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:: Volume 6, Issue 2 (2020) ::
pgr 2020, 6(2): 97-110 Back to browse issues page
Assessment of Diversity in Barley Genotypes (Hordeum vulgare L) Based on Beta-Glucan Content and ISSR Markers
Raheleh Aziznia , Hedieh Badakhshan * , Taimoor Javadi , Soma Zamani
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran , h.badakhshan@uok.ac.ir
Abstract:   (12599 Views)
In this study, variation of beta-glucan content was assessed in 20 barley line and cultivars based on complete block design with three replications. Genetic diversity of these genotypes was also evaluated using ISSR markers. Beta-glucan extracted by an enzymatic method. Significant differences were found at the level of 1% among barley genotypes for beta-glucan content. The beta glucan content was variable from 7.21 to 12.48 and, the Yosef, E94B3 and E94B17 genotypes hold the highest content of the beta-glucan. ISSR primers with average polymorphism of 66.79%, genetic diversity of 0.25 and Shannon index of 0.37 were determined as efficient markers for studying genetic diversity. The barley lines and cultivars were assigned in two distinct groups according to their genetic pedigree. On the basis of non-parametric Kruskal-Wallis, Spearman correlation, and stepwise regression analysis, nine informative primers were detected explaining highest seed’s beta-glucan content variations ranging from 24.3 to 42.4 percent. The ISSR6 (700), the combination of ISSR1+ISSR4 (1400) and IS2+ ISSR2 (1400) primers were the most informative primers for the beta-glucan content. The informative markers provide possible functional and efficient marker based selection method and, screening the barley germplasms for the highest beta-glucan content.
Keywords: Barley, Beta-glucan, Informative markers, Kruskal-Wallis, Stepwise regression
Full-Text [PDF 1127 kb]   (1239 Downloads)    
Type of Study: Research | Subject: Molecular genetics
Accepted: 2020/05/6
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Aziznia R, Badakhshan H, Javadi T, Zamani S. Assessment of Diversity in Barley Genotypes (Hordeum vulgare L) Based on Beta-Glucan Content and ISSR Markers. pgr 2020; 6 (2) :97-110
URL: http://pgr.lu.ac.ir/article-1-157-en.html


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Volume 6, Issue 2 (2020) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
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