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Showing 14 results for Ahmadi
Bahareh Zakerghoran, Hamid Rajabi Memari, Daryoosh Nabati Ahmadi, Marzieh Siahmard,
Volume 1, Issue 1 (5-2014)
Abstract
The production of human gamma interferon in eukaryote expression systems refers as a therapeutic recombinant protein which has significant impact in medical studies. Unique com position of gamma interferon makes such protein as a suitable tool against cancer. It is documented that phosphinothricin (PPT) classified as non-selective herbicide group of bialaphos acts an inhibitor for glutamine synthetase. The bar gene is encoding the phosphinothricin-N-acetyltransferase (PAT) enzyme. This enzyme capable of boosting resistance against PPT herbicide, thus it can be selected as a selective marker within plant population. Then various colony PCR techniques, enzymatic digestion and sequencing were used to confirm the accuracy of fusion of IFNγ-bar gens within expression transporter. Using freezing and thawing method to transfer the pCAMBIA1305.1- IFNγ-bar construction into strain of LBA4404 of agrobacterium, then disc leaves was used to integrate into the genomic of tobacco plant. The transgenic plants were selected under selector condition which possess 30 mg/l of hygromycin. After the developed roots were transferred into soil, and PCR technique was used to confirm the presence of IFNγ-bar in the genomic of plants. Dot blot analysis was applied to detect IFNγ-bar protein in transgenic of to tobacco plants.
Maryam Ahmadi, Mustafa Valizadeh, Mahmoud Tourchi, Mohammad Moghaddam Vahed, Hossein Mohammadzadeh Jalaly,
Volume 1, Issue 1 (5-2014)
Abstract
For evaluation of genetic diversity among improved alfalfa varieties and Iranian landraces, 12 populations including five improved varieties (Kaysari, Kadi, Ranger, Mesmir, Seariver) and seven landraces (Gharayonje, Amozeynadin, Rahnani, Tazekand, Shazand, Hamedani, Yazdi) were evaluated using agronomic traits and enzyme markers. Thirty-five individuals of each variety were grown and analyzed in separate pots in a unbalanced completely randomized design (CRD). Analysis of variance for agronomic traits showed significant differences for most of the traits among improved and landrace varieties. For esterase and peroxidase enzymes based on presence or absence of enzyme bands (1, 0) eleven polymprphic isozyme bands were detected. For improved and landrace varieties Shanon index mean was 0.48 ± 0.246 and 0.519 ± 0.193, respectively, furthermore Nei genetic diversity index mean for improved and landraces was 0.327 ± 0.181 and 0.352 ± 0.148 respectively, suggesting no difference between improved and landrace varieties was found. Analysis of relation between isozyme markers and agronomic traits showed that there are significant differences between the presence of POX-4 and wet and dry yield in improved varieties.
Maryam Javedan Asl, Hamid Rajabi Memari, Daryoosh Nabati Ahmadi, Afrasiyab Rahnama Ghahfarokhi,
Volume 2, Issue 1 (5-2015)
Abstract
Yarrow (Achillea millefolium) is an herbaceous and perennial plant species which belongs to the Asteraceae family. Yarrow's essential oil has different compounds of monoterpene and sesquiterpens, which its main constituents are pinene and linalool. These compunds have anti-microbial and anti-pest activities and also can be used in the food industry, perfumery and cosmetics.The aim of the present study was to use the degenerate primers approach in order to isolate Pinene synthase and linalool synthase genes from Yarrow plant. Up to date, there is no any report on the availability of these genes in the world gene bank. In this investigation the total RNA was extracted from Yarrow then pinene synthase and linalool synthase genes were isolated, using degenerate primers and Polymerase Chain Reaction (PCR). PCR amplified two bands of 250 bp and 720 bp. The sequence data were compared with NCBI gene bank data. The results of diversity study among varieties and families based on Pis and Lis genes showed most similarity between Achillea and Artemisia plants. Also this similarity was seen between Asteraceae and Lamiaceae families and these families grouped together same group. These results also showed a relatively high similarity of Pis and Lis with some other plants which confirmed sequencing data.
Marziyeh Shazdehahmadi, Mahin Kharrazi,
Volume 2, Issue 2 (3-2016)
Abstract
Determination of genetic diversity of breeding material is the first step in breeding programs. Evaluation of tobacco genetic diversity is essential for breeding programs and preservation of genetic resources. Genetic diversity level in tobacco genotypes, is very important for selection of parents in breeding programs. In this study, genetic diversity of 100 tobacco genotypes was evaluated using 25 ISSR markers. Banding pattern based on the presence or absence of the bands showed with 0 and 1, respectively. Out of 237 fragments produced in total cultivars, 195 bands were polymorphic and average of polymorphism ranged from 4 to 12 per primer. Average of polymorphism percentage was 94.10. To determine the efficiency of ISSR markers, PIC and their polymorphic percentage was calculated. UBC 818, UBC 812 and UBC 815 had the best marker parameters and were introduced as the best primers for assessment of genetic diversity. In order to evaluate the genetic similarity between cultivars, different similarity coefficient (SM, Dice and Jaccard) was calculated and Mantel corresponding test was performed. Finally, dendrogram was drawn based on SM similarity coefficient and UPGMA algorithm and the Cofenetic coefficient was calculated. All genotypes formed two distinct clusters indicating the high efficiency of used primers in amplification the approximate parts of the genome. The principle coordinate analysis showed that the first three components could explain 79.65 % of total variance. Totally, evaluation the tobacco genetic diversity using ISSR markers is suitable and ISSR marker can be used as appropriate marker system to identify the diversity and genetic relationship for breeding programs of this plant.
Zahra Hajiahmadi, Reza Shirzadian-Khorramabad2, Mahmood Kazemzad, Mohammad Mahdi Sohani,
Volume 4, Issue 2 (3-2018)
Abstract
Tomato leafminer (Tuta absoluta) is one of the important pests of tomato in Iran. It causes serve losses to tomato yield between 50 to 100% in the world. The cryIAb gene has been introduced into many plant species, including maize resulting in protection of the maize plants against corn borer larvae. In most studies, constitutive promoters such as CaMV35S were employed for genetic transformation; however the constitutive expression of genes led to changes in plant metabolic pathways due to permanent energy consumption in plants. Since, wound inducible promoter MPI (Maize Protease Inhibitor) posses more efficiency and strength than CaMV35S promoter. Therefore, in the current study, transgenic tomato (cv. Falat) plants harboring cryIAb gene under control of the MPI promoter were developed for the first time. The MPI promoter was isolated from maize and cloned into pPZP122 expression vector replacing the CaMV35S promoter. The cryIAb gene was isolated from pCIB4427 and cloned in pPZP122:MPI:cryIAb and the resulting construct was transformed into Agrobacterium AGL1 strain using In planta approach. Initial selection of the transgenic plants was carried out in media culture containing gentamicin. PCR analysis confirmed the presence of transgene in gentamycin-resistance plants in the first and second generations by rate of 62.5% and 75.58%, respectively. Protein dot blotting using anti-CryIAb polyclonal antibody confirmed the presence of protein in the second generation of transgenic lines. Based on the result of Tuta bioassay, transgenic plants demonstrated an enhanced resistance against Tuta. Thus, the wound inducible promoter MPI can be used in genetic transformation of crop plants if insecticidal protein-encoding genes (such as different types of cry) are used and therefore, it is important to be used when plants asked to express only when are being attacked by insect pests.
Saman Valizadeh, Ahmad Ismaili, Hadi Ahmadi, Omid Ali Akbarpour, Bijan Bajalan, Ashkboos Amini,
Volume 6, Issue 2 (3-2020)
Abstract
Wheat is mostly cultivated at rainfed condition in Iran, so, water deficit stress has much effect on yield reduction. Hence, breeding activities are necessary for introduction of wheat tolerant genotypes to water deficit stress. In order to estimate the heritability and genetic correlation between traits of 36 wheat genotypes, an experiment was conducted in two separate conditions (water stress and non-stress) based on a randomized complete blocks design with three replications. Studied traits in wheat genotypes under water stress and normal condition showed significant differences for environment, genotype and genotype× environment interaction at 1 and 5% level of probability. The results of the factor analysis showed that the 6 first factor in normal condition explained 81.13% of total variance, and the 5 first factor in stress condition explained 74.96% of total variance. Estimation of genetic correlations based on REML approach revealed that biological yield, harvest index and number of grains per spike had the highest correlation with grain yield and these characteristics are of important for selecting the varieties with high yield under non-stress and stress conditions. Estimation of heritability based on REML approach showed that number of days to heading had the highest amount of heritability in both normal and stress conditions.
Ali Dowlatshah, Ahmad Ismaili, Hadi Ahmadi, Karim Khademi, Daryoush Goudarzi,
Volume 7, Issue 2 (3-2021)
Abstract
Plant breeding researches is based on genetic diversity and evaluation of genetic diversity is also one of the most important steps in introduction of new cultivars. In this study, genetic diversity of 25 grass pea genotypes was studied based on randomized complete block design with three replicates in Khorramabad (Iran). Analysis of variance showed significant differences among genotypes for most of traits. Mean comparison showed that genotype IF1312 with the highest grain yield and genotypes IF1332 and IF471 with the highest dry and fresh forage yield had the best yield. Principal component analysis showed that the first 3 factors explained 62.64% of total variance. Based on cluster analysis, genotypes IF1307, IF1872 and IF471 with the highest grain and forage yield are belonged to one cluster. REML method was used to estimate genetic correlation and heritability of different traits. The highest amount of heritability (0.87) was estimated for number of immature grains and the least heritability (0.10) was estimated for total dry weight. Grain yield had a high and positive genetic correlation with forage yield, and biomass, percentage of leaf and dry forage yield also had a high and positive genetic correlation with fresh forage yield. Totally, genotype IF1307 had the best performance for most of traits compared to the other genotypes and had an acceptable forage yield among genotypes.
Ali Barzgari, Saeed Malekzade Shafaroudi, Saeed Khavari Khorasani, Farajollah Shahriari Ahmadi,
Volume 8, Issue 2 (3-2022)
Abstract
In breeding programs determination of gene effects and general and specific combining ability for screening of test crosses is necessary. In order to estimate the genetic variance components and the general and specific combining ability of sweet corn lines, an experiment was conducted using 8 sweet corn S6 inbred lines (including 4 maternal and 4 paternal lines) by line × tester mating design in 2019, at the Agricultural and Natural Resources Research and Education Center of Khorasan Razavi Province, Mashhad, Iran. The obtained test cross hybrids were evaluated in a randomized complete block design with 3 replications in 2020. The results of line, tester and line × tester analysis for most of measured traits showed significant differences (p < 0.05). The σ2gca/σ2sca ratio for grain yield was equal to 0.1, showed that while both additive and dominance effects play a role in controlling this trait, but dominance effect was higher. The results for general combining ability of L3 and T1 lines showed positive and significant GCA effect for grain yield. Also, the specific combining ability of grain yield showed that T4 × L2, T1 × L3 and T3 × L1 had the highest SCA rate. In this study, in terms of grain yield, T1 × L3, T4 × L2 and T1 × L4 with 33.96, 30.47 and 27.85 tons per hectare had the highest green ear yield, respectively. These combinations can be as the hybrids with high yield potential in advanced breeding programs for release of new sweet corn varieties.
Jamshid Moradpour, Hadi Ahmadi, Mahmoud Bagheri, Daryoush Goudarzi,
Volume 9, Issue 1 (9-2022)
Abstract
Eggplant (Solanum melongena L.) has a high genetic variation in Iran and there are many landraces of this crop in Iran. In the present study, 15 superior genotypes of eggplant which were selected from Minab landraces accompanying two superior mother landraces (totally 17 lines) were studied for two successive years in three regions of Iran including Minab, Karaj and Jiroft. The experiment was conducted in Randomized complete block design with three replications. Finally, total yield of both years was measured and the combined analysis was done and the best line(s) for different climates were introduced using evaluation the stability of the lines via AMMI and GGE biplot procedures. Based on the results of means comparison of yield in the studied lines in each region from average of two years, GHE12 line in Minab region, SA13 line in Jiroft region and AM4, SA15 and SA5 lines in Karaj region have higher fruit yield than the other lines. Based on the results of yield comparison of the examined genotypes in each region from the average of two years of testing, GHE12 genotype in Minab region, SA13 genotype in Jiroft region and AM4, SA15 and SA5 genotypes in Karaj region had acceptable yield compared to other genotypes. However, according to the results of special adaptability and stability analysis, Y genotype for Minab region, SA13 genotype for Jiroft region and AM4 genotype for Karaj region are recommended
Jafar Ahmadi, Amir Abbas Taghizadeh, Mohialdin Pirkhezri,
Volume 9, Issue 2 (3-2023)
Abstract
To determine the ideal genotypes in terms of the quantity and quality of fruit, 32 genotypes of greengage were evaluated in a randomized complete block design with three replications at the research station of Horticultural Research Institute in Karaj during two cropping seasons 2018 and 2019. To evaluate the genotypes and to determine the ideal genotype, 26 traits related to fruit, fruiting and yield were used. Combined variance analysis of data showed that the genotype source of variation was significant for all studied traits at the level of 0.01 probability level. ASIIG index showed that the genotypes CodR, Cod100, Malayer, and Gojeh Siah are the best and most desirable genotypes with an index higher than 55%, respectively. Also, Cod98, Cod99, Gojeh Baghi Qasr Dasht, Gojeh Qomi, and Gojeh Holandi with ASIIG index higher than 49% were ranked next. The grouping of genotypes using ASIIG method diagrams, the genotypes CodR, Cod100, Cod98, Cod99, Gojeh Malayer, Gojeh Siah, and Gojeh Baghi Qasr Dasht were located in the ideal quartile. Using cluster analysis, the genotypes CodR, Cod100, black, and Malayer as well as completely ideal hypothetical genotype (+) were placed in a group at a distance line of 0.018. According to the results of this study, seven genotypes CodR, Cod100, Cod98, Cod99, Gojeh Malayer, Gojeh Siah, and Gojeh Baghi Qasr Dasht were selected as the ideal genotypes in terms of fruit quantity and quality. Finally, due to the high benefits of the ASIIG index, it was suggested that this index can be used in other horticultural products to determine the ideal genotype.
Khadijeh Abbaszadeh, Reza Shirzadian-Khorramabad, Mohammad Mahdi Sohani, Zahra Hajiahmadi,
Volume 9, Issue 2 (3-2023)
Abstract
Salinity stress affects morpho-physiological and biochemical traits of plants. The transgenic Bt plants play a significant role in pest control, but their response and ability to cope with environmental stresses still need to be evaluated. Therefore, effect of salinity stress at 0, 50, 100, 150, and 200 mM on morphological, physiological, and molecular traits of T3 transgenic tomato plants containing cry1Ab gene (CH-Falat-Bt) was investigated and compared with that of the non-transgenic control (CH-Falat). Evaluation of the morphological traits (leaf area, root length, fresh and dry weight of roots) at different salinity levels revealed that CH-Falat-Bt transgenic plants are more tolerant to salinity stress compared to CH-Falat non-transgenic plants. The chlorophyll content at 150 and 200 mM salinity levels was 12 and 9% plants, respectively. Moreover, the amount of RWC, carotenoids, proline and soluble sugars increased significantly in transgenic plants as salinity levels increased. The relative expression of SOS1 and SOS2 genes showed a significant increase in all salinity levels in CH-Falat-Bt transgenic plants compared to CH-Falat non-transgenic plants. The amount of electrolyte leakage in the transgenic plants was significantly reduced compared to the non-transgenic plants. The results of morphological, physiological, and molecular investigations of CH-Falat-Bt transgenic plants confirmed that the undesirable effects of salinity stress on transgenic plants is much less than non-transgenic ones. in general CH-Falat-Bt transgenic plants are more tolerant to different applied salinity levels than the wild variety.
Mohammad Nader Ebrahimi, Hadi Ahmadi, Mostafa Darvishnia, Daryoush Ghoudarzi,
Volume 10, Issue 2 (2-2024)
Abstract
Hexaploid winter wheat (Triticum aestivum L., 2n= 6x= 42, AABBDD) is an important small-grain cereal crop grown for food and feed. In Iran, wheat is the most cultivated cereal crop where winter wheat is ranked 1st in terms of production. Wheat fusarium head blight (FHB) is one of the most important diseases of wheat in humid and hot regions of the world, which causes a decrease in yield and grain quality. Fusarium head blight is caused by Fusarium graminearum. In order to investigate the resistance of some wheat traits against HBF, 27 varieties and lines of bread wheat were sown at the farm of Faculty of Agriculture, Lorestan University. The spikes were infected at the first flowering stage by spray inoculation. The results showed that there was a great diversity among the 27 varieties of bread wheat tested in terms of growth traits, yield components, and grain yield. The results of The Duncan's test for comparing means showed that cultivars Ghods, Shiraz, Morvarid 2, and Pishtaz had a significant superiority in terms of grain yield compared to other cultivars. The results of simple correlation and also stepwise regression analysis showed that the traits of 1000 seed weight, plant height, number of seeds per spike, number of spikes, spike length and plant height are very important traits for grain yield. According to values of broad sense heritability and genetic gain for plant height and high correlation of this trait and seed yield, plant height can be used as a suitable trait for improving seed yield by selection. The overall results showed that Shiraz, Pishtaz, Quds and Morwarid-2 cultivars are suitable for transferring resistance to wheat spike blight disease in breeding programs.
Forough Joudaki, Ahmad Ismaili, Seyed Sajad Sohrabi, Seyedeh Zahra Hosseini, Hadi Ahmadi,
Volume 10, Issue 2 (2-2024)
Abstract
Gall oak (Quercus infectoria) is one of the extraordinary tree species with functional medicinal properties within the oak family. Various studies have confirmed the presence of numerous secondary metabolites with therapeutic properties in this plant. Despite the significance of gall oak, its genetic structure remains elusive. Therefore, unraveling the genetic structure of gall ok may provide valuable insights into its potential applications across diverse industries. MicroRNAs emerge as pivotal genetic elements implicated in the biosynthesis of crucial metabolites across a wide range of different plant species. Despite the significant role of miRNAs in plants, as of yet, no miRNAs have been reported in Q. infectoria.. Therefore, in the present study, after assembling the transcriptome of Q. infectoria, the conserved microRNAs were identified. Leaf and root samples of Q. infectoria were collected from trees in the Shineh region, and 2-year-old seedlings were grown from mature oaks in Khorramabad (Lorestan Province, Iran). Total RNA was extracted from roots and leaves using the Djami-Tchatchou method. After sequencing by the Illumina HiSeq 2500 platform and checking the quality of all the generated reads, the adapter sequences were removed, and the high-quality reads were assembled using Trinity package. To identify miRNAs and their target genes, all plant miRNAs sequences were downloaded from the miRbase database. The BLASTn algorithm was employed to identify the highest similarity between unigenes and mature plant miRNAs. Furthermore, BLASTx was used to search against the non-redundant proteins (NR) database to remove protein-coding unigenes. The investigation of miRNA second-structure prediction involved assessing the similarity between potential unigenes and mature miRNA sequences using the mfold web tool. Identification of miRNA target genes and gene ontology (GO) was performed using the psRNAtarget web-tool and OmicsBox software, respectively. Following a range of strict filtering criteria, four miRNAs belonging to conserved miRNAs families were identified, including qin-miR156, qin-miR399, qin-miR160, and qin-miR172. KEGG pathway analysis showed the target genes were involved in the citrate cycle pathway. Examining miRNA target genes in Q. infectoria and analyzing their interaction network, finally led to the identification of three hub genes. Identified miRNA target genes were associated with the biosynthesis of various enzyme groups, suggesting that most of miRNAs regulating hydrolases, transferases, and oxidoreductases. Given the role of microRNAs in regulating transcription factors and their impact on genes involved in secondary metabolite biosynthesis, future breeding programs in Q. infectoria may benefit from the potential of such regulatory elements as a guide and key.
Fatemeh Bagherzadeh, Hannaneh Mirahmadi, Soraya Pourtabrizi, Ali Kazemipour, Maryam Dorraninejad, Roohollah Abdoshahi,
Volume 11, Issue 1 (9-2024)
Abstract
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