In this study, genetic diversity of 25 rainfed bread and durum wheat genotypes were assessed using 20 SSR primers that all of them were generated scorable bands. Totally 69 alleles (ranged between 2 allele for Xcfd40 and Xgwm369, and 5 allele for Xbarc54 primers per each locus), were distinguished. Polymorphic information content (PIC) for all SSR primers was calculated. The highest (0.98) and the lowest (0.64) amount of PIC was pertained to Xcfd40 and Xgwm30 primers, respectively. Based on similarity matrix, the highest and lowest genetic similarity was belonged to Seri82 and Seri (0.86) and Sita/chil and Baviacora (0.14), respectively. Cluster analysis could distinct spring and winter wheat genotypes and as well as bread and durum wheat genotypes. It was concluded that SSR marker was suitable for evaluation of genetic diversity in rainfed wheat genotypes. This genetic diversity can be used in wheat breeding programs.