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Showing 3 results for Javadi
Raheleh Aziznia, Hedieh Badakhshan, Taimoor Javadi, Soma Zamani,
Volume 6, Issue 2 (3-2020)
Abstract
In this study, variation of beta-glucan content was assessed in 20 barley line and cultivars based on complete block design with three replications. Genetic diversity of these genotypes was also evaluated using ISSR markers. Beta-glucan extracted by an enzymatic method. Significant differences were found at the level of 1% among barley genotypes for beta-glucan content. The beta glucan content was variable from 7.21 to 12.48 and, the Yosef, E94B3 and E94B17 genotypes hold the highest content of the beta-glucan. ISSR primers with average polymorphism of 66.79%, genetic diversity of 0.25 and Shannon index of 0.37 were determined as efficient markers for studying genetic diversity. The barley lines and cultivars were assigned in two distinct groups according to their genetic pedigree. On the basis of non-parametric Kruskal-Wallis, Spearman correlation, and stepwise regression analysis, nine informative primers were detected explaining highest seed’s beta-glucan content variations ranging from 24.3 to 42.4 percent. The ISSR6 (700), the combination of ISSR1+ISSR4 (1400) and IS2+ ISSR2 (1400) primers were the most informative primers for the beta-glucan content. The informative markers provide possible functional and efficient marker based selection method and, screening the barley germplasms for the highest beta-glucan content.
Zahra Gholamzadeh, Hamideh Javadi, Maryam Pezhmanmehr, Mehrnaz Hatami,
Volume 7, Issue 1 (9-2020)
Abstract
Astragalus with more than 800 species is one of the largest plant species in Iran and most of its species are medicinal and native. In this study, karyotypic characteristics of 21 populations from 13 species including A. vegetus, A. iranicus, A. vanillae, A. chrysostachys, A. glycyphyllos, A. bombycinos, A. campylorrhynchus, A. macrourus, A. caragana, A. ebenoides, A. pseudocyclophyllus, A. stevenianus and A. jodostachys were examined and compared. The results showed that there was a significant difference between the populations in terms of all measured traits (TL, LA, SA, AR, CI, TF%, DRL, LA% and SA%), except for traits A1 and A2. Population 25786 of A. vegetus species from Khorasan with average length of 4.51 μm had the highest total chromosome length and population 13764 of A. vanillae species from Qom with 2.67 μm had the lowest mean total chromosome length. According to the values of AR, CI, TF%, A1, A2, DRL, LA% and SA%, population 3421 of A. vegetus species is the most asymmetric and population 29586 of species A. campylorrhynchus had the most symmetrical karyotype.
Hamideh Javadi, Parvin Salehi Shanjani,
Volume 10, Issue 2 (2-2024)
Abstract
German chamomile, Matricaria recutita L., is a medicinal plant of Asteraceae family, which is known as the "star" among medicinal species and its use dates back to the time of the Egyptian pharaohs. In this study, four populations of M. recutita that were collected from different parts of Iran, were studied for chromosome and karyotypic characteristics. Squash method with Hematoxylin staining was used to prepare microscopic slides. Microscopic observations showed the number of somatic chromosomes was 2n=18 for all populations. The length (TL, LA and SA) and symmetry karyotype (AR, CI, TF%, DRL, SC, LA% and SA%), as well inter and intra chromosome asymmetry (A1 and A2) were calculated. Karyotypic formula analysis showed the predominance of metacentric chromosomes in all populations. Based on mean of measured traits, the longest chromosome and genome belonged to population 15123 (Arak). According to Stebbins classification, populations were categorized in two groups. Karyotype symmetry factors showed that two populations 15123 (Arak) and 8959 (Borujen) had asymmetric and symmetric karyotypes, respectively. In PCA, the first three components could explain the entire diversity of populations. The most diversity of populations was for chromosome length traits. According to Ward's clustering method, the populations were placed in three groups at a genetic distance of 2.32. In general, the existence of high genetic and cytogenetic diversity indicates the existence of high potential in the improvement of Matricaria genus and cross-breeding activities. The data obtained from this research are used in the future breeding programs of Matricaria L.
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