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Showing 38 results for Marker
Mahdi Rezaei, Abdoreza Kavand,
Volume 7, Issue 2 (3-2021)
Abstract
Cultivar identification in micro-propagated date palm seedlings is laborious so that application of molecular markers to facilitate and acceleration of the procedure seems inevitable. Given the need for control the originality of micro- propagated date palm seedlings, the aim of this study was evaluation of SSR markers usability to cultivar identification in micro-propagated date palm seedlings. Original samples of Green Ghanami, Red Ghanami, Gantar, Deiry, Ostaemran, Barhi, Medjool, Zahedi and Piarum cultivars were used control. Taking into account the rigidity of leaves and subsequently high consumption of liquid nitrogen to powder leaves, an efficient method for powdering of leaves using Tissue Lyser II instrument was optimized. Eight SSR primer pairs were used for polymerase chain reaction. The Results showed that by using these molecular markers and reliable controls, determination of micro- propagated date palm cultivars is feasible. Clustering of cultivars showed that all of them were differentiated using five SSR primer pairs including mPdCIR025, mPdCIR057, mPdCIR070, PDAG1003 and DP175. Also, barcoding of scored band illustrated that c1 allele (230 to 240 bp) for Piarum cultivar and d3 allele (220 to 230 bp) for Medjool cultivar were exclusive. Totally to make the results referable, cultivar identification diagram was drawn up.
Abouzar Abouzari, Ahmad Reza Dadras, Behrouz Golein, Yahya Tajvar,
Volume 7, Issue 2 (3-2021)
Abstract
In breeding programs, it is necessary having knowledge of the relatedness and genetic diversity in germplasm pools. The spread of cultivated regions and the high levels of production indicates citrus importance in the global economy. Therefore, 110 citrus genotypes were evaluated using 12 ISSR markers. Overall, 154 polymorphic bands were scored with an average of 12.8 alleles per primer. The polymorphism percentage ranged from 57 for the ISSR1 to 82 for the ISSR9. Averages of polymorphic information content (PIC), marker index (MI), gene diversity index (Nei), Shannon index (I) and number of effective alleles (Ne) were 0.48 ± 0.002, 6.14 ± 1.17, 0.42 ± 0.11, 0.61 ± 0.12 and 1.78 ± 0.27, respectively. Based on genetic diversity statistics, the studied population had high genetic diversity, and four markers (ISSR11, ISSR9, ISSR4, and ISSR5) had more potential for differentiation of genotypes. Cluster analysis and model-based structure analysis, divided the genotypes into five groups and four subpopulations based on the Neighbor-Joining method (NJ) and Bayesian approach, respectively. Based on both analyses, grouping of unknown genotypes and control cultivars in the same group probably confirms the assumption of a common genetic background between these genotypes. Results from the two analyses showed that Pummelo (C. maxima), Mandarin (C. reticulate), and Citron (C. medica), as three true citrus species, separated in different groups. In addition to the three true species, at least one species or another genus of citrus relatives is involved in the genetic makeup of the studied population. In this study, although both used analyses were effective in completing each other's information, by considering the degree of genetic mixing and the information of the origin of the genotypes, the effectiveness of model-based structure analysis in evaluating genetic relationships could be achieved.
Ghasem Eghlima, Azizollah Kheiry, Mohsen Sanikhani, Javad Hadian, Mitra Aelaie,
Volume 8, Issue 1 (8-2021)
Abstract
Twenty-two G. glabra populations were used to study the genetic diversity of ISSR molecular markers. 12 primers were used to amplification of genomic DNA fragments of G. glabra populations. High genetic diversity based on ISSR markers was observed among individuals. A total of 130 bands were formed and 105 bands were polymorphic. The mean polymorphism percentage among studied populations was 80.47. The highest polymorphic percentages were assigned to IS23, IS21, IS9, IS13 and IS15 primers. The mean of PIC and MI were 0.347 and 2.47, respectively. The Shannon index (I) varied between 0.207-0.393 and the Nei genetic variation index (h) from 0.140 to 0.026. Darab and Solataniyeh populations showed the lowest and highest genetic diversity, respectively. The percentage of polymorphic loci was varied between 35.224 to 65.71%. The observe allele number and effective alleles number was 1.46 and 1.34, respectively. Based on the genetic distance Nei, populations Bardsir and Baft had the highest genetic similarity (0.888) and populations Bardsir and Solataniyeh had the least genetic similarity (0.132). The studied populations were grouped into three main groups by cluster analysis using UPGAM and Jaccard's similarity coefficient. The results showed that the ISSR marker is a reliable marker system for revealing a high level of polymorphism and can be used to study genetic diversity and further examinations as a subset of breeding programs in G. glabra.
Saeid Navabpour, Ahad Yamchi, Sasan Golcheshmeh,
Volume 8, Issue 1 (8-2021)
Abstract
The present study was performed to classify and study genetic diversity between Calotropis procera accessions from different regions of Kerman province (Iran) using ISSR markers. In total, DNA from 14 plant samples with nine ISSR primers was amplified by PCR and their banding pattern was obtained. The primers showed acceptable polymorphism (35.93) and minimum and maximum polymorphic information content (PIC) of primers in this study were 0.11 for ISSR9 primer and 0.41 for ISSR3 and ISSR8 primers, respectively. Genetic similarity based on Nei index was varied from 0.405 to 0.745 and the lowest genetic similarity was found between J3 (Related to Jiroft) and D2 (Related to Dosari) and the highest genetic similarity was found between J1 and J2 (both of them for Jiroft). By using UPGMA cluster analysis, samples divided into four groups, and the second and third groups contained more accessions. In terms of genetic similarity, two accessions of Jiroft 1 (J1) and Jiroft 2 (J2) which classified in the same cluster were closer. Also, the accessions collected from Anbarabad were at a longer genetic distance than other accessions. Principal coordinate analysis also showed that the first and second components justify 67 percent of obtained genetic diversity. In general, ISSR markers were useful for classifying Calotropis procera accessions and according to the obtained information about existence of genetic diversity between Calotropis procera accessions of Kerman province, this diversity could be useful in the future for breeding and production of Calotropis procera.
Fatemeh Raeisi, Leila Fahmideh, Barat Ali Fakheri, Mojtaba Kikhasaber,
Volume 8, Issue 1 (8-2021)
Abstract
Due to the importance of date palm production in Iran, introduction of new cultivars, improvement of current cultivars and studying the genetic diversity among available cultivars is essential for improvement the quantity and quality of date palm production. In this research, 15 different local genotypes of date palm collected from Saravan, Jalgh, Nahook and Sinokan regions of Sistan and Baluchistan province were used for diversity analysis. To this end, DNA was extracted from leaves using Delaporta method and DNA quality and quantity were determined using spectrophotometer and 1% gel electrophoresis. PCR was performed using specific rbcL primers under determined conditions and the amplicons were sequenced. To study the relationships and genetic distances between genotypes, the results of sequencing were analyzed and dendrogram of phylogenetic relationships as well as sequence similarity matrix were generated using Bioedit, and MEGA7 software. The results of the present study showed that there were a total of 553 different residues for this marker of which 505 residues contained deletion and addition, and 48 residues were without deletion and addition. The genetic distance ranged from 0.0 to 0.037 and the highest intra-regional diversity was related to cultivar Jm13_sabzoo. Based on the dendrogram obtained from cluster analysis, the studied cultivars were divided into two branches, in which the first branch contained cultivar Sabzoo from Jalgh region and the other cultivars were grouped in different sub-branches of second branch. Although the rbcL marker is useful for studying and recognizing diversity of intraspecific relationships, a low genetic distance was estimated for the studied date palm genotypes. However, it is suggested that the other DNA barcodes as well the other appropriate molecular markers could be used for future studies of date palm genetic diversity.
Esmaeil Dasturani, Khalil Zaynali Nezhad, Masood Soltani Najafabadi, Mohammadhadi Pahlevani, Hassan Soltanlo, Saeed Bagherikia,
Volume 8, Issue 1 (8-2021)
Abstract
The aim of this study was to determine the haplotype groups and identify the specific alleles associated with desirable agronomic characteristics in bread wheat. For this purpose, 42 local bread wheat genotypes belong to Iran region and nine commercial cultivars along with Chinese Spring variety (reference genotype) were cultivated in the format of augmented design and evaluated based on their 13 phenotypic traits. The results of descriptive statistics showed that awn length and day to flowering had the highest and lowest phenotypic coefficient of variation, respectively. Eight microsatellite markers were used to investigate the haplotype variation of QTLs associated with phenotypic traits located on wheat chromosomes 4B and 7D. The result showed that the genotypes were classified into 13 and 6 haplotype groups according to the allelic comparison with the reference genotype on chromosome 4B and 7D, respectively. In order to investigate the relationship between traits and markers, analysis of variance was performed based on completely randomized design with unequal numbers of replications for each marker. In general, of the 13 traits studied, there was a statistically significant linkage for eight traits and for the three traits, an allele-specific was introduced simultaneously. If the breeders are interested in genotype selection that simultaneously have three desirable characteristics such as early anthesis, semi-dwarfing and a greater number of grains per spike, they can use an allele-specific (153 bp) of Xgwm149-4B marker.
Mahmood Aslanparviz, Varahram Rashidi, Mansour Omidi, Alireza Etminan, Alireza Ahmadzadeh,
Volume 8, Issue 2 (3-2022)
Abstract
Evaluation of genetic diversity is the key principal for plant breeding, providing an opportunity to discover novel characters and alleles for breeders. In the present study, 69 durum wheat genotypes were investigated for genetic diversity using several inter-simple sequence repeat (ISSR) markers. Sixteen ISSR primers amplified a total of 163 fragments, which out 160 fragments were polymorphic. The mean values of polymorphic information content (PIC), resolving power (Rp) and marker index (MI) indicated that the used ISSR primers could be exploited for further assessing relationships among investigated genotypes and population structure analysis. The results of the molecular analysis of variance showed that the genetic variation within populations is more than between them. Based on genetic variation parameters, the highest number of observed alleles (Na), Shannon’s information index (I) and the percentage of polymorphic loci (PPL) were found in Iranian landraces. Cluster analysis and population structure grouped all investigated genotypes into three main clusters and six subpopulations, respectively. In conclusion, our results revealed the high rate of genetic diversity within Iranian landraces, so this germplasm can be used as a valuable gene source for the selection of parent lines and use of them in durum wheat breeding programs.
Soheila Afkar, Faranak Hadi, Ali Ashraf Jafari,
Volume 8, Issue 2 (3-2022)
Abstract
Festuca is one of the largest genera of the grass family, which has more than 600 species with different ploidy levels. The aim of this study was to estimate the genetic diversity within 22 populations of three species of Festuca (Festuca arundinacea, F.rubra and F.ovina) using a seed storage protein electrophoresis pattern. These species showed a significant variation in the number of protein bands from 5-13. The highest number of bands was found in G17 (F.rubra) and the lowest number of protein bands was in G5 (F.ovina). Band number 14 was only observed in G3. It is suggested that this band can be considered as a specific band for the identification of this genotype. According to the results of AMOVA analysis, there is a high level of genetic diversity within the species rather than between species that can be due to the out-crossing nature of this genus. According to observed differences for variation parameters among the three studied species, it is concluded that they have dissimilar genetic structures. The results of cluster analysis based on seed storage protein profiles in evaluated genotypes using Euclidean distance matrix and UPGMA method showed four groups. The lowest similarity coefficient was between G14 and G15 (F.arundinacea) with G6 (F.ovina). Hence, it is suggested that they evolved from a different evolutionary process and it is suggested to use them as the parents of new synthetic varieties. The observed diversity in the seed protein pattern in the three species of Festuca, can be explained by allogamy-induced-heterozygosity, species difference or population collection from various regions.
Alireza Asghari Mirak, Seyed Siamak Alaviakia, Seyed Abolghasem Mohammadi,
Volume 9, Issue 1 (9-2022)
Abstract
Henbane has a high medicinal value due to the presence of hyoscyamine and scopolamine alkaloids. Improving the quality and quantity of henbane alkaloids using modern breeding methods requires evaluating the genetic diversity. The genetic diversity of henbane has been investigated using morphological, biochemical and molecular markers in several studies and the superiority of molecular markers over other markers has been proven. To this end, in 2018, the genetic diversity of 96 henbane genotypes collected from the habitats of northwest Iran was investigated using IRAP and REMAP molecular markers. For IRAP markers, out of 36 possible combinations obtained from eight LTR primers, seven combinations had a fine and scalable amplification. In the REMAP technique, the combination of 11 ISSR primers with eight LTR primers was used, and 12 combinations could be scored out of 88 possible combinations. The average amount of polymorphic information for IRAP and REMAP markers was 0.30 and 0.32, respectively, and the average marker index for these two markers was estimated as 2.59 and 2.47. Based on these criteria, REMAP marker was more efficient than IRAP in estimating the genetic diversity of henbane. In the analysis of molecular variance using IRAP and REMAP markers, intra-population variability was estimated to be higher than inter-population, which indicates the high diversity of these populations in northwestern Iran. Cluster analysis based on IRAP marker failed to separate species and populations, but REMAP marker was able to separate H. pusillus and H. reticulatus species to a high degree. A high shannon index in this research suggests that IRAP and REMAP retrotransposon markers resulted in a high genetic diversity within henbane populations with a high insertion in the genome of henbane populations.
Razieh Ghorbani, Raheleh Ghasemzadeh, Hadi Alipour,
Volume 9, Issue 1 (9-2022)
Abstract
In order to identify loci controlling seedling morpho-physiologic characteristics in 88 bread wheat cultivars, a greenhouse experiment based on simple alpha lattice was conducted under both normal and 120 mM (12 ds/m) salt stress condition of the Faculty of Agriculture, Urmia University in 2020-2021 cropping season. Chlorophyll a, b and carotenoid content, proline, plant fresh and dry weight, plant height and leaf relative water content (RWC), Na+, K+ and K+/Na+ concentrations were measured. After genotyping by sequencing with Ion Torrent technology and removal of SNPs with more than 20% of missing data and minor allele frequency less than 5%, a total of 5869 SNP markers were identified. Based on association mapping with the mixed linear model (MLM) method, a total of 25 marker-trait associations were detected under normal conditions. The A and D genomes had the highest and lowest number of significant marker-trait associations (MTAs). Among the studied traits under normal conditions, chlorophyll a had the highest number of MTAs on 1A, 3B, 3D, 5B, 7A chromosomes with eight MTAs. A total of 21 MTAs were identified under salt stress conditions which the genome B and D had the highest and lowest number of MTAs, respectively. Five MTAs were identified for plant fresh weight, which were located on chromosomes 4A and 6B. The results of this study provide valuable information about the loci associated with the studied traits, which can be used in marker assisted selection in wheat breeding programs after confirmation in biparental populations and additional experiments.
Halime Arbabi, Mojtaba Keykhasaber, Leila Fahmideh, Valiollah Ghasemi Omran,
Volume 9, Issue 2 (3-2023)
Abstract
Luffa (Luffa cylindrica) is a plant from the Cucurbitaceae family that grows mostly in tropical and subtropical regions, as well as in most regions of Iran. In this research, the genetic diversity of nine native and non-native genotypes of L. cylindrica was investigated through the evaluation of the chloroplast trnH-psbA intergenic region (IGS). After sampling the young leaves, DNA extraction was performed by using the Dellaporta method, and PCR was conducted by using IGS intergenic region primers. After sequencing of the amplified products, their quality was determined using Chromas software and then aligned using ClustalW method by BioEdit and MEGA7 softwares. Next, the dendrogram of phylogenic relationships was drawn and the matrix of the difference and similarity of the sequences were determined. In the present research, by analyzing the relationships between studied samples, based on the trnH-psbA (IGS) marker, a strong intraspecies variation was observed in native and non-native L. cylindrical genotypes. The genetic distance matrix between the samples examined in this research ranged from 0 to 6.865 with an overall average distance of 2.53. The average value of synonymous and non-synonymous substitutions (dN/dS) for the IGS sequence was ds/dn = 0.68, which indicates positive and pure line selections in the process of natural selection of studied genotypes. The results of this research showed that trnH-psbA is a suitable marker for evaluation of the intraspecific diversity of luffa species.
Hossein Abdi, Hadi Alipour, Iraj Bernousi, Jafar Jafarzadeh,
Volume 10, Issue 1 (9-2023)
Abstract
Evaluating the population structure is essential for understanding diversity patterns, choosing proper parents for crossing, accurate identification of genomic regions controlling traits, and evolutionary and kinship relationship studies. In this research, the genetic structure of a wheat population was studied in a panel consisting of 383 Iranian wheat genotypes of hexaploid (cultivars and landraces) and tetraploid species based on distance-based methods (principal component analysis and discriminant analysis of principal component). For this purpose, 16270 single nucleotide polymorphism (SNP) markers obtained by the GBS technique were used. According to the results, almost a quarter of the total variance was belonged to the diversity between populations, and the Fst coefficient between cultivars and landraces was equal to 0.15. In contrast, the above coefficient between tetraploid samples and hexaploid landraces was high and equal to 0.44. Genome D had the lowest value of Fst index and chromosome 4B showed the highest Fst coefficient, and other genetic diversity indices. Although the PCA biplot distinguished hexaploid wheat cultivars from landraces, it was unable to distinctly separate tetraploid genotypes from other genotypes. Accurate evaluation of the population structure with the DAPC method was able to identify and separate the predetermined successfully groups, suggesting that the DAPC approach maximizes the differentiation between groups and minimizes the changes within the group. Partial admixture between cultivars and landraces of hexaploid wheat can be related to gene exchange between these two groups or perhaps their wrong labeling at the time of collection. In general, the results of this study provided valuable information about the genetic differentiation of Iranian tetraploid and hexaploid wheat, which can be used in future wheat breeding programs. Further, protecting these genotypes in gene banks is necessary for different strategies.
Maryam Ghorbani, Kianoosh Cheghamirza, Saeed Abbasi, Zahra Aziziaram,
Volume 10, Issue 1 (9-2023)
Abstract
The current research was carried out to evaluate genetic diversity of 18 common bean cultivars and promising lines and to determine SSR and SCoT informative markers associated with 14 seed characteristics including the number of seeds per pod, 100 seed weight, seed length, seed width, the contents of crude protein, total soluble sugar, starch content, crude fat, iron, calcium, magnesium, zinc, uronic acid, and mineral ash. The polymorphism information content (PIC) values varied from 0.2 to 0.5 with an average of 0.39 for the SSR markers and from 0.19 to 0.42 with an average of 0.34 for the SCoT markers. The total average resolving power of SSR and SCoT markers were 1.54 and 5.34, respectively, indicating higher efficiency of SCoT markers than SSR markers for the diversity analysis. The common bean studied genotypes were clustered into three distinct groups for both markers based on the Complete Linkage method. Principal coordinate analysis (PCoA) for the SSR markers revealed that the first two principal components justified 59.05% of whole variation. For the SCoT marker, on the other hand, the fraction of variances explained by the first two principal components was equal to 25.43 indicating a better distribution of SCoT markers than SSR markers in the common bean genome. Analysis of molecular variance based on the grouping obtained from cluster analysis showed that the diversity within the group based on SSR and SCoT markers was equal to 89 and 78%, respectively. The results of regression analysis for the studied markers and the bean seed characteristics showed the existence of a significant relationships between a single marker with the several studied traits. This could indicate the association or linkage of the marker locations. Twelve out of 14 of the investigated common bean seed characteristics showed a significant relationship with at least one molecular marker.
Ramezanali Pourali, Mohammadhadi Pahlevani, Khalil Zeinalnejad,
Volume 10, Issue 2 (2-2024)
Abstract
Increasing grain yield and improving the quality of bread are among the most important goals of wheat breeding programs in Iran. Understanding the genetic control of traits and finding molecular or morphological markers associated with them are also prerequisites for any genetic engineering program. In this study, 100 progenies of a 10 × 10 diallel cross were used to analyse the genetics of grain yield and bakery values using STS markers associated with HMWG subunits. This research was carried out during 2018 and 2019 cropping season at Gorgan University of Agricultural Sciences and Natural Resources experimental fields. In the first year, 10 wheat cultivars, including Gonbad, Morvarid, Kalate, Ehsan, Sirvan, Baharan, Chamran2, Shush, Mehrgan and Brat collected from different geographical regions of Iran were planted and crossed in the field. In the second year, the parents and crosses were planted in the form of a randomized complete block design with three replications. The grain yield, number of spikes per plant, number of seeds per spike, seed weight, days to emergence and plant height were recorded. The results of this study indicated significant genetic differences between the parents. Narrow-sense heritability analysis revealed that the crossing of cultivars is the best breeding method to enhance seed yield, number of spikes per plant and days to emergence. Also, to improve the number of seeds per spike, seed weight and plant height, classical breeding methods may offer higher efficiency. Marvarid and Gonbad were ranked 1st and 2nd, respectively with respect to general combining abilities for grain yield, attributed to their positive and significant general combining ability effects. The highest specific combining ability was observed for Ehsan×Gonbad, Marvarid×Chamran 2 and Shush×Sirvan crosses. The results of molecular markers analysis showed that the STS markers were able to identify the difference in the baking value of cultivars. The quality score of the cultivars ranged 6 and 10 and to this end, Kalate and Brat were the top cultivars. Therefore, due to superiority in terms of both quantity and quality for yield, these cultivars can be used as parents with desirable genes for future breeding programs. Overall, the STS markers employed in this study proved to be valuable markers for enhancing the genetic background of bread wheat, particularly when employing marker-assisted selection for bakery value.
Nasrin Akbari, Siamak Alavi Kia, Mostafa Valizadeh,
Volume 10, Issue 2 (2-2024)
Abstract
Due to world population incline and the increasing wheat consumption as human main staple food, as well as high amount of waste of bread which is mainly due to its low quality, the wheat breeding programs to improve bread quality are of great importance. Therefore, evaluating the wheat grains quality and the genetic variation of bread-making quality traits among lines derived from crosses becomes imperative. To this end, the gliadin protein banding pattern of 28 recombinant inbred lines, their corresponding parents and 10 other commercial cultivars were examined via A-PAGE method. The variation between and within the lines and cultivars was determined using AMOVA according to the protein bands. The results of this study revealed high variation for gliadins coding loci with total mean of 73.96%. The percentage of polymorphism was estimated to be 91.67 and 56.25 for lines and commercial cultivars, respectively. The minimum and maximum number of gliadin bands were 12 and 25 bands, respectively. Also, based on PhiPT statistics, the significant difference was observed (P<0.05) between commercial cultivars and recombinant inbred lines in terms of gliadin banding patterns. Cluster analysis and PCoA via banding pattern of gliadins led to formation of three and four distinct groups, respectively. The highest variation was observed in ω-gliadins, suggesting that they may have a role in observed variation among genotypes and their bread making-quality traits.
Ms Sahar Amiri, Hooman Salari, Alireza Etminan,
Volume 11, Issue 1 (9-2024)
Abstract
Tomato is the second important vegetable in the world. Therefore, disclosure the germplasm variation as a precursor for its breeding is highly valuable. The study was conducted to assess the genetic diversity in a collection of tomato germplasm including previously cultivated cultivars, currently being cultivated and promising tomato lines in Iran. Polymorphism analysis between cultivars was performed via 20 CBDP primers. The primers amplified 406 genomic fragments, of which 215 bands showed polymorphism and the average percentage of polymorphism was 51%. The average index of polymorphic information content (PIC) and resolving power (Rp) were estimated as 0.30 and 4.49, respectively, which indicated the relatively appropriate efficiency of the primers' used. The genetic distance between the pairs of studied cultivars according to the Jaccard Coefficient ranged from 0.16 to 0.54 and its average was estimated as 0.38. The lowest and highest genetic distance were observed in Matin and Sana with a value of 0.16 and Hypeel 303 and 693 with a value of 0.54, respectively. Cluster analysis based on the Jaccard distance coefficient and the Neighbor Joining algorithm divided the cultivars into five groups. It is relatively consistence with the results of the analysis to the principal coordinates which showed that the first two components explained 15.04% of the total changes. In conclusion, this research showed that there is to some extent low genetic diversity among the Tomato's cultivars in Iran, while the primers which used revealed the polymorphism between them competently and separated them thoroughly. Based on the indices calculated to evaluate the efficiency of primers, CBDP18 and CBDP12 primers showed better efficiency in differentiating the studied genotypes. Based on these results, the use of diverse genetic resources and development of new varieties is necessary to mitigate vulnerability and the risk of epidemic disease such as fungal pathogens in tomato cultivation.
Smaeil Talebi Kouyakhi, Bahram Maleki Zanjani, Mostafa Modarresi, Alireza Tarang,
Volume 11, Issue 1 (9-2024)
Abstract
Food security is affected by several factors,one of the most important of which is the in the quantitative and qualitative increase of agricultural products. For the reason the role of rice in the daily nutrition of Iranians and the need to increase the efficiency of limited territorial resources, we are forced to increase the yield per unit area. Identifying genotypes that contain alleles of genes related to yield improvement is one of the methods of rice plant breeding so that these genotypes, so that these genotypes can be used in breeding programs, to produce high-yield varieties, in plant breeding programs to produce high-yielding cultivars. Considering the importance of the number of seeds in the panicle and its effect in increasing the yield per unit area, in this research, among several genes related to yield, the screening of rice genotypes was investigated using on the functional marker related to the Gn1a gene. In this research, 52 local and improved genotypes of rice were prepared from the collection of the Rice Research Institute of Iran and the grain number trait was investigated based on phenotypic and molecular evaluations in the field related to the band pattern created by a specific primer pair Grain number 1a gene (Gn1a) contiguous with the gene location controlling the grain number trait. The result of the genetic evaluation led to the identification of 15 cultivars with the allele of the large number of seeds gene and 37 cultivars without the allele of this gene, which, according to the phenotypic evaluation that confirmed it, shows the reliability and accuracy of the marker used to predict and differentiate cultivars for the program breedings are forthcoming. The logistic regression results also confirmed this result. However, a number of other examined samples also had a high number of seeds, which indicates the existence of other genes affecting the number of seeds per panicle in those lines.
Fatemeh Bagherzadeh, Hannaneh Mirahmadi, Soraya Pourtabrizi, Ali Kazemipour, Maryam Dorraninejad, Roohollah Abdoshahi,
Volume 11, Issue 1 (9-2024)
Abstract
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