[Home ] [Archive]   [ فارسی ]  
:: About :: Main :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..



 
..
:: Volume 7, Issue 2 (2021) ::
pgr 2021, 7(2): 97-108 Back to browse issues page
Application of SSR Markers for Genetic Segregation of Some Commercial Date Palm Cultivars
Mahdi Rezaei * , Abdoreza Kavand
Seed and Plant Certification and Registration Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran , meh.rezaee@areeo.ac.ir
Abstract:   (6628 Views)
Cultivar identification in micro-propagated date palm seedlings is laborious so that application of molecular markers to facilitate and acceleration of the procedure seems inevitable. Given the need for control the originality of micro- propagated date palm seedlings, the aim of this study was evaluation of SSR markers usability to cultivar identification in micro-propagated date palm seedlings. Original samples of Green Ghanami, Red Ghanami, Gantar, Deiry, Ostaemran, Barhi, Medjool, Zahedi and Piarum cultivars were used control. Taking into account the rigidity of leaves and subsequently high consumption of liquid nitrogen to powder leaves, an efficient method for powdering of leaves using Tissue Lyser II instrument was optimized. Eight SSR primer pairs were used for polymerase chain reaction. The Results showed that by using these molecular markers and reliable controls, determination of micro- propagated date palm cultivars is feasible. Clustering of cultivars showed that all of them were differentiated using five SSR primer pairs including mPdCIR025, mPdCIR057, mPdCIR070, PDAG1003 and DP175. Also, barcoding of scored band illustrated that c1 allele (230 to 240 bp) for Piarum cultivar and d3 allele (220 to 230 bp) for Medjool cultivar were exclusive. Totally to make the results referable, cultivar identification diagram was drawn up.
Keywords: Specific allele, Cultivar identification diagram, Micro-propagation, Molecular markers, Genetic fidelity
Full-Text [PDF 784 kb]   (1092 Downloads)    
Type of Study: Research | Subject: Molecular genetics
Accepted: 2021/01/6
References
1. Abd-Alla, M.M. (2010). Genetic stability on Phoenix dactylifera var. Karama produced in vitro. New York Science Journal, 3: 70-75.
2. Ahmed, T.A., Alsamaraee Zaidan, S.A. and Elmeer, K. (2012). Inter-simple sequence repeat (ISSR) Analysis of soma clonal variation in date palm plantlets regenerated from callus. 2nd International Conference on Environment and Industrial Innovation, Hong Kong.
3. Al-Ameri, A.A., Al-Qurainy, F., Gaafar, A.R.Z., Khan, S. and Nadeem, M. (2016). Molecular Identification of sex in Phoenix dactylifera using inter simple sequence repeat markers. BioMed Research International, 1: 1-5.
4. Al-Faifi, S.A., Migdadi, H.M., Algamdi, S.S., Khan, M.A., Ammar, M.H., Al-Obeed, R.S. and Jakse, J. (2016). Development, characterization and use of genomic SSR markers for assessment of genetic diversity in some Saudi date palm (Phoenix dactylifera L.) cultivars. Electronic Journal of Biotechnology, 21: 18-25.
5. Ali, T.A., Jasim, A.M. and Jubrail, J.M. (2006). The use of RAPDs for the detection of genetic stability of regenerated plantlets of Barhee palm in Iraq. 3rd International Date Palm Conference, Abu Dhabi, United Arab Emirates.
6. Al-Khalifah, N.S. (2006). Micro propagation and DNA fingerprinting of date palm trees of Saudi Arabia. Association of Agricultural Research Institutions in the Near East and North Africa, Amman, Jordan.
7. Al-Ruqaishi, I.A., Davey, M., Alderson, P. and Mayes, S. (2008). Genetic relationships and genotype tracing in date palms (Phoenix dactylifera L.) in Oman, based on microsatellite markers. Plant Genetic Resources, 6(1): 70-72.
8. Bader, S.M., Baum, M., Khierallah, H.S. and Choumane, W. (2007). The Use of RAPDs technique for the detection of genetic stability of date palm plantlets derived from In Vitro culture of Inflorescence. Education, 4: 151-159.
9. Elmeer, K. and Mattat, I. (2015). Genetic diversity of Qatari date palm using SSR markers. Genetics and Molecular Research, 14(1): 1624-1635.
10. Elmeer, K., Sarwath, H., Malek, J., Baum, M. and Hamwieh, A. (2011). New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.). Biotechnology, 1(2): 91-97.
11. Gheitarani, B., Erfani-Moghadam, J. and Fazeli, A. (2020). Evaluation of genetic diversity among some common fig using RAPD and ISSR molecular markers. Plant Genetic Researches, 6(2): 43-54 (In Persian).
12. Khierallah, H.S.M. (2015). Applications of Molecular Markers in Date Palm Genome Analysis and Breeding. Research Signpost, Kerala, IND.
13. Khierallah, H.S.M., Bader, S.M., Baum, M. and Choumane, W. (2008). Assessment of AFLP variations in date palm in vitro plantlets derived from inflorescence explant. The 4th Symposium Date Palm, Abu Dhabi, United Arab Emirates.
14. Khierallah, H.S., Bader, S.M., Hamwieh, A. and Baum, M. (2017). Date palm genetic diversity analysis using microsatellite polymorphism. Methods in Molecular Biology, 1638: 113-124 [DOI:10.1007/978-1-4939-7159-6_11]
15. Korir, N.K., Han, J., Shangguan, L., Wang, C., Kayesh, E., Zhang, Y. and Fang, J. (2013). Plant variety and cultivar identification: advances and prospects. Critical Reviews in Biotechnology, 33(2): 111-125.
16. Mardi, M., Torahi, A. and Kavand, A.R. (2010). Application of Microsatellite Markers for Identification and Registration of Date Palm Cultivars. (Final Report). Agricultural Research, Education and Extension Organization (AREEO), Karaj, IR (In Persian).
17. MirMohammadi Maibody, S.A.M. and Golkar, P. (2019). Application of DNA molecular markers in plant breeding. Plant Genetic Researches, 6(1):1-30 (In Persian).
18. Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A. and Allard R.W. (1984). Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proceedings of the National Academy of Sciences of the United States of America. 81: 8014-8019.
19. Saker, M.M., Bekheet, S.A., Taha, H.S., Fahmy, A.S. and Moursy, H.A. (2000). Detection of somaclonal variations in tissue culture-derived date palm plants using isozyme analysis and RAPD fingerprints. Biologia Plantarum, 43(3): 347-351.
20. Shatha, A.Y., Lina, A.H. and Tagreed, A.A.J. (2014). DNA fingerprinting of some Iraqi date palm (Phoenix dactylifera L.) cultivars. The 5th International Date Palm Conference, United Arab Emirates.
21. Zaid, A. and De Wet, P.F. (2002). Date palm propagation. In: Zaid, A., Eds., Date Palm Cultivation. pp: 73-105. Food and Agriculture Organization of the United Nations, Rome, IT.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rezaei M, Kavand A. Application of SSR Markers for Genetic Segregation of Some Commercial Date Palm Cultivars. pgr 2021; 7 (2) :97-108
URL: http://pgr.lu.ac.ir/article-1-169-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 7, Issue 2 (2021) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
Persian site map - English site map - Created in 0.06 seconds with 39 queries by YEKTAWEB 4642