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Showing 2 results for Majdi
Mohammad Majdi, Ghasem Karimzade, Mohammad Ali Malboobi,
Volume 1, Issue 2 (2-2015)
Abstract
Feverfew (Tanacetum partheniumL. Schulz Bip.) is a medicinal herb belonging to the Asteraceae which recently raised researcher’s attention due to its medicinal value and pharmacological activities, especially as a migraine prophylaxis agent and also for treatment of cancer. Parthenolide has a sesquiterpene lactone structure which is most likely synthesized through the mevalonic acid (MVA) pathway. Recently, it has been shown that there is cross a talk between the MVA and the MEP pathways through IDP (Iosopentenyl diphosphate) exchange as a precursor for the biosynthesis of different terpenes hence, parthenolide biosynthesis could be affected by the MEP pathway as well. In the present work, the relative gene expression of two key genes of the MVA pathway, including 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) and germacrene A synthase (GAS) and two key genes of the MEP pathway including 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) and hydroxy-2-methyl-2-(E)-butenyl4-diphosphate reductase (HDR) were examined in leaves derived from the vegetative or generative phases, also in flowers using real time PCR. The results of our study showed that expression of these genes depend on the growth stage and genotype. Moreover, in which in the flowers and leaves derived from vegetative phase in different genotypes only the TpGAS gene expression showed a significant difference, while in the leaves derived of generative phase the relative gene expression showed a significant difference for TpHMGR, TpGAS and TpDXR.
Rizan Elyasi, Mohammad Majdi, Abdolbaset Azizi,
Volume 8, Issue 2 (3-2022)
Abstract
Black cumin (Nigella sativa) is a medicinal plant of the Ranunculacea family which raised attention due to its pharmaceutical properties. Medical significance of N. sativa mainly attributed to its oxygenated monoterpenes which are biosynthesized via the methyl erythritol phosphate (MEP) pathway located in plastids. In this study, the essential oil components of leaves, flowers, and developmental stages of seed including half black seeds, soft black seeds, and hard black seeds were analyzed in N. sativa. Whereas no terpene was detected in flowers and leaves, seeds were found to be the major site of biosynthesis and accumulation of terpenes, and the amount of terpene compounds changed during seed maturation. The essential oil consists of monoterpenes (more than 99%) and sesquiterpenes (less than 1%). In order to improve our understanding of monoterpene metabolism, the partial sequence of a hypothetical monoterpene synthase (NsTPS2) was isolated from N. sativa plant using RACE-PCR technique. This monoterpene synthase was identified from RNA sequencing data from soft black seeds. Except of the highly conserved DDXXD motif in NsTPS2 which is necessary to validate monoterpene synthases, no other conserved regions of other identified monoterpene synthases were observed. Dendrogram analysis revealed that NsTPS2 had the highest homology with a terpene synthase (72.89%) from Aconitum carmichaelii and these two sequences were grouped in the same group. Nigella sativa and Aconitum carmichaelii both belong to the Ranunculacea family. This indicates that the genetic information of plants of the Ranunculacea family can be used to isolate different monoterpene synthase. The results of this research can be useful in genetic manipulation and metabolic engineering of Nigella sativa.
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