|
|
|
 |
Search published articles |
 |
|
Showing 3 results for Shiran
Seyedeh Zahra Hosseini, Behroz Shiran, Mohammad Ali Ebrahimi,
Volume 5, Issue 2 (3-2019)
Abstract
Phylogenetic relations among 12 wild species of almonds, one cultivated almond and one species of peach were investigated by using of ITS1-5.8S rDNA-ITS2 sequences and trnL region of chloroplast DNA. To do this, maximum-parsimony and neighbor joining analysis adopted. Results of ITS data showed that studied species of Prunus only divided in two groups but incapable to separate different sections. P. tenella showed more diverse genetic distance in compare to other studied species and it seems that this species does not belong to Amygdalus. Also, by using the ITS data it can be reported that Prunus is monophyletic. In this research, the genetic distances for each pair of species were determined and the average genetic distance between species shows only the lowest genetic distance within the genus. Therefore, Prunus is a single genus. Regarding its high similarity of trnL region in wild almond species, it can be reported that maternal ancestor of Prunus is the same and trnL is not optimum marker to separate species of almond.
Kobra Arab, Rudabeh Ravash, Behrouz Shiran,
Volume 6, Issue 2 (3-2020)
Abstract
Increasing drought tolerance, especially in rice, which is one of the most important crops in Asia, is necessary. Transcription factors are specific sequence DNA-binding proteins that are capable of activating or suppressing transcription. These proteins regulate gene expression levels by binding to cis regulatory elements in the promoter of target genes to control various biological processes such as growth, cell division and response to environmental stresses. In this study, MAD8 and MYB93 genes that were involved in drought stress in rice were considered in two leaf and anther tissues at 0, 24, 48, 72 h and one week after stopping irrigation. The results of q-PCR analysis showed significant expression changes of these transcription factor genes under drought stress conditions. In this study, a significant increase in the expression of these genes at 24 h after drought stress in transgenic plants (Promoter region with accession: NC_029264.1 and GUS gene have transformed) compared to non-transgenic plants showed a relationship between these transcription factors and higher expression of transported promoter in transgenic plants.
Faraneh Roshan, Mohammad Rabiei, Behrouz Shiran,
Volume 11, Issue 1 (9-2024)
Abstract
Violet plants (Viola sp.) belonging to the Violaceae family are ornamental plants that could be used for drug design due to their cyclotidic compounds. In this study, 21 different ecotypes of violets were collected from the northern regions of Iran. After DNA extraction, the genetic diversity of ecotypes was investigated using the iPBS molecular marker. Twelve iPBS primers used during the present investigation resulted in 214 bands. The average percentage of observed polymorphism, polymorphic information content (PIC), and marker index (MI) were calculated to be 31.92%, 0.35%, and 5.64% respectively. The Nei genetic distance index ranged between 0 and 0.66. The results of this study indicated a considerable genetic diversity among the violet ecotypes and the efficiency of the iPBS marker in detecting polymorphism. The population genetic analysis showed that 61% of the diversity was associated with intra-species diversity. The species V. odorata and V. alba exhibited the greatest degrees of polymorphism, effective allele number, Shannon index value, and heterozygosity ratios. Also, the dendrogram depicted the close genetic relationship between these two species, as evidenced by Nei's genetic distance measurements. In general, considering the existing taxonomic information and the results obtained from this experiment, it can be concluded that the use of the iPBS marker was highly effective in systematic studies of the genus Viola. The results of this experiment led to the appropriate differentiation of ecotypes and species, which could be used in further breeding studies.
|
|
