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Showing 2 results for Zenalinezhad
Saeed Bagherikia, Mohammadhadi Pahlevani, Ahad Yamchi, Khalil Zenalinezhad, Ali Mostafaie,
Volume 4, Issue 1 (9-2017)
Abstract
Under drought stress conditions, as one of the most important limiting factors of grain yield in wheat at arid and semi-arid regions, the remobilization of assimilates gain would be more valuable to grain filling. There are a few reports on the importance of remobilization of the root during the grain filling period under drought stress conditions. An advanced mutant line of bread wheat (T-65-7-1) along with its wild type (cv. Tabasi), were planted at two moisture conditions (normal and 30-40% of field capacity) as a factorial experiment based on a completely randomized design with three replications. Sampling for gene expression analysis was conducted from the root in two stages (7 and 21 days after anthesis). In these genotypes, fructan remobilization, efficiency of fructan remobilization, and relative expression of genes involved in the synthesis and hydrolysis of fructan during the grain filling period, in root, were studied under terminal drought stress. The results showed that the stored fructan in the root participated in the assimilate remobilization. Higher fructan remobilization through root to grain in mutant line under drought stress conditions was due to over-expression of genes involved in the synthesis of fructan (1-SST and 6-SFT) at 7-days after anthesis and in hydrolysis of fructan (6-FEH) at 21-days after anthesis, compared to wild type. Drought stress did not cause a significant change in gene expression of 1-FFT and 1-FEH genes in the root of both genotypes, which confirms the only β (2,6) linkages as predominant form of fructan has affected under drought stress conditions. In wheat breeding programs, 1-SST, 6-SFT and 6-FEH can be used as molecular markers for selecting genotypes with high fructan content and more remobilization.
Arash Salami, Mohammadhadi Pahlevani, Khalil Zenalinezhad, Mohsen Esmaeilzadeh Moghaddam,
Volume 5, Issue 1 (9-2018)
Abstract
Collection and conservation of germplasm, particularly for landraces of important agronomically species and organizing their information, cause to continuity of their usefulness and reduce the risk of their genetic erosion. In this experiment, 10 Iranian wheat landraces along with the Chinese Spring cultivar, as control, were used to assess inter and intra populations variation by using ISSR molecular markers and morphological traits. Evaluation of populations in terms of height, spike length, awn length, number of spike nodes, number of grains per spike, flag leaf length and grain weight showed that there were considerable variation among landraces. Also, in some landraces like KhorramAbad and Ardabil, intra population diversity for some of these traits, such as plant height and length of awn, was obvious. Evaluation of the landraces for ISSR markers showed that among 99 scored bands, 78 bands were polymorphic. Percent of polymorphism with mean of 81.88 % was varied from 53.33 for primer of ISSR-4 to 100% for primers of ISSR-9, ISSR-14, ISSR-5 and ISSR-7. Average PIC of the landraces was estimated 3.0. Results of this study showed that there was considerable intra population diversity for the ISSR markers in landraces of KhorramAbad, Maragheh and Torbat-e-Heydarieh however, a little diversity for Khoy, Ahwaz, Isfahan, Mashad, Urmia, Shiraz and Ardabil was observed. Results of this study showed that there is inter and intra genetic diversity within Iranian landraces of wheat with different levels and these landraces can be used as basic population for extraction of pure lines.
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