[Home ] [Archive]   [ فارسی ]  
:: About :: Main :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..



 
..
:: Volume 1, Issue 2 (2015) ::
pgr 2015, 1(2): 15-24 Back to browse issues page
QTL Mapping of Phosphorus Concentration and Content on Shoot of Barley
Samira Khodaei , Seyed Abolghasem Mohammadi * , Behzad Sadeghzadeh
Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tabriz University, Tabriz, Iran
Abstract:   (26178 Views)
Phosphorus is one of the important macronutrients involved in various physiological and metabolic pathways. It has also major role in development and transmission of energy. To map QTLs for the traits associated with phosphorus accumulation at shoot stage in barley, 148 doubled haploid lines derived from a cross between Sahara3771 and Clipper cultivars were evaluated in greenhouse condition. Analysis of variance showed, that significant differences among the lines for all traits. Transgressive segregation was observed for all traits. Linkage map of population consist of 246 SSR, EST-SSR markers, 238 RFLP, 26 retrotransposone markers including IRAP, REMAP and a morphological marker that coverd 1099.09 cM of barley genome and an average distance of 2.15 cM between two adjacent markers. In total, 13 QTLs were identified for phosphorus concentration and content at five-leaf and maturity stages. For phosphorus concentration and content at five leaf stages three and two QTLs were found, respectively. Four QTLs were detected for phosphorus concentration at maturity stage. Out of which three and one QTL showed positive and negative additive effects, respectively. For phosphorus content of single plant at maturity stage, four QTLs explaining 60% of phenotypic variance were mapped. Out of 13 QTLs identified for the trait, 10 QTLs had positive additive effects, indicating the role of Clipper alleles in this loci in increasing the related traits value in offspring. In the present study, one common QTL identified which could be due to genetic linkage or pleiotropic effect.
Keywords: Barley, Phosphorus, Interval composite mapping, Retrotransposone marker
Full-Text [PDF 587 kb]   (2191 Downloads)    
Type of Study: Research | Subject: Plant improvement
Accepted: 2015/04/9
References
1. Bieleski, R. (1973). Phosphate pools, phosphate transport, and phosphate availability. Annual review of plant physiology, 24: 225-252.
2. Dai, F., Qiu, L., Ye, L., Wu, D., Zhou, M. and Zhang, G. (2011). Identification of a phytase gene in barley (Hordeum vulgare L.). PLoS ONE, 6: 1-8.
3. Ebadi, A. (2013). Construction of barley doubled haploid population microsatellite linkage map and identification of genetic regions associated with agronomic traits and some micronutrients accumulation. Ph.D. Thesis, Faculty of Agriculture, Tabriz University, Tabriz, Iran (In Persian).
4. Fageria, N., Baligar, V. and Li, Y. (2008). The role of nutrient efficient plants in improving crop yields in the twenty first century. Journal of plant nutrition, 31: 1121-1157.
5. Holford, I.C.R. (1997). Soil phosphorus its measurement and its uptake by plants. Australian Journal of Soil Research., 35: 227-239.
6. Hu, B., Wu, P., Liao, C., Zhang, W. and Ni, J. (2001). QTLs and epistasis underlying activity of acid phosphatase under phosphorus sufficient and deficient condition in rice (Oryza sativa L.). Plant and Soil, 230: 99-105.
7. Kalendar, R. and Schulman, A.H. (2007). IRAP and REMAP for retrotransposon-based genotyping and fingerprinting. Nature Protocols, 1: 2478-2484.
8. Kjaer, B. and Jensen, J. (1995). The inheritance of nitrogen and phosphorus content in barley analysed by genetic markers. Hereditas, 123: 109-119.
9. Lorieux, M. (2012). MapDisto: fast and efficient computation of genetic linkage maps. Molecular Breeding, 30: 1231-1235.
10. Lynch, J.P. (2007). Turner review no. 14. Roots of the second green revolution. Australian Journal of Botany, 55: 493-512.
11. Martin, J.H., Leonard, W.H. and Stamp, D.L. (1976). Principles of Field Crop Production, 3th edn, Collier Macmillan, Toronto, Canada.
12. Raghothama, K. (1999). Phosphate acquisition. Annual review of plant biology, 50: 665-693. Reiter, R.S., Coors, J., Sussman, M. and Gabelman, W. (1991). Genetic analysis of tolerance to low-phosphorus stress in maize using restriction fragment length polymorphisms. Theoretical and Applied Genetics, 82: 561-568.
13. Saghai-Maroof, M.A., Soliman, K., Tpregensen, R.A. and Allard, R.W. (1984). Ribosomal DNA spacer-lenth polymorphism in barley: Mendelian inheritance chromosomal location and population dynamics. Proceeding of the National Academy of Sciences of the United States of America. 81: 8018-8014.
14. Schachtman, D.P., Reid, R.J. and Ayling, S.M. (1998). Phosphorus uptake by plants: from soil to cell. Plant physiology, 116: 447-453.
15. Shi, R., Li, H., Tong, Y., Jing, R., Zhang, F. and Zou, C. (2008). Identification of quantitative trait locus of zinc and phosphorus density in wheat (Triticum aestivum L.) grain. Plant and Soil, 306: 104-95.
16. Su, J.-Y., Zheng, Q., Li, H.-W., Li, B., Jing, R.-L., Tong, Y.-P. and Li, Z.-S. (2009). Detection of QTLs for phosphorus use efficiency in relation to agronomic performance of wheat grown under phosphorus sufficient and limited conditions. Plant Science, 176: 824-836.
17. Su, J., Xiao, Y., Li, M., Liu, Q., Li, B., Tong, Y., Jia, J. and Li, Z. (2006). Mapping QTLs for phosphorus-deficiency tolerance at wheat seedling stage. Plant and Soil, 281: 25-36.
18. Wang, S., Basten, C.J. and Zeng, Z-B. (2012). Windows QTL Cartographer V2.5-011. Raleigh, NC: Department of Statistics, State University, North Carolina.
19. Wissuwa, M., Yano, M. and Ae, N. (1998). Mapping of QTLs for phosphorus-deficiency tolerance in rice (Oryza sativa L.). Theoretical and Applied Genetics, 97: 777-783.
20. Zhu, J., Kaeppler, S.M. and Lynch, J.P. (2005). Mapping of QTL controlling root hair length in maize (Zea mays L.) under phosphorus deficiency. Plant and Soil, 270: 299-310.
Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khodaei S, Mohammadi S A, Sadeghzadeh B. QTL Mapping of Phosphorus Concentration and Content on Shoot of Barley. pgr 2015; 1 (2) :15-24
URL: http://pgr.lu.ac.ir/article-1-32-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 1, Issue 2 (2015) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
Persian site map - English site map - Created in 0.05 seconds with 39 queries by YEKTAWEB 4642