:: Volume 10, Issue 2 (2024) ::
pgr 2024, 10(2): 79-90 Back to browse issues page
Karyological Varation in Different Populations of Matricaria Recutita L.
Hamideh Javadi * , Parvin Salehi Shanjani
Department of Natural Resources Gene Bank of Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization, Tehran, Iran , javadi@rifr.ac.ir
Abstract:   (822 Views)
German chamomile, Matricaria recutita L., is a medicinal plant of Asteraceae family, which is known as the "star" among medicinal species and its use dates back to the time of the Egyptian pharaohs. In this study, four populations of M. recutita that were collected from different parts of Iran, were studied for chromosome and karyotypic characteristics. Squash method with Hematoxylin staining was used to prepare microscopic slides. Microscopic observations showed the number of somatic chromosomes was 2n=18 for all populations. The length (TL, LA and SA) and symmetry karyotype (AR, CI, TF%, DRL, SC, LA% and SA%), as well inter and intra chromosome asymmetry (A1 and A2) were calculated. Karyotypic formula analysis showed the predominance of metacentric chromosomes in all populations. Based on mean of measured traits, the longest chromosome and genome belonged to population 15123 (Arak). According to Stebbins classification, populations were categorized in two groups. Karyotype symmetry factors showed that two populations 15123 (Arak) and 8959 (Borujen) had asymmetric and symmetric karyotypes, respectively. In PCA, the first three components could explain the entire diversity of populations. The most diversity of populations was for chromosome length traits. According to Ward's clustering method, the populations were placed in three groups at a genetic distance of 2.32. In general, the existence of high genetic and cytogenetic diversity indicates the existence of high potential in the improvement of Matricaria genus and cross-breeding activities. The data obtained from this research are used in the future breeding programs of Matricaria L.
Keywords: German chamomile, Cytogenetics, Chromosome, Matricaria recutita
Full-Text [PDF 2551 kb]   (243 Downloads)    
Type of Study: Research | Subject: Cytogenetics
Accepted: 2024/02/21
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