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Showing 1 results for Nejadsadeghi
Seyede Maryam Seyed Seyed Hassan Pour, Leila Nejadsadeghi, Zahra Sadat Shobbar, Danial Kahrizi,
Volume 10, Issue 2 (2-2024)
Abstract
Camelina )Camelina sativa (is an annual, self-pollinating, allohexaploid plant with diploid inheritance belonging to the Brassicaceae family. Camelina exhibits a remarkable degree of similarity to the model plant Arabidopsis thaliana. WRKY transcription factors are among important gene families in plants that play crucial roles in regulating growth and development and in response to diverse stresses. In this research, using bioinformatics analysis and databases, members of the WRKY gene family were identified and their various characteristics were investigated. Overall, the genome of the Camelina plant was found to harbor 214 members of the WRKY gene family. All 214 WRKY genes were found to possess the conserved WRKY functional domain, along with a variety of motifs within their structural composition. Phylogenetic analysis divided the identified members of Camelina WRKY genes into four main groups. Examination of the chromosomal positions revealed that the 214 identified WRKY genes exhibited an uneven distribution across the chromosomes. In order to validate the identified genes, the expression of two genes (Csa11g065620 and Csa07g035970) orthologs of two genes involved in drought stress in Arabidopsis (WRKY8 and WRKY57), were investigated in a drought tolerant (DH 91) and a drought sensitive (DH 101) lines. The results of the gene expression analysis showed that both genes had high expression in drought stress conditions in tolerant line in comparison to normal conditions, whereas no significant expression was found in drought sensitive line. The findings of the present study offer valuable insights for evolutionary investigations and enhance our understanding of the functional roles of the WRKY gene family in Camelina, thereby laying a foundation for future research endeavors in this field.
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