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Showing 2 results for Sheikh
Hossein Astaraki, Peyman Sharifi, Fatemeh Sheikh, Volume 6, Issue 2 (3-2020)
Abstract
In order to estimation genotypic correlation and heritability of some faba bean traits, 26 faba bean genotypes were evaluated in a randomized complete block design with three replications during 2014-16 growing seasons in Agricultural Research Sation of Borujerd located in Lorestan province, Iran. The restricted maximum likelihood (REML) was used to estimate the genotypic and phenotypic correlations, broad sence heritability and genetic gain. Analysis of variance based on least squares and REML indicated significant effect of genotype on days to maturity, plant height, hundred seed weight, pod length and dry seed yield. Genotype ×year interactions were significant on all of the traits except of pod length. Borujerd cultivar (G26) and G20 had the highest dry seed yield in both of years. REML results indicated a significant positive genetic correlation between dry seed yield and biological yield and plant height. Also, there were a significant negative phenotypic correlation between dry seed yield and days to maturity, and significant positive phenotypic correlation between dry seed yield and plant height, biological yield and harvest index. So, the selection of early maturing genotypes with a higher yield is achievable and selection can be done to improve the performance of dry seed yield. Cluster analysis indicated variability among genotypes. According to values of broad sence heritability and genetic gain for plant height and high genetic correlation of this trait and dry seed yield; plant height can be used as a suitable trait for improving dry seed yield by selection.
Syed Mehran Alavi Mehryan, Nasser Zare, Asad Masumiasl, Parisa Sheikhzadeh, Rasool Asghari, Volume 7, Issue 1 (9-2020)
Abstract
Ferulago angulata (Schlecht) Boiss is one of the valuable and endemic medicinal plants of Iran, which is of great importance due to the source of terpenoid compounds and antimicrobial properties. In current study, the effects of different concentrations of salicylic acid and yeast extract in cell suspension culture of F.angulata on expression pattern of the HMGR and GPPS genes (involved in terpenes biosynthesis) were investigated for the first time. The F. angulata cell suspension cultures were initiated and established using calli derived from leaf explants, and salicylic acid and yeast extract elicitors (with 50, 100 and 150 mg/L concentrations) were added to the cultures during active growth. Then, the cell samples were prepared at 24, 48 and 72 hours after treatment. Analysis of expression pattern of HMGR and GPPS genes using Real-time PCR showed that the expression of both genes were significantly influenced by the type and concentration of the elicitors and also the times after treatment. The relative expression of HMGR and GPPS genes under elicitors were increased compared to the control, and furthermore, the increase in the relative expression of these genes under salicylic acid treatment was significantly higher than that of yeast extract treatment. The highest relative expression of GPPS and HMGR genes was related to 100 mg/L salicylic acid treatment at 24 hours after treatment. However, the highest relative expression of these genes was observed under the 24 and 72 hours after treatment of 150 mg/L yeast extract. The results of this study could be useful in metabolic engineering of F. angulata.
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