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Showing 4 results for Abdollahi
Azadeh Souri, Asghar Mirzaie-Asl, Leila Khodaei, Mohammad Reza Abdollahi,
Volume 6, Issue 2 (3-2020)
Abstract
Autumn sowing of sugar beet is a suitable way in sustainable agriculture. Bolting is an undesirable phenomenon which reduces sugar beet yield and it is the most important limiting factor in autumn sowing of sugar beet. Identification and comparison of the sequence of flowering genes in various genotypes can help to understand the molecular mechanisms controlling bolting. In the previous studies, it was revealed that expression level of FT1 and VIN3 genes in sugar beet is associated with bolting resistance. In this study, the sequence of FT1 gene promotor and three versions of VIN3 gene promoters of sugar beet were compared in three bolting resistant and three bolting susceptible genotypes. Primer design for each gene was carried out using the DNA sequences found at the NCBI database. DNA was extracted from leaf samples growing in pots and was used as template in PCR reactions. Similar length of amplified fragments for each promoter gene in bolting susceptible and bolting resistant genotypes were selected and sequenced for more accurate assessment. There was no mutation in the FT1 gene promoter, however 624 substitution and insertion/deletion mutations were observed in the promoter of three versions of VIN3 gene. A 228-bp ins/del region was detected in the VIN3-like1 promoter. This region contains promoter elements and seems to have a control function. Comparison of detected mutations between susceptible and resistant genotypes did not show a distinct pattern for bolting.
Razieh Azizian Mosleh, Mohammad Reza Abdollahi, Hassan Sarikhani, Asghar Mirzaie-Asl, Payam Pour Mohammadi,
Volume 7, Issue 2 (3-2021)
Abstract
Optimization of in vitro methods for the production of maize double haploids plays an important role in the breeding programs of this plant. In this study, the effects of 5-azacytidine on agronomic traits, androgenesis induction efficiency and also, DNA methyltransferase gene expression (AF229183.1) in two growth stages of maize were investigated. This experiment was performed as factorial based on a completely randomized block design with three replications. Two maize genotypes (DH5 × DH7 and ETMH-82) were considered as the first factor and treatment of maize seeds with 5-azacytidine (0, 5, 10, and 100 μM) was considered as the second factor. The maize seeds were sowed in the field and during the growth stages, various morphological and agronomic traits were recorded. In the anther culture experiment, the suitable anthers containing microspores at mid to late-uninucleate stages were selected and cultured in an YPm culture medium containing 1 mg/l 2, 4-D, and 2 mg/l BAP. Interaction effects of genotype and 5-azacytidine concentrations showed significant differences for the majority of studied traits except for number of kernel per ear row, kernel depth, plant diameter, number of leaves and number of ears. The highest amounts of 1000-kernel weight were obtained with treatments of 10 and 100 μM and the highest ones for grain yield and biological yield traits were obtained with 100 μM 5-azacytidine treatment for both genotypes. Seeds of DH5 × DH7 genotype treated with 5 μM 5-azacYtidine produced the highest mean number of embryo-like structures (0.1833) and regenerated plantlets (0.067) per each anther. Relative expression of DNA methyltransferase gene in maize seeds treated with different concentrations of 5-azacytidine showed a significant decrease in both genotypes and both growth stages compared to control plants (treated with 0 μM 5-azacytidine), that this decrease in gene expression could lead to improved androgenesis induction in anther culture of DH5 × DH7 genotype. However, despite the decrease in expression of this gene in two growth stages of ETMH-82 genotype, androgenesis induction was not observed in this genotype. The results of the present study can help to determine the role of epigenetic factors in androgenesis induction and improving the production of haploid plants in maize.
Fariba Ranjbar, Babak Abdollahi Mandoulakani, Raheleh Ghasemzadeh,
Volume 10, Issue 1 (9-2023)
Abstract
To evaluate the expression pattern of genes encoding antioxidant enzymes catalase, ascorbate peroxidase and polyphenol oxidase under iron deficiency conditions in Fe- efficient (Pishtaz) and -inefficient (Falat) bread wheat cultivars, a CRD (completely randomized design) based factorial experiment was conducted with three replications. The cultivars were grown under iron deficiency (Less than 1.5 mg Fe/kg soil) and compared with normal conditions (10 mg Fe/kg soil). The relative expression levels of the above-mentioned genes were measured using Real-time PCR technique in the leaves and roots of the cultivars at two growth stages: vegetative (one month after germination) and reproductive (30% of heading). The results revealed a remarkable enhancement in calatalse expression in the roots of both cultivars in the vegetatative stage but it was higher in Fe-efficient cultivar than -inefficient one. The expression of this gene was decreased in leaves at the same stage as well as in the roots of both cultivars in the vegetative stage. The expression level of ascorbate peroxidase gene in the reproductive stage in the roots of Fe-inefficient cultivar was higher than that of -efficient one. In the vegetative stage, the expression of this gene increased in the leaves and roots of Fe-efficient cultivar, but it was decresed in Fe-inefficient cultivar. The relative expression level of polyphenol oxidase gene in the vegetative stage under iron deficiency conditions in the leaf increased almost three times, compared to the roots, while the expression of this gene decreased in the reproductive stage in both leaves and roots. By increasing the expression of both catalase and ascorbate peroxidase genes in the roots of both cultivars in the reproductive stage under iron deficiency conditions, it seems that bread wheat cultivars might reduce the deletrious effects of stress and maintain yield through transferring much iron to the seeds in the seed filling stage. The findings of the present study may increase our understanding of the important role of genes encoding antioxidant enzymes in Fe deficiency stress conditions.
Fatemeh Asadzadeh, Babak Abdollahi Mandoulakani,
Volume 11, Issue 1 (9-2024)
Abstract
To investigate the effect of iron deficiency stress on the expression of genes encoding bZIP4, bZIP79, and bZIP97 transcription factors in iron-efficient and -inefficient bread wheat cultivars, a factorial experiment was conducted in a completely randomized design with three replications in the research greenhouse of Urmia University. Falat (iron-inefficient) and Pishtaz (iron-efficient) cultivars were grown in iron deficiency and sufficiency conditions. The expression levels of genes mentioned above were measured using real time PCR technique in the leaves and roots of the cultivars at two growth stages: one month after germination (vegetative) and 30% of spiking (reproductive). The results revealed the highest increase in the relative expression of bZIP79 (more than 14-fold change) and bZIP97 (more than 3-fold change) in the leaves of iron-inefficient (Falat) and -efficient (Pishtaz) cultivars, respectively, at vegetative stage. The highest relative expression of bZIP4 was observed in the roots of iron-inefficient cultivars in the vegetative stage. This probably shows that bZIP4 might activate the transcription of the genes responsible for iron uptake from the soil. Increased expression of bZIP79 in the leaves of iron-efficient cultivar in the vegetative stage under iron deficiency conditions, indicates the involvement of this transcription factor in the activation of genes responsible for iron transfer from the leaves to the grain and other tissues. In general, this research helps understand the mechanism of plants coping with iron deficiency stress. Also, the identification of key bZIP transcription factors involved in the activation of genes responsible for iron absorption and transport in bread wheat plants provides the possibility of genetic manipulation of bread wheat cultivars to produce cultivars with a higher amount of iron in the grain.
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