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Showing 5 results for Ranjbar

Mohammad Reza Salavati Meybodi, Gholam Ali Ranjbar, Seyed Kamal Kazemitabar, Hamid Najafi Zarrini,
Volume 4, Issue 1 (9-2017)
Abstract

Water stress is one of the most common environmental stresses that limited tobacco production in IRAN. Identification of genetic diversity in tobacco germplasm and genetic relationships between traits help to improve tolerant varieties. In this study, the genetic diversity of 100 flue–cured tobacco (Nicotiana tabacum L.) cultivars was analyzed using 15 morpho-physiological traits and 25 ISSR primers. The cultivars were cultured in a simple lattice design (10×10) with two replications (with and without water stress) in Tirtash Tobacco Institute, Iran. Results showed relative water content (RWC) and cell membrane stability (CMS) in normal condition were significantly higher than stress condition. The estimated broadsense heritability was low for RWC and CMS that represents large environmental effects on these two traits. The results of genotypes clustering by UPGMA method with ISSR markers and by WARD method with morph-physiological traits did not match. Primer UBC814: (CT) 8A with 16 polymorphic bands of the 17 bands, had higher resolution than other primers and seems appropriate for molecular diversity studies in tobacco. The K394 genotype was identified as well as drought tolerant varieties. We can use results of this study for selecting genotypes with great genetic differences and chose the desirable traits and use in breeding programs for producing high heterosis hybrid with tolerance to drought stress in tobacco.
Shaghayegh Mehravi, Gholam Ali Ranjbar, Hamid Najafi-Zarrini, Ghader Mirzaghaderi,
Volume 7, Issue 1 (9-2020)
Abstract

Anise is an annual plant belonging to Apiaceae family and fruit essential oil of this herb is used in various pharmaceutical, cosmetic and beverage industries. Drought stress is one of the most critical limiting factors for anise production in worldwide. In this research, to finding interrelationships among different traits and performance, some anise genotypes were evaluated using the biplot method. In this study, 18 anise genotypes were evaluated in normal irrigation regime and drought stress conditions according to a randomized complete block design with three replications at the field of the Western Australia University (UWA), Australia. Fifteen phonological, morphological and physiological traits were measured. Results indicated the positive and significant correlations between yield and fruit number and fruit thousand weight in both conditions. Due to the negative correlation between phonological features with fruit yield in two different irrigation conditions, it could be concluded that to have genotypes with high fruit yield, selection for early ripening genotypes should be done in anise. In this study, a significant correlation was observed between fruit yield and relative water content in the stress condition. Therefore, this trait can be used as a physiological index to evaluate drought tolerance in anise. According to cluster analysis based on the measured traits, genotypes were divided into 3 groups in both non-stress and stress conditions. According to the results of the comparison of the means of the groups in non-stress and drought stress conditions, genotypes No. 1, 5, 6, 11, 12, 14, 15 and 16 were identified as the most drought-tolerant genotypes. These genotypes could be utilized in breeding programs for further improvement of drought tolerance in anise germplasm.

Mohaddaseh Gholami Farahabadi, Gholam Ali Ranjbar, Ali Dehestani-Kalagar, Nadali Bagheri,
Volume 8, Issue 1 (8-2021)
Abstract

Bread’s quality depends on wheat flours quality and quantity and for the goal to be achieved, the usage of suitable wheat varieties should be considered. Present study focuses on analyzing doubled-haploid lines of wheat’s bread backing quality and the relationship between qualitative traits and glutenins reservoir proteins. In current work, traits related to bread backing quality of 30 doubled-haploid lines of wheat including their parents and two control varieties (Ehsan and Morvarid) were evaluated. SDS-PAGE test was conducted to identify total amount of protein and the relationship between seeds reservoir proteins and qualitative traits, afterward, a test was conducted to evaluate expression of genes involved in bread backing quality. Results showed that there are significant differences on evaluated traits among all wheat’s genotypes. The highest volume of Zeleny sediment were related to DH-143 and DH-159 (34 and 31 ml, respectively), the highest amount of wet gluten were attributed to DH-159 and DH-143 (77.8 and 74.85 gr, respectively), the highest amount of dry gluten were attributed to DH-159 and DH-143 (26.21 and 25.11 gr, respectively), the highest amount of water absorption percentage were attributed to DH-159 and DH-143 (51.59 and 49.74%, respectively), and the highest percentage of protein content were attributed to DH-143 and DH-159 lines (with the amount of 18.03 and 17.72% respectively). Analyzing of bread backing quality traits indicated that DH-143 and DH-159 were better than the other genotypes. SDS-PAGE test results pointed that the highest amount of seed’s protein is attributed to DH-159 and DH-143 (28.23 and 26.63 µ/gr, respectively). Based on gene expression analysis (using real-time PCR), it was indicated that lines DH-143 and DH-159 had a higher level of expressed than the control treatments for HMW-X, HMW-Y and PDIL genes. Therefore, lines DH-143 and DH-159 could be used in breeding program for optimizing bread backing quality.

Seyede Sharare Arianezhad, Hamid Hamid Najafi Zarini, Mehdi Ghaffari, Gholamali Ranjbar,
Volume 9, Issue 1 (9-2022)
Abstract


This research was carried out to estimate the genetic variance components for sunflower fatty acids in two separate optimum and drought stressed conditions in Karaj during 2020 and 2021 growing seasons. The plant materials consisted of 12 hybrids derived from crossing of four restorer lines by three cytoplasmic male sterile lines (Testers) that were evaluated in two separate experiments as randomized complete block design with three replications. Drought stress made a reduction in oil yield (34 percent), oil content (six percent), stearic acid (4.7 percent) and oleic acid (10.6 percent) and an increase in palmitic acid (12 percent) and linoleic acid (2.8 percent). Line × tester interaction effect had a major role in explanation of the variance of the hybrids in terms of fatty acid content in both conditions, indicating the critical role of non-additive effects in genetic control of these traits. Under optimum irrigation, oil yield, oil content and stearic acid content were under control of both additive and dominant gene action and palmitic, oleic and linoleic acids were under control of dominant gene action. Under drought stress, except oil content which was under control of additive effects, all the other traits were under control of dominant gene action. According to the results of this study, fatty acid composition of sunflower was under control of non-additive genetic effects and the crossing-based methods and hybrid breeding could be used for improvement of sunflower in terms of fatty acid composition.

Fariba Ranjbar, Babak Abdollahi Mandoulakani, Raheleh Ghasemzadeh,
Volume 10, Issue 1 (9-2023)
Abstract

To evaluate the expression pattern of genes encoding antioxidant enzymes catalase, ascorbate peroxidase and polyphenol oxidase under iron deficiency conditions in Fe- efficient (Pishtaz) and -inefficient (Falat) bread wheat cultivars, a CRD (completely randomized design) based factorial experiment was conducted with three replications. The cultivars were grown under iron deficiency (Less than 1.5 mg Fe/kg soil) and compared with normal conditions (10 mg Fe/kg soil). The relative expression levels of the above-mentioned genes were measured using Real-time PCR technique in the leaves and roots of the cultivars at two growth stages: vegetative (one month after germination) and reproductive (30% of heading). The results revealed a remarkable enhancement in calatalse expression in the roots of both cultivars in the vegetatative stage but it was higher in Fe-efficient cultivar than -inefficient one. The expression of this gene was decreased in leaves at the same stage as well as in the roots of both cultivars in the vegetative stage. The expression level of ascorbate peroxidase gene in the reproductive stage in the roots of Fe-inefficient cultivar was higher than that of -efficient one. In the vegetative stage, the expression of this gene increased in the leaves and roots of Fe-efficient cultivar, but it was decresed in Fe-inefficient cultivar. The relative expression level of polyphenol oxidase gene in the vegetative stage under iron deficiency conditions in the leaf increased almost three times, compared to the roots, while the expression of this gene decreased in the reproductive stage in both leaves and roots. By increasing the expression of both catalase and ascorbate peroxidase genes in the roots of both cultivars in the reproductive stage under iron deficiency conditions, it seems that bread wheat cultivars might reduce the deletrious effects of stress and maintain yield through transferring much iron to the seeds in the seed filling stage. The findings of the present study may increase our understanding of the important role of genes encoding antioxidant enzymes in Fe deficiency stress conditions.


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