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Showing 6 results for Mousav
Elham Ghazizadeh, Amir Mousavi, Faranak Hadi,
Volume 1, Issue 2 (2-2015)
Abstract
As genetically modified organisms (GMOs) development is now increasing, detection and determination of their quantitative threshold using reliable methods would be necessary. The goal of this study was to introduce a sensitive method for qualitative and quantitative detection of Roundup-ready soybean samples. For primary screening, semi-quantitative molecular assays have been used for detection of various percentages of transgenic and non-transgenic Roundup-ready soybean samples. Furthermore, an experiment was conducted using the CaMV 35S primers in combinations with soybean lectin-specific primers in two imported samples of soybean seeds. Real-time PCR-based analysis indicated that the amount of GMO material in the seeds and the limit of detection (LOD) obtained for 35S sequence was less than 1%. The sensitivity and accuracy of this method had conformity with the international standards of seed labeling. This is the first report of its type for quantitative detection of a genetically modified material in a commercial seed lot in Iran.
Hadi Karimbeigi, Farhad Nazarian-Firouzabadi, Mitra Khademi, Elham Mousav,
Volume 3, Issue 1 (9-2016)
Abstract
Oilseed rape (Brassica nupus L), a member of Brassicaceae family, is an important crop regarding oil production worldwide. Brassicaceae is an economically important family of flowering plants with about 350 genera and more than 3000 species. Eleven pairs of single sequence repeat (SSR) primers were used to identify the genetic diversity among 21 oilseed rape genotypes. Results of SSR molecular marker analysis revealed that SSR primers produced a total number of 76 scorable bands of which 46 (60.5%) bands were polymorphics. The average number of bands for each primer and genotype was 6.9 and 3.6, respectively. Both CB10036B and Na10A09 primers produced 10 and Cb10403 primer produced 4 polymorphic bands, respectively. UPGMA cluster analysis based on Dice similarity matrix showed that Zarfam and Gerinimo genotypes had the highest (0.99%) and Licord and KS-11 genotypes had the lowest (0.72%) similarity. Both Iranian and foreign genotypes were grouped together in one major cluster, indicating presumably they may have the same origin and/or common pedigree. Results of AMOVA analysis within and between groups (spring – Autumn) revealed that almost 97% of total genetic diversity belonged to within group genotypes.
Reza Darvishzadeh, Mir Javad Mousavi Andazghi, Amir Fayyaz Moghaddam, Hossein Abbassi Holasou, Seyyed Reza Alavi,
Volume 3, Issue 2 (3-2017)
Abstract
In order to evaluate heritability and gene action for some of the important quantitative traits in oriental tobacco, two genotypes, Basma 16-10 and SPT406 were crossed with Basma S. 31 cultivar, separately in 2009-2010. Parents with F1, F2, BC1 and BC2 generations were planted in a randomized complete block design with three replications. Traits such as plant height, leaf length, leaf width, leaf number, internnode number, stem diameter and yield per plant were recorded. The results obtained from analysis of variance indicated that generations mean squares were statistically significant for all traits expect for stem diameter. Therefore, generation mean analysis was performed for significant triats to estimate gene actions using Chi-square and scaling tests. The Chi-square of simple three-parametric model (additive-dominance model) was significant for studied crosses, indicating the presence of non allelic-interactions in the inheritance of these traits in oriental tobacco. Both additive and dominance genetic effects were significant for plant height, leaf length, leaf width, leaf number and internnode number. In addition, presence of high amount of dominance effect and dominance × dominance interactions suggests the importance of non-additive genetic effects for these traits in oriental tobacco. Therefore, selection for these traits in early generations can not be successful. However, additive genetic effects play an important role in the inheritance of yield, and then selection for this trait is hopeful in early generations during tobacco breeding process.
Seyed Sajad Sohrabi, Seyyed Mohsen Sohrabi, Seyed Karim Mousavi, Mohsen Mohammadi,
Volume 7, Issue 1 (9-2020)
Abstract
Saffron (Crocus sativus L.) is the most valuable and expensive spice in the world. The stigmas of saffron are the source of valuable apocarotenoids such as crocin, picrocrocin and safranal. transcriptomic and expression studies of genes are important steps in investigating of secondary metabolites in plants. One of the important prerequisites for such studies is the existence of reliable and stable reference genes to normalize the expression of other genes. In the present study, eight reference genes were identified and isolated using transcriptome of saffron and their expression stability was evaluated by nonparametric statistics and methods. The results of amplification and sequencing showed accurate identification of eight reference genes Actin, EF1, GAPDH, H3, MDH, TBP, UBC and UBQ. The expression stability evaluation revealed that MDH and UBQ genes had the highest stability among different saffron tissues and TBP had the lowest stability among them. In this study, for first time, eight reference genes were isolated from saffron and their expression stability was evaluated. The reference genes identified in the present study can be used as stable genes to normalize gene expression in transcriptomic and expression studies of saffron plant.
Seyyed Mohsen Sohrabi, Seyed Karim Mousavi,
Volume 9, Issue 2 (3-2023)
Abstract
Chickpea (Cicer arietinum L.) is one of the most important crops in the world. After bean and pea, chickpea is the most important cold season legume. Weeds are one of the most important threats to chickpea production worldwide. Due to the sensitivity of chickpea to herbicides, the majority of herbicides are used pre-emergence and the use of post-emergence herbicides is limited, and therefore weeds cause a significant decrease in chickpea yield. Therefore, herbicide-tolerant chickpea cultivars that have a higher flexibility for post-emergence herbicide application are needed to improve the chickpea yield. In this study, using seed bioassay and PCR method, resistance mechanism of Iranian chickpea cultivars to Pursuit herbicide was investigated. The results showed a significant genotypic and phenotypic variation among Iranian chickpea cultivars for tolerance to the Pursuit herbicide. The results did not show a difference between the target genes of Pursuit herbicide, ALS1 and ALS2, in all investigated cultivars with that of the reference sequences in the GenBank. This proves that the resistance observed in different chickpea cultivars to the herbicide Pursuit is not associated with the target site resistance mechanism and probably follows a non-target resistance mechanism. The superior genotypes of this study (Bivanij, Aksou, Mansour, TDS-Maragheh90-400 and TDS-Maragheh90-358) can be recommended to farmers and also suggested as parents to produce natural herbicide resistant chickpea plants in breeding programs.
Seyedeh Somayeh Mousavi, Omidali Akbarpour, Dr Tahmasb Hosseinpour,
Volume 10, Issue 1 (9-2023)
Abstract
In this research, 15 bread wheat genotypes along with Aftab variety as a control variety were implemented with 4 replications in the form of randomized complete block design for 3 crop years (2016-2019) at Sarab Chengai Station in Khorramabad. The likelihood ratio test (LRT) showed that the genotype-year interaction effect was significant for grain yield. Based on this, singular value analysis (SVD) was performed on the matrix of best linear unbiased predictions (BLUP) of genotype × year interaction to evaluate the stability of genotypes. The scree plot showed that the first principal component accounted for 71.7% and the second principal component accounted for 28.3% of the matrix changes resulting from the best unbiased predictions of the genotype interaction per year. The biplot of the first principal component of the environment against the nominal yield also showed that genotypes No. 9, 12 and 13 had a negligible contribution to the genotype × year interaction and had higher general stability. Also, the biplot of grain yield against the weighted average of absolute scores (WAASB) placed the genotypes in four regions, so that genotypes No. 15, 16, 12, 11, and 10 are in the fourth region due to high stability (low values WAASB) and magnitude of response variable (high performance) were identified as superior genotypes. The WAASBY index (weighted average of WAASB stability and performance) identified genotypes No. 15, 16, 12, 10, 11, 14, 9 and 4 as stable and high yielding genotypes. In general, based on WAASB and WAASBY indices and their comparison, genotypes 15, 16, 12, 11 and 10 were selected as the best genotypes that can be recommended for cultivation in similar climates.
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