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Showing 5 results for Navabpour
Mohammad Reza Jafarzadeh Razmi, Saeid Navabpour, Hossein Sabouri, Seyedeh Sanaz Ramezanpour,
Volume 6, Issue 2 (3-2020)
Abstract
In order to analyze the genetic components of agronomic traits among 116 F9 recombinant lines derived from crosses of Ahlamitarom × Sepidroud rice cultivars, an experiment was conducted as a randomized complete block design in research farm of Gonbad Kavous University of Agriculture with three replications in 2016 and 2017. Genetic linkage map provided with 80 SSR markers, 28 iPBS Markers (79 polymorphic alleles), 7 IRAP markers (17 polymorphic alleles) and 26 ISSR markers (70 polymorphic alleles), which covered 1275.4 cM of the rice genome. QTL analysis was performed by Composite Interval Mapping. In two years, 15 QTLs detected for the studied traits. The additive effected varied from 6.725 g for grain weight up to -85.626 g for grain weight. Also, R2 for the detected QTLs explained from 11.3% to 20% of the total variation. The highest R2 was related to grain weight in the first year of experiment. Among the detected QTLs, qGWs on chromosome 1, were found to be stable and large effector QTLs for rice (Oryza sativa L.) grain weight, and can be used in marker-assisted breeding and selection programs after validation.
Mahnaz Katouzi, Saeid Navabpour, Hossein Sabouri , Ali Akbar Ebadi,
Volume 7, Issue 2 (3-2021)
Abstract
In order to identify QTLs controlling agronomically traits, landrace Tarom and rice Tarom mutant were crossed. SSR, ISSR, iPBS and IRAP markers were amplified in 250 F2 individuals to prepare the linkage map. Number of tillers, 100 grain weight, number of filled grains, number of unfilled grains, plant height, panicle length, number of branches, stem diameter, grain length, grain width, grain shape, straw weight, days to maturity, flag leaf length and flag leaf width were measured for 250 individuals. The linkage map covered 970.9 cM of rice genome. The distance between two adjacent markers was calculated to be 12.77 cM. Based on the results, a total of 13 QTLs were identified for the evaluated traits. For all studied traits, alleles transferred from the parents to the QTLs detected increased grain yield. Most QTLs were detected for days to flowering. Three QTLs were located on chromosomes 10 and 4 (two QTLs) for days to flowering. qLDF-4a and qLDF-4b had a negative additive effect and the parent alleles of the mutant landrace Tarom reduced the number of days to flowering. These QTLs explained 11.6% of the phenotypic variance. Since the population under study was derived from a cross between landrace and mutant Tarom cultivars and the resulting population varied only in the mutated genes; so, the QTLs detected in this study were more accurate in location and expression levels, and after validation of them, they could be recommended for marker assistant selection breeding programs.
Saeid Navabpour, Ahad Yamchi, Sasan Golcheshmeh,
Volume 8, Issue 1 (8-2021)
Abstract
The present study was performed to classify and study genetic diversity between Calotropis procera accessions from different regions of Kerman province (Iran) using ISSR markers. In total, DNA from 14 plant samples with nine ISSR primers was amplified by PCR and their banding pattern was obtained. The primers showed acceptable polymorphism (35.93) and minimum and maximum polymorphic information content (PIC) of primers in this study were 0.11 for ISSR9 primer and 0.41 for ISSR3 and ISSR8 primers, respectively. Genetic similarity based on Nei index was varied from 0.405 to 0.745 and the lowest genetic similarity was found between J3 (Related to Jiroft) and D2 (Related to Dosari) and the highest genetic similarity was found between J1 and J2 (both of them for Jiroft). By using UPGMA cluster analysis, samples divided into four groups, and the second and third groups contained more accessions. In terms of genetic similarity, two accessions of Jiroft 1 (J1) and Jiroft 2 (J2) which classified in the same cluster were closer. Also, the accessions collected from Anbarabad were at a longer genetic distance than other accessions. Principal coordinate analysis also showed that the first and second components justify 67 percent of obtained genetic diversity. In general, ISSR markers were useful for classifying Calotropis procera accessions and according to the obtained information about existence of genetic diversity between Calotropis procera accessions of Kerman province, this diversity could be useful in the future for breeding and production of Calotropis procera.
Saeid Navabpour, Horeyeh Najafi,
Volume 9, Issue 2 (3-2023)
Abstract
Environmental stress is one of the main factors that reduce the growth and performance of crops and threatening human food security. This study was conducted in order to investigate the effect of drought stress on the changes in biochemical traits and the level of expression of a MYB transcription factor gene in two wheat cultivars (Tajan and Zagros), under drought stress. The experiment was conducted as a factorial based on a completely randomized design with 3 replications. Drought treatments were applied at three levels of 40, 70 and 100% of field capacity 4 weeks after germination. Twenty days after the application of stress, leaves and roots were sampled in order to investigate the expression of MYB genes and measuring some biochemical traits. The results of examining the chlorophyll content under stress showed that the content of chlorophyll a and b decreased with increasing of stress intensity in different genotypes. The rate of reduction of chlorophyll a and b in Tajan genotype under severe stress was higher than Zagros genotype. Also, TBARM content under severe drought stress was significantly higher than moderate stress condition and this increase was seen in Tajen genotype more than Zagros genotype. qRT-PCR analysis showed that the MYB genes showed an increase in expression under drought stress. Furthermore, Zagros genotype, which is considered as a tolerant cultivar to drought stress, had a higher MYB expression level than Tajan cultivar for both genes, suggesting this cultivar for future breeding programs, also considering the importance MYB family genes during drought stress, the results can be used in molecular breeding and pyramiding breeding projects.
Seyedeh Sanaz Ramezanpour, Hassan Soltanloo, Saied Navabpour,
Volume 10, Issue 2 (2-2024)
Abstract
To evaluate the effect of fungus Blumeria graminis (powdery mildew disease) on expression of genes associated with resistance reactions in barley, a susceptible cultivar (Afzal), a semi-susceptible genotype (Line 67) and a resistant genotype (Line 104) were selected. Following inoculation with Blumeria graminis at seedling stage, sampling was performed at different time points (0-10 days). Changes in gene expression levels were measured by qRT-PCR analysis. Analysis of molecular data showed that the genes encoding chitinase and glucanase as the key enzymes in fungal cell wall degradation, had higher expression levels in the resistant genotype (Line 104). The transcript level of chitinase in semi-susceptible genotype (Line 67) was lower than that of the resistant genotype (Line 104) and higher than that of the susceptible cultivar. Most transcripts of chitinase gene were seen at 12 hours post inoculation in the resistant genotype (Line 104), whereas the lowest expression level was recorded at the same time in the susceptible cultivar. The expression levels of the other two genes (glucanase and peroxidase) were higher in the resistant genotype (Line 104) than those in the susceptible cultivar. Increasing in MAPK transcripts in resistant genotype (Line 104) and its depletion in susceptible cultivar confirmed MAPK role in Hypersensitive response (HR) and defense responses of barley infected with powdery mildew disease. Based on the findings of this study, it appears that the HR in the resistant genotype initiated as early as six hours post inoculation, effectively hindering the penetration and dissemination of the pathogen within the plant. Such reaction was not observed in the semi-susceptible and susceptible barley plants, possibly due to delayed in responses, allowing the pathogen ample time to penetrate and propagate within the host plant. The results of this research can be used to evaluate the resistance level of cultivars and also to evaluate the resistance in the seedling stage of promising lines.
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