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Evaluation of MYB93 and MAD8 Genes in Transgenic and Non-Transgenic Rice
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Kobra Arab   , Rudabeh Ravash * , Behrouz Shiran |
| Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University , r.ravash@sku.ac.ir |
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Abstract: (11647 Views) |
Increasing drought tolerance, especially in rice, which is one of the most important crops in Asia, is necessary. Transcription factors are specific sequence DNA-binding proteins that are capable of activating or suppressing transcription. These proteins regulate gene expression levels by binding to cis regulatory elements in the promoter of target genes to control various biological processes such as growth, cell division and response to environmental stresses. In this study, MAD8 and MYB93 genes that were involved in drought stress in rice were considered in two leaf and anther tissues at 0, 24, 48, 72 h and one week after stopping irrigation. The results of q-PCR analysis showed significant expression changes of these transcription factor genes under drought stress conditions. In this study, a significant increase in the expression of these genes at 24 h after drought stress in transgenic plants (Promoter region with accession: NC_029264.1 and GUS gene have transformed) compared to non-transgenic plants showed a relationship between these transcription factors and higher expression of transported promoter in transgenic plants. |
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| Keywords: Rice, Abiotic stress, Transcription factors, MYB93, MAD8 |
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Full-Text [PDF 1271 kb]
(1444 Downloads)
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Type of Study: Research |
Subject:
Molecular genetics
Accepted: 2020/03/3
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| References |
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