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The production of human gamma interferon in eukaryote expression systems refers as a therapeutic recombinant protein which has significant impact in medical studies. Unique com position of gamma interferon makes such protein as a suitable tool against cancer. It is documented that phosphinothricin (PPT) classified as non-selective herbicide group of bialaphos acts an inhibitor for glutamine synthetase. The bar gene is encoding the phosphinothricin-N-acetyltransferase (PAT) enzyme. This enzyme capable of boosting resistance against PPT herbicide, thus it can be selected as a selective marker within plant population. Then various colony PCR techniques, enzymatic digestion and sequencing were used to confirm the accuracy of fusion of IFNγ-bar gens within expression transporter. Using freezing and thawing method to transfer the pCAMBIA1305.1- IFNγ-bar construction into strain of LBA4404 of agrobacterium, then disc leaves was used to integrate into the genomic of tobacco plant. The transgenic plants were selected under selector condition which possess 30 mg/l of hygromycin. After the developed roots were transferred into soil, and PCR technique was used to confirm the presence of IFNγ-bar in the genomic of plants. Dot blot analysis was applied to detect IFNγ-bar protein in transgenic of to tobacco plants.

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Yarrow (Achillea millefolium) is an herbaceous and perennial plant species which belongs to the Asteraceae family. Yarrow's essential oil has different compounds of monoterpene and sesquiterpens, which its main constituents are pinene and linalool. These compunds have anti-microbial and anti-pest activities and also can be used in the food industry, perfumery and cosmetics.The aim of the present study was to use the degenerate primers approach in order to isolate Pinene synthase and linalool synthase genes from Yarrow plant. Up to date, there is no any report on the availability of these genes in the world gene bank. In this investigation the total RNA was extracted from Yarrow then pinene synthase and linalool synthase genes were isolated, using degenerate primers and Polymerase Chain Reaction (PCR). PCR amplified two bands of 250 bp and 720 bp. The sequence data were compared with NCBI gene bank data. The results of diversity study among varieties and families based on Pis and Lis genes showed most similarity between Achillea and Artemisia plants. Also this similarity was seen between Asteraceae and Lamiaceae families and these families grouped together same group. These results also showed a relatively high similarity of Pis and Lis with some other plants which confirmed sequencing data.