[Home ] [Archive]   [ فارسی ]  
:: About :: Main :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..



 
..
:: Search published articles ::
Showing 1 results for Darvishian

Ali Darvishian, Ahmad Ismaili, Farhad Nazarian-Firouzabadi, Reza Mirdrikvand, Tahmasb Hosseinpour,
Volume 2, Issue 2 (3-2016)
Abstract

Plant breeding is selection of advanced genotypes and its progress depends on correct evaluation of genetic variation. Among different selection procedure, molecular markers have a good potential for evaluation of variation. In this research, RAPD molecular markers were used to evaluation of genetic diversity among 25 wheat cultivars and advanced breeding lines. Genomic DNA was extracted from leaves by Dellaporta method and 30 primers were used for PCR amplification. Results of Primers led to 200 storable electrophoretic bands which 130 of them (65%) were polymorphic. F4 and A18 primers produced the greatest and lowest band, respectively. Cluster analysis was performed based on band presence (1) and absence (0) using Jaccard coefficient similarity and UPGMA method. Similarity coefficient ranged from 0.22 to 0.87 with an average of 0.64. The highest similarity (0.87) was observed between Azar2 and Sardari and lowest similarity (0.22) was observed between Seimareh and BAVICORA. With cut of line on 0.72 in dendrogram, 6 main groups were clustered and other genotypes were clustered in different group. Regarding to the high similarity among these genotypes, it is necessary to develop the wheat germplasm in related research centers.

Page 1 from 1     

پژوهش های ژنتیک گیاهی Plant Genetic Researches
Persian site map - English site map - Created in 0.06 seconds with 27 queries by YEKTAWEB 4642