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Showing 4 results for Hatami
Roya Zirak, Ali Soleimani, Mehrshad Zeinolabedini, Hamid Hatami Maleki, Azizolah Kheiri,
Volume 5, Issue 2 (3-2019)
Abstract
Genetic diversity among Russian olive genotypes in three different regions of East-Azerbaijan province (includes Tabriz, Maragheh and Malekan) were assessed using morphological and molecular (AFLP) markers. Results of the quantitative and qualitative traits statistics showed a significant genetic variation among studied germplasm and categorized them in five distinguished groups. The most number of genotypes from Maragheh region were in group 1, while, the group 3 consisted of the majority of genotypes from Tabriz region. All genotypes were analyzed with 14 EcoRI-MseI primer combinations. A total of 439 informative and polymorph AFLP markers was generated and analyzed. Based on Jaccard Similarity Index, the minimum genetic similarity was observed between genotype 19 (from Malekan region) and 27 (from Maragheh). The principal coordinate analysis (PCoA) showed the suitable genomic distribution of AFLP markers among individuals. The highest (0.83 %) and lowest (0.33 %) polymorphic information content achieved by primers combination MTTT-EGA and MGT-ETA, respectively. Cluster analysis using molecular data and UPGMA algorithm, classified the studied genotypes in three distinguished groups. The genotypes at the same geographical region did not classify in the same group based on clustering by molecular data. However, this achieved to a large extent when they were classified groups using morphological traits. This result might be explained in terms of convergent evolution and some in part due to asexual propagation of Russian olive from the limited maternal genotypes and distribution across the different geographical regions.
Somayeh Dadashi, Reza Darvishzadeh, Mojtaba Nouraein, Hamid Hatami Maleki,
Volume 6, Issue 1 (9-2019)
Abstract
For the purpose of graphical analysis and estimation of genetic parameters related to yield and its components, six tobacco genotypes was crossed in half -diallel mating design. In this study, the F2 progenies and their parents were evaluated in a randomized complete block design with three replications. The results of analysis of variance indicated a significant difference between genotypes for plant height, leaf number, leaf dry weight and leaf fresh weight. Hayman graphical method was utilized for analysis of data. Diallel analysis indicated existence of an additive and dominant actions in inheritance of all studied traits. Traits including plant height, leaf number, leaf length and width, stem diameter, internode distance and leaf dry weight possessed greater additive effects meanwhile in the leaf weight the dominance effect was greater. The additive gene action detected for leaf dry weight (yield) manifested the influence of selection methods in breeding of this trait. Due to the fact that the fresh weight of the leaf was controlled by dominance effects, so hybrid-based methods are effective in modifying this trait. Also, additive and dominance gene action were contributed in heritability of yield, plant height and number of leaves. Regarding the mean values of dominance degree and results of graphical analysis, the gene action for leaf fresh weight was over-domnance and so, the heterosis phenomenon could be used to increase and improve this trait. For the traits including plant height, leaf number and yield, the gene action type was relative dominance.
Zahra Gholamzadeh, Hamideh Javadi, Maryam Pezhmanmehr, Mehrnaz Hatami,
Volume 7, Issue 1 (9-2020)
Abstract
Astragalus with more than 800 species is one of the largest plant species in Iran and most of its species are medicinal and native. In this study, karyotypic characteristics of 21 populations from 13 species including A. vegetus, A. iranicus, A. vanillae, A. chrysostachys, A. glycyphyllos, A. bombycinos, A. campylorrhynchus, A. macrourus, A. caragana, A. ebenoides, A. pseudocyclophyllus, A. stevenianus and A. jodostachys were examined and compared. The results showed that there was a significant difference between the populations in terms of all measured traits (TL, LA, SA, AR, CI, TF%, DRL, LA% and SA%), except for traits A1 and A2. Population 25786 of A. vegetus species from Khorasan with average length of 4.51 μm had the highest total chromosome length and population 13764 of A. vanillae species from Qom with 2.67 μm had the lowest mean total chromosome length. According to the values of AR, CI, TF%, A1, A2, DRL, LA% and SA%, population 3421 of A. vegetus species is the most asymmetric and population 29586 of species A. campylorrhynchus had the most symmetrical karyotype.
Fatemeh Hatami, Farhad Nazarian-Firouzabadi, Seyed Sajad Sohrabi, Mitra Khademi,
Volume 11, Issue 1 (9-2024)
Abstract
Saffron (Crocus sativus L.) is not only one of the most expensive food products in Iran and the world but also holds a special place among Iran's export and industrial products. Since saffron is propagated only through corms, preventing the contamination of corms as “seed” with devastating pathogens is crucial to maintaining the quality and yield of the saffron. Hence, investigating the genetic mechanisms associated with the response of the saffron plant to fungal pathogens such as fusarium wilt rot (Fusarium oxysporum) is of great importance. Given that plants express a wide range of resistance genes to defend themselves, the role of genes related to the PTI (Pattern-Triggered Immunity) pathway, such as those in the LysM-RLK family, is crucial in pathogen resistance. Therefore, studying the transcriptome of saffron corms infected with the pathogen Fusarium oxysporum is important for identifying and investigating the genes belonging to the RLP and RLK gene families. Plants express a wide range of resistance genes to respond to pathogens attacks. Among different gene families associated with PTI pathway, the LysM-RLK family plays an important role in resistance to pathogens. Therefore, in this study, the transcriptome of saffron corms infected with the Fusarium oxysporum was studied to identify and investigate the genes belonging to the RLP and RLK gene families. According to the results of this study, a total of 45 genes encoding PTI pathway receptors were identified in the saffron transcriptome, with 40 sequences belonging to the RLP (Receptor-like proteins) family and 5 sequences to the RLK (Receptor-like kinases) family. The expression analysis of some main RLP and RLK family members showed that the highest expression was related to the sequences of Contig 41583 (RLP) and Contig 61879 (RLK) in the saffron stigma and corms, respectively. Furthermore, the expression of the selected genes in the infected corms significantly increased compared to the control healthy corm. Additionally, the expression levels of the target genes (Contig 41583 and Contig 61879) assessed using qRT-PCR indicated higher expression in corms 72 hours post-infection compared to 48 hours post-inoculation. These results suggest that RLK proteins play a crucial role in the interaction between saffron and the pathogen, particularly due to the presence of the LysM motif. Since LysM motif binds to chitin oligomers of fungal cell walls of certain fungi and oomycetes, it triggers plant immune responses. Overall, the findings of this study are significant for understanding the specific nature of the plant-pathogen relationship and can contribute to insights into the PTI immune pathway.
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