Identification of Drought Stress Responsive Proteins in Susceptible Genotype of Chickpea (Cicer arietinum L.)

Shabani, Akbar; Zebarjadi, Alireza; Mostafaei, Ali; Mohsen, Saeidi; Poordad, Seyad Saeid

doi:10.29252/pgr.3.1.1

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Volume 3, Issue 1 (2016)

pgr 2016, 3(1): 1-12

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Identification of Drought Stress Responsive Proteins in Susceptible Genotype of Chickpea (Cicer arietinum L.)

Akbar Shabani

, Alireza Zebarjadi ^*

, Ali Mostafaei

, Saeidi Mohsen

, Seyad Saeid Poordad

Associate Professor, Department of Agronomy and Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran

Abstract: (18529 Views)

Plants are capable of responding to environmental stresses by activating their adaptation mechanisms and their response to environmental factors by changing their gene expression. Drought stress is considered as the most important abiotic stress in agriculture. In this regard, in present research, proteomics techniques used to detect proteins were responding to drought stress. To select drought susceptible genotype, 64 chickpea genotypes were assessed by simple lattice design 8×8 at the Sararood station (Iran) and then in the greenhouse of College of Agriculture and Natural Resources of Kermanshah Razi University (Iran) in three levels of stress including normal, medium and intensive stress conditions at poding stage. Finally, SAR 80 JI 09 K12-8 genotype was selected as susceptible to drought stress. Then, the evaluations consisted of a leaf proteome induced under drought stress conditions were performed. To study and identify the proteins associated with drought, total protein was extracted from the leaves by TCA- acetone method and isolated in the first dimension by IPG gels with pH gradient 7-4 and in second dimension after by 12.5% concentration polyacrylamide gels. Therefore, in the drought susceptible genotype the value of each spot was used as a standard amount. Protein spots on the gel were scanned and identified by using Image Master 2D Platinum of Melanie 6.0 software. The results of two-dimensional gel analysis and protein identification of drought susceptible genotypes showed that leaf proteome pattern has been widely changed in drought stress condition. In susceptible genotype, 212 protein spots repeatable were identified. 10 spots were detected by using MALDI-TOF-TOF mass spectrometry which were divided in different groups based on response to drought stress in biological cycles.

Keywords: Two-dimensional electrophoresis, Proteomics, Drought stress, Chickpea

Full-Text [PDF 849 kb] (3599 Downloads)

Type of Study: Research | Subject: Plant improvement

References

1. Aranjueli, I., Molero, G., Erice, G., Avice, J.C. and Nogues, S. (2011). Plant physiology and proteomics reveals the leaf response to drought in alfalfa (Medicago sativa L.). Journal of Experimental Botany, 62(1): 111-123.

2. Ashraf, N., Ghai, D., Barman, P., Basu, S., Gangisetty, N., Mandal, M.K., Chakraborty, N., Datta, A. and Chakraborty, S. (2009). Comparative analyses of genotype dependent expressed sequence tags and stress-responsive transcriptome of chickpea wilt illustrate predicted and unexpected genes and novel regulators of plant immunity, BMC Genomics. 10, 415.

3. Bhushan, D., Pandey, A., Choudhary, M. K., Datta, A., Chakraborty, S. and Chakraborty, N (2014). Comparative Proteomics Analysis of Differentially Expressed Proteins in Chickpea Extracellular Matrix during Dehydration Stress. Molecular & Cellular Proteomics, 6(111): 868-885.

4. Bloom, H., Beier, H. and Gross, H. S. (1987). Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis, 8, 93-99.

5. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-Dye binding. Analytical Biochemistry, 72: 248-254.

6. Brown, E.N., Friemann, R., Karlsson, A., Parales, J.V., Couture, M.M., Eltis, L.D. and Ramaswamy, S. (2008). “Determining Rieske cluster reduction potentials Journal of Biological Inorganic Chemistry, 13(8): 1301-1313.

7. Caruso, G., Cavaliere, C., Guarino, C., Gubbiotti, R., Foglia, P. and Lagana, A. (2009). Identification of change in Triticom durum L. leaf proteom in response to salt stress by twodimensional electrophoresis and MALDI-TOF mass spectrometry. Analytical and Bioanalytcal Chemistry, 391: 381-390.

8. Chen, A. P., Wang, G. L., Qu, Z. L., Lu, C.X., Liu, N., Wang, F. and Xia. 2007. Ectopic expression of ThCYP1, a stress-responsive cyclophilin gene from Thellungiella halophila, confers salt tolerance in fission yeast and tobacco cells. Plant Cell Reports, 26: 237-245.

9. Chowdhry, M.A., Ambreen, A. and Khaliq, I. (2002). Genetic control of some polygenic traits in aestivum species. Asian Journal of Plant Science, 1: 235-237.

10. Damerval, C., De Vienne, D., Zivy, M. and Thiellement, H. (1986). Technical improvments in twodimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins. Electrophoresis, 7: 52-54.

11. Di Carli, M., Zamboni, A., Enrico, P. M., Pezzotti, M., Lilley, S., Benvenuto, E. and Desiderio, A. (2011). Two-dimensional differential in gel electrophoresis (2D-DIGE) analysis of grape berry proteome during post-harvest withering. Journal of Proteome Research, 10(1): 429-446.

12. Gao, L., Yan, X., Li, X., Guo, Y., Hua, G., Ma, W. and. Yan, Y. (2011). Proteome analysis of wheat leaf under salt stress by two–dimensional difference gel electrophoresis (2D-DIGE). Phytochemistry, 72(10): 91-1180.

13. Gupta, S.C., Sharma, A., Mishra, M., Mishra, R. and Chowdhouri, D.K. (2010). Heat shock proteins in toxicology: How close and How far? Life Sciences, 86: 377-384.

14. Hajheidari, M., Abdollahian-Noghabi, M., Askari, H., Heidari, M., Sadeghian, S. Y., Ober, E.S. and Salekdeh, G.H. (2005). Proteome analysis of sugar beet leaves under drought stress. Proteomics, 5(4): 950-60.

15. Hashimoto, M. and Komatsu, S. (2007). Proteomic analysis of rice seedlings during cold stress. Proteomics, 7(8): 1293-302.

16. Hooper, P.L. and Hooper, J.J. (2005). Loss of defense against stress: Diabetes and heat shock proteins. Diabetes Technology & Therapeutic, 7: 204-208.

17. Juliann, G., Kiang, G. and Tsokos, C. (1998). Heat shock protein 70 Kda: Molecular Biology, Biochemistry, and Physiology. Pharmacology & Therapeutics, 80(2):183-201.

18. Kawasaki, S., Borchert, C., Deyholos, M. and Wang, H. (2001). Gene expression profiles during the Initial phase of salt stress in rice. Plant Cell, 13: 889-905.

19. Link, T.A. (1997). The role of the ‘Rieske’ iron sulfur protein in the hydroquinone oxidation (Q (P)) site of the cytochrome bc1 complex. The ‘proton-gated affinity change’ mechanism. FEBS Letters, 412 (2): 257-64.

20. Mehrabi, A.M. (2013). Effect of drought stress on protein profile of hexaploid wheat in heading stage. Ph.D. Thesis, Islamic Azad University, Science and Research Branch, Tehran, Iran (In Persian).

21. Rosegrant, M.W. and Agcaoili, M., (2010). Global food demand, supply, and price prospects to 2010. Washington, DC: International Food Policy Research Institute.

22. Seki, M., Narusaka, M., Ishida, J., Nanjo, T., Fujita, M., Oono, Y., Kamiya, A., Nakajima, MEnju, A. and Sakurai, T. (2002). Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. The Plant Journal, 31: 279-92.

23. Varshney, R.K., Song, C., Saxena, R.K., Azam, S., Yu, S., Sharpe, A.G., Cannon, S., Baek, J., Rosen, B.D., Tar’an, B., Millan, T., Zhang, X., Ramsay, L.D., Iwata, A., Wang, Y., Nelson, W. and Farmer, A.D. (2013). Draft genome sequence of chickpea (Cicer arietinum L.) provides a resource for trait improvement. Nature Biotechnology, 31(3): 240-249.

24. Wang, M.C., Peng, Z.Y., Li, C.L., Liu, C. and Xia, G.M. (2008). Proteomics analysis on a high salt tolerance introgression strain of Triticum aestivum. Proteomics. 8: 1470-1489.

25. Xu, J. and Giannakakou, P. (2006). Targeting microtubules for cancer chemotherapy. Current Medicinal Chemistry Anticancer Agents, 5 (1): 65-71.

26. Zhang, H., Pala, M., Oweis, T. and Harris, H. (2000). Water use and water-use efficiency of chickpea and lentil in a Mediterranean environment. Austeralian Journal of Agricultuare Research, 51: 295-304.

‎ 10.29252/pgr.3.1.1

‎ 20.1001.1.23831367.1395.3.1.1.0

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Shabani A, Zebarjadi A, Mostafaei A, Mohsen S, Poordad S S. Identification of Drought Stress Responsive Proteins in Susceptible Genotype of Chickpea (Cicer arietinum L.). pgr 2016; 3 (1) :1-12
URL: http://pgr.lu.ac.ir/article-1-85-en.html

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