SFT) at 7-days after anthesis and in hydrolysis of fructan (6-FEH) at 21-days after anthesis, compared to wild type. Drought stress did not cause a significant change in gene expression of 1-FFT and 1-FEH genes in the root of both genotypes, which confirms the only β (2,6) linkages as predominant form of fructan has affected under drought stress conditions. In wheat breeding programs, 1-SST, 6-SFT and 6-FEH can be used as molecular markers for selecting genotypes with high fructan content and more remobilization.
Amir Mohammad Mahdavi, Nadali Babaeian Jelodar, Ezatollah Farshadfar, Nadali Bagheri,
Volume 7, Issue 1 (9-2020)
Abstract
In order to determine yield stability of 23 bread wheat genotypes and two commercial cultivars as check, an experiment was conducted based on a randomized complete block design with three replications in the experimental field of faculty of Agriculture, Razi University Kermanshah (Iran), during three cropping seasons (2015-2018). The results of combined ANOVA showed that the effect of environment, genotype and genotype × environment interactions on grain yield were significant (P<0.01). Stability was evaluated using environmental variance statistics, coefficient of variation, Wrick´s ecovalence, Shukla’s stability variance, Regression slope, deviation from regression slope, Plaisted and Peterson method and AMMI model. Variance analysis of additive main effects and multiplicative (AMMI) showed that three IPCAs were significant at 1% probability level. The first three principal components justified a round 85.7% of the sum of square of the interaction. Also, AMMI stability value (ASV) was used for simultaneously using information obtained from two significant components of AMMI. According to ASV index, genotypes Pishgam, Wc-4958 and Pishtaaz had the lowest ASV value and were known as the most stable genotypes. Genotypes Wc-4987, Wc-47615, Wc-47399 and Wc-47638 had the highest ASV value and distance from the center of Bi-plot. Therefore, Pishtaaz is one of the most stable genotypes due to having the first rank in terms of studied parameters as well as proper bakery properties and desirable drought resistance. In general, regarding to the climate change in the country, especially in the rainfed conditions and based on the above statistics and the biplots derived from AMMI analysis, the Wc-4958 line, with pishtaaz and Pishgam cultivars as stable and adaptable genotypes, are suggested to rainfed conditions on the studied area.
Fatemeh Darvishnia, Mohammadhadi Pahlevani, Khalil Zaynali Nezhad, Khosro Azizi, Saied Bagherikia,
Volume 7, Issue 1 (9-2020)
Abstract
In order to determine the most effective indices for quantifying drought tolerance and identify genotypes that are tolerant to water stress in bread wheat, 50 bread wheat genotypes were compared in a randomized complete block design with three replications under both the non-stress dry farming with two complementary irrigation and the water stress dry farming conditions in Khorramabad, Iran. Analysis of variance showed that there was a significant difference among the genotypes in terms of all of the traits except the number of spike per area. In this study, eight indices including: Stress Tolerance Index (STI), Geometric Mean Productivity (GMP), Mean Productivity (MP), Yield Index (YI), Harmonic Mean (HM), Yield Stability Index (YSI), Stress Susceptibility Index (SSI), and Tolerance index (TOL) were calculated by using of seed yield of the genotypes under both conditions. Indices that selection based on them will improve the yield in both conditions, are considered as suitable index. STI, GMP, MP and HM were introduced as suitable index for drought resistance selection. Genotypes Shiroodi and S-90-5 were determined as the most appropriate based on 3D plot. Based on positive correlation between water stress resistance indices and yield under stress and non-stress environments, STI and GMP were the best indices. By using the Biplot method, Shiroodi, S-90-5 and Oroum genotypes were considered as high yielding potential genotypes under the both conditions. According to the results of cluster analysis, genotypes were classified into three groups based on drought tolerance indices. Graphical analysis of genotypes also showed that genotypes Shiroodi and S-90-5 were more profitable than others under both drought stress and non-drought stress conditions. These genotypes could also be used as parents caring desirable genes in the crossing programs and selection of tolerate genotypes.
Mohaddaseh Gholami Farahabadi, Gholam Ali Ranjbar, Ali Dehestani-Kalagar, Nadali Bagheri,
Volume 8, Issue 1 (8-2021)
Abstract
Bread’s quality depends on wheat flours quality and quantity and for the goal to be achieved, the usage of suitable wheat varieties should be considered. Present study focuses on analyzing doubled-haploid lines of wheat’s bread backing quality and the relationship between qualitative traits and glutenins reservoir proteins. In current work, traits related to bread backing quality of 30 doubled-haploid lines of wheat including their parents and two control varieties (Ehsan and Morvarid) were evaluated. SDS-PAGE test was conducted to identify total amount of protein and the relationship between seeds reservoir proteins and qualitative traits, afterward, a test was conducted to evaluate expression of genes involved in bread backing quality. Results showed that there are significant differences on evaluated traits among all wheat’s genotypes. The highest volume of Zeleny sediment were related to DH-143 and DH-159 (34 and 31 ml, respectively), the highest amount of wet gluten were attributed to DH-159 and DH-143 (77.8 and 74.85 gr, respectively), the highest amount of dry gluten were attributed to DH-159 and DH-143 (26.21 and 25.11 gr, respectively), the highest amount of water absorption percentage were attributed to DH-159 and DH-143 (51.59 and 49.74%, respectively), and the highest percentage of protein content were attributed to DH-143 and DH-159 lines (with the amount of 18.03 and 17.72% respectively). Analyzing of bread backing quality traits indicated that DH-143 and DH-159 were better than the other genotypes. SDS-PAGE test results pointed that the highest amount of seed’s protein is attributed to DH-159 and DH-143 (28.23 and 26.63 µ/gr, respectively). Based on gene expression analysis (using real-time PCR), it was indicated that lines DH-143 and DH-159 had a higher level of expressed than the control treatments for HMW-X, HMW-Y and PDIL genes. Therefore, lines DH-143 and DH-159 could be used in breeding program for optimizing bread backing quality.
Esmaeil Dasturani, Khalil Zaynali Nezhad, Masood Soltani Najafabadi, Mohammadhadi Pahlevani, Hassan Soltanlo, Saeed Bagherikia,
Volume 8, Issue 1 (8-2021)
Abstract
The aim of this study was to determine the haplotype groups and identify the specific alleles associated with desirable agronomic characteristics in bread wheat. For this purpose, 42 local bread wheat genotypes belong to Iran region and nine commercial cultivars along with Chinese Spring variety (reference genotype) were cultivated in the format of augmented design and evaluated based on their 13 phenotypic traits. The results of descriptive statistics showed that awn length and day to flowering had the highest and lowest phenotypic coefficient of variation, respectively. Eight microsatellite markers were used to investigate the haplotype variation of QTLs associated with phenotypic traits located on wheat chromosomes 4B and 7D. The result showed that the genotypes were classified into 13 and 6 haplotype groups according to the allelic comparison with the reference genotype on chromosome 4B and 7D, respectively. In order to investigate the relationship between traits and markers, analysis of variance was performed based on completely randomized design with unequal numbers of replications for each marker. In general, of the 13 traits studied, there was a statistically significant linkage for eight traits and for the three traits, an allele-specific was introduced simultaneously. If the breeders are interested in genotype selection that simultaneously have three desirable characteristics such as early anthesis, semi-dwarfing and a greater number of grains per spike, they can use an allele-specific (153 bp) of Xgwm149-4B marker.
Jamshid Moradpour, Hadi Ahmadi, Mahmoud Bagheri, Daryoush Goudarzi,
Volume 9, Issue 1 (9-2022)
Abstract
Eggplant (Solanum melongena L.) has a high genetic variation in Iran and there are many landraces of this crop in Iran. In the present study, 15 superior genotypes of eggplant which were selected from Minab landraces accompanying two superior mother landraces (totally 17 lines) were studied for two successive years in three regions of Iran including Minab, Karaj and Jiroft. The experiment was conducted in Randomized complete block design with three replications. Finally, total yield of both years was measured and the combined analysis was done and the best line(s) for different climates were introduced using evaluation the stability of the lines via AMMI and GGE biplot procedures. Based on the results of means comparison of yield in the studied lines in each region from average of two years, GHE12 line in Minab region, SA13 line in Jiroft region and AM4, SA15 and SA5 lines in Karaj region have higher fruit yield than the other lines. Based on the results of yield comparison of the examined genotypes in each region from the average of two years of testing, GHE12 genotype in Minab region, SA13 genotype in Jiroft region and AM4, SA15 and SA5 genotypes in Karaj region had acceptable yield compared to other genotypes. However, according to the results of special adaptability and stability analysis, Y genotype for Minab region, SA13 genotype for Jiroft region and AM4 genotype for Karaj region are recommended
Fatemeh Keykha Akhar, Abdolreza Bagheri, Nasrin Moshtaghi, Masoud Fakhrfeshani,
Volume 9, Issue 1 (9-2022)
Abstract
have been bred with altered flower color using genetic engineering approaches. One of the most effective applications is the reduction of flower pigments by suppression of involved enzymes in their biosynthesis pathways. RNA interference (RNAi) has provided an effective tool for the knock down of genes involved in the production of flower pigments. In this study, a chi-RNAi construct was designed for chalcone isomerase (chi) gene to transform Petunia plants. Transgenic lines in one phenotype showed 5.6 fold reduction in chi expression in comparison to the control. Chalcone and naringenin were also extracted and quantified. A 24% reduction in naringenin content was obvious in all transgenic lines. Generally, the results of this research showed that RNAi technology can be used as an efficient method for silencing the flower pigments in petunia. In addition, the chalcone isomerase gene was identified as one of the effective genes in anthocyanin biosynthesis pathway in Petunia plants which is involved in the production of color in these plants; hence, chi gene silencing resulted in clear phenotypic alterations in this plant.
Mostafa Khodadadi, Behzad Sorkhilalehloo, Seyed Mohammad Mahdi Mortazavian, Jahangir Abbasi Kohpalekani, Mahmoud Bagheri, Milad Karbasi,
Volume 10, Issue 1 (9-2023)
Abstract
Eggplant is a highly nutritious vegetable that is widely consumed. The aim of this study was to evaluate the genetic diversity between eggplant accessions from the National Plant Gene-Bank of Iran. In the first year, a preliminary evaluation was conducted using 168 accessions. Based on preliminary evaluation results, 40 accessions were selected for complementary evaluation in the second year. The evaluation was based on 23 quantitative and qualitative traits. The results of the preliminary evaluation showed statistically significant (P<0.01) differences between accessions for all traits. Fruit shape frequencies were rounded (35.89 percent), elongated (32.18 percent), oval (13.67 percent), Semi-elongated (13.15 percent), and mace-shaped (5.11 percent). In the complementary evaluation, there were significant differences between accessions for all traits. Qualitative traits such as flower color (1.56) and fruit shape (1.53) exhibited the highest genetic variation, while fruit color (0.5) showed the lowest. Cluster analysis analysis results revealed four groups for accessions and the highest (22.34) and least (0.12) genetic distances between 1 and 2 and between 7 and 21accessions, respectively. Factor analysis showed that the first three factors explained 68.06 percent of total variation in data. The first and second factors were related to yield and yield components, respectively. Also, fruit yield traits showed high heritability and there was significant genetic correlation between these traits. Therefore, high heritable and high-scoring traits in these factors should be considered when selecting progenies in segregating populations for improvement in terms of fruit yield and shape.
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