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Showing 2 results for Jafari
Mahdieh Sahshorpour, Jafar Amani, Mahyat Jafari, Ali Hatef Salmanian,
Volume 1, Issue 1 (5-2014)
Abstract
One of the important pathogens which cause hemorrhagic colitis and hemolytic uremic syndrome in humans is enterohemorrhagic Escherichia coli (EHEC) O157:H7. Cattle are the most important reservoir of this bacterium. EspA and Intimin are two protein factors for bacteria colonization on intestinal epithelium and cause attaching/effacing lesion. The LEE pathogenicity islands code these proteins. EspA is part of type III secretion systems which delivers Tir to the host cell and integrate to membrane. Intimin encoded by eae gene and fused to Tir. In this research we supposed that chimeric recombinant form of EI gene containing EspA and Intimin were fused with a linker as an edible candidate vaccine would reduce colonization of E. coli O157:H7 in animal model. We constructed a synthetic gene EspA (E120) and intimin (Int282) fused by (EAAAK)4 sequence. The synthetic gene (EI) was codon optimized and subcloned into plant expression vector (PBI121) under CaMV35S promoter and then transferred to tobacco plant by agrobacterium mediated protocol. The presence of inserted gene in plant genome was documented by PCR and RT-PCR methods. The amount of EI protein in transgenic tobacco leaves were estimated 0.1% of the total soluble protein (TSP) by ELISA method.
Soheila Afkar, Faranak Hadi, Ali Ashraf Jafari,
Volume 8, Issue 2 (3-2022)
Abstract
Festuca is one of the largest genera of the grass family, which has more than 600 species with different ploidy levels. The aim of this study was to estimate the genetic diversity within 22 populations of three species of Festuca (Festuca arundinacea, F.rubra and F.ovina) using a seed storage protein electrophoresis pattern. These species showed a significant variation in the number of protein bands from 5-13. The highest number of bands was found in G17 (F.rubra) and the lowest number of protein bands was in G5 (F.ovina). Band number 14 was only observed in G3. It is suggested that this band can be considered as a specific band for the identification of this genotype. According to the results of AMOVA analysis, there is a high level of genetic diversity within the species rather than between species that can be due to the out-crossing nature of this genus. According to observed differences for variation parameters among the three studied species, it is concluded that they have dissimilar genetic structures. The results of cluster analysis based on seed storage protein profiles in evaluated genotypes using Euclidean distance matrix and UPGMA method showed four groups. The lowest similarity coefficient was between G14 and G15 (F.arundinacea) with G6 (F.ovina). Hence, it is suggested that they evolved from a different evolutionary process and it is suggested to use them as the parents of new synthetic varieties. The observed diversity in the seed protein pattern in the three species of Festuca, can be explained by allogamy-induced-heterozygosity, species difference or population collection from various regions.
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