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Showing 2 results for Abdollahi Mandoulakani
Fariba Ranjbar, Babak Abdollahi Mandoulakani, Raheleh Ghasemzadeh,
Volume 10, Issue 1 (9-2023)
Abstract
To evaluate the expression pattern of genes encoding antioxidant enzymes catalase, ascorbate peroxidase and polyphenol oxidase under iron deficiency conditions in Fe- efficient (Pishtaz) and -inefficient (Falat) bread wheat cultivars, a CRD (completely randomized design) based factorial experiment was conducted with three replications. The cultivars were grown under iron deficiency (Less than 1.5 mg Fe/kg soil) and compared with normal conditions (10 mg Fe/kg soil). The relative expression levels of the above-mentioned genes were measured using Real-time PCR technique in the leaves and roots of the cultivars at two growth stages: vegetative (one month after germination) and reproductive (30% of heading). The results revealed a remarkable enhancement in calatalse expression in the roots of both cultivars in the vegetatative stage but it was higher in Fe-efficient cultivar than -inefficient one. The expression of this gene was decreased in leaves at the same stage as well as in the roots of both cultivars in the vegetative stage. The expression level of ascorbate peroxidase gene in the reproductive stage in the roots of Fe-inefficient cultivar was higher than that of -efficient one. In the vegetative stage, the expression of this gene increased in the leaves and roots of Fe-efficient cultivar, but it was decresed in Fe-inefficient cultivar. The relative expression level of polyphenol oxidase gene in the vegetative stage under iron deficiency conditions in the leaf increased almost three times, compared to the roots, while the expression of this gene decreased in the reproductive stage in both leaves and roots. By increasing the expression of both catalase and ascorbate peroxidase genes in the roots of both cultivars in the reproductive stage under iron deficiency conditions, it seems that bread wheat cultivars might reduce the deletrious effects of stress and maintain yield through transferring much iron to the seeds in the seed filling stage. The findings of the present study may increase our understanding of the important role of genes encoding antioxidant enzymes in Fe deficiency stress conditions.
Fatemeh Asadzadeh, Babak Abdollahi Mandoulakani,
Volume 11, Issue 1 (9-2024)
Abstract
To investigate the effect of iron deficiency stress on the expression of genes encoding bZIP4, bZIP79, and bZIP97 transcription factors in iron-efficient and -inefficient bread wheat cultivars, a factorial experiment was conducted in a completely randomized design with three replications in the research greenhouse of Urmia University. Falat (iron-inefficient) and Pishtaz (iron-efficient) cultivars were grown in iron deficiency and sufficiency conditions. The expression levels of genes mentioned above were measured using real time PCR technique in the leaves and roots of the cultivars at two growth stages: one month after germination (vegetative) and 30% of spiking (reproductive). The results revealed the highest increase in the relative expression of bZIP79 (more than 14-fold change) and bZIP97 (more than 3-fold change) in the leaves of iron-inefficient (Falat) and -efficient (Pishtaz) cultivars, respectively, at vegetative stage. The highest relative expression of bZIP4 was observed in the roots of iron-inefficient cultivars in the vegetative stage. This probably shows that bZIP4 might activate the transcription of the genes responsible for iron uptake from the soil. Increased expression of bZIP79 in the leaves of iron-efficient cultivar in the vegetative stage under iron deficiency conditions, indicates the involvement of this transcription factor in the activation of genes responsible for iron transfer from the leaves to the grain and other tissues. In general, this research helps understand the mechanism of plants coping with iron deficiency stress. Also, the identification of key bZIP transcription factors involved in the activation of genes responsible for iron absorption and transport in bread wheat plants provides the possibility of genetic manipulation of bread wheat cultivars to produce cultivars with a higher amount of iron in the grain
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