Knowledge about the amount of genetic diversity and understanding relationship between species and landraces is an important step in plant germplasm conservation. In this study, within and between genetic diversity of 17 dill landraces (Anethum graveolens L.) from different areas of Iran was evaluated using five ISSR markers. In total, 29 polymorphic bands were generated. The average of polymorphism was 54.7%. The highest and the lowest values of Polymorphic Information Contents were 0.46 for ((CA)₈G primer) and 0.40 for ((AG)₈T primer), respectively, and with an average of 0.43. Based on the highest and the lowest indices of Polymorphic Loci (0.392 and 0.248), expected heterozygosity (93.10 and 62.07) and shannon's Information Index (0.567 and 0.360) between all populations, the highest and lowest genetic diversity was detected among Ardebil and Azarshahr genotypes, respectively. The genetic dissimilarity matrix showed that Sari and Kerman populations had the highest genetic distance and Ardabil and Borazjan populations had the lowest ones. Partitioning variations within and between populations, using an analysis of molecular variance (AMOVA), showed that 12% of the total genetic variation existed between growing regions. Cluster analysis based on UPGMA method showed a poor relationship between genetic distance and the geographical grouping of dills.